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Redefining the intraepithelial lymphocytes threshold to diagnose gluten sensitivity in patients with architecturally normal duodenal histology.
Aliment Pharmacol Ther. 2011 Mar; 33(6):697-706.AP

Abstract

BACKGROUND

Accuracy of intraepithelial lymphocytes counts for diagnosing mild enteropathy coeliac disease in absence of villous atrophy can be limited by inappropriate controls included in the studies.

AIM

To determine the diagnostic accuracy of intraepithelial lymphocytes counts utilising controls lacking HLA coeliac disease-associated alleles.

METHODS

Intraepithelial lymphocytes counting at villus tip and per 100 enterocytes was performed at haematoxylin and eosin (H&E) and CD3-stainings in: 29 cases (21 with potential coeliac disease and 8 affected by latent coeliac disease) representing the patient population and 14 noncoeliac controls lacking HLA-DQ2/DQ8 alleles.

RESULTS

Threshold (mean+2 s.d.) of duodenal intraepithelial lymphocytes at villus tip and per 100 enterocytes in noncoeliac controls was respectively: 3.5 and 18 at H&E, 3.2 and 17 following CD3-staining. Considering the whole patient population, the sensitivity of tip intraepithelial lymphocytes in detecting mild enteropathy coeliac disease was 90% (95% CI=72.6-97.8) both at H&E and CD3-stainings. The sensitivity of intraepithelial lymphocytes per 100 enterocytes was 93% (95% CI=77.2-99.2) both at H&E and CD3-staining. Specificity of both intraepithelial lymphocytes counts was 100% (95% CI=76.8-100). Using a threshold of 25 intraepithelial lymphocytes per 100 enterocytes could miss 59% of cases at H&E and 48% following CD3-staining.

CONCLUSIONS

Intraepithelial lymphocytes counts are diagnostic feasible tools to detect mild enteropathy coeliac disease. Threshold of duodenal intraepithelial lymphocytes may be lower than currently accepted.

Authors+Show Affiliations

Regional Celiac Center, Pad. NI, University Hospital G. Martino, via Consolare Valeria 1, Messina, Italy. fumonero@inwind.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21255060

Citation

Pellegrino, S, et al. "Redefining the Intraepithelial Lymphocytes Threshold to Diagnose Gluten Sensitivity in Patients With Architecturally Normal Duodenal Histology." Alimentary Pharmacology & Therapeutics, vol. 33, no. 6, 2011, pp. 697-706.
Pellegrino S, Villanacci V, Sansotta N, et al. Redefining the intraepithelial lymphocytes threshold to diagnose gluten sensitivity in patients with architecturally normal duodenal histology. Aliment Pharmacol Ther. 2011;33(6):697-706.
Pellegrino, S., Villanacci, V., Sansotta, N., Scarfì, R., Bassotti, G., Vieni, G., Princiotta, A., Sferlazzas, C., Magazzù, G., & Tuccari, G. (2011). Redefining the intraepithelial lymphocytes threshold to diagnose gluten sensitivity in patients with architecturally normal duodenal histology. Alimentary Pharmacology & Therapeutics, 33(6), 697-706. https://doi.org/10.1111/j.1365-2036.2011.04578.x
Pellegrino S, et al. Redefining the Intraepithelial Lymphocytes Threshold to Diagnose Gluten Sensitivity in Patients With Architecturally Normal Duodenal Histology. Aliment Pharmacol Ther. 2011;33(6):697-706. PubMed PMID: 21255060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Redefining the intraepithelial lymphocytes threshold to diagnose gluten sensitivity in patients with architecturally normal duodenal histology. AU - Pellegrino,S, AU - Villanacci,V, AU - Sansotta,N, AU - Scarfì,R, AU - Bassotti,G, AU - Vieni,G, AU - Princiotta,A, AU - Sferlazzas,C, AU - Magazzù,G, AU - Tuccari,G, Y1 - 2011/01/23/ PY - 2011/1/25/entrez PY - 2011/1/25/pubmed PY - 2011/6/4/medline SP - 697 EP - 706 JF - Alimentary pharmacology & therapeutics JO - Aliment Pharmacol Ther VL - 33 IS - 6 N2 - BACKGROUND: Accuracy of intraepithelial lymphocytes counts for diagnosing mild enteropathy coeliac disease in absence of villous atrophy can be limited by inappropriate controls included in the studies. AIM: To determine the diagnostic accuracy of intraepithelial lymphocytes counts utilising controls lacking HLA coeliac disease-associated alleles. METHODS: Intraepithelial lymphocytes counting at villus tip and per 100 enterocytes was performed at haematoxylin and eosin (H&E) and CD3-stainings in: 29 cases (21 with potential coeliac disease and 8 affected by latent coeliac disease) representing the patient population and 14 noncoeliac controls lacking HLA-DQ2/DQ8 alleles. RESULTS: Threshold (mean+2 s.d.) of duodenal intraepithelial lymphocytes at villus tip and per 100 enterocytes in noncoeliac controls was respectively: 3.5 and 18 at H&E, 3.2 and 17 following CD3-staining. Considering the whole patient population, the sensitivity of tip intraepithelial lymphocytes in detecting mild enteropathy coeliac disease was 90% (95% CI=72.6-97.8) both at H&E and CD3-stainings. The sensitivity of intraepithelial lymphocytes per 100 enterocytes was 93% (95% CI=77.2-99.2) both at H&E and CD3-staining. Specificity of both intraepithelial lymphocytes counts was 100% (95% CI=76.8-100). Using a threshold of 25 intraepithelial lymphocytes per 100 enterocytes could miss 59% of cases at H&E and 48% following CD3-staining. CONCLUSIONS: Intraepithelial lymphocytes counts are diagnostic feasible tools to detect mild enteropathy coeliac disease. Threshold of duodenal intraepithelial lymphocytes may be lower than currently accepted. SN - 1365-2036 UR - https://www.unboundmedicine.com/medline/citation/21255060/Redefining_the_intraepithelial_lymphocytes_threshold_to_diagnose_gluten_sensitivity_in_patients_with_architecturally_normal_duodenal_histology_ L2 - https://doi.org/10.1111/j.1365-2036.2011.04578.x DB - PRIME DP - Unbound Medicine ER -