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QCM immunosensor detection of Escherichia coli O157:H7 based on beacon immunomagnetic nanoparticles and catalytic growth of colloidal gold.
Biosens Bioelectron. 2011 Mar 15; 26(7):3376-81.BB

Abstract

In this paper, we describe a novel method for detecting Escherichia coli (E. coli) O157:H7 by using a quartz crystal microbalance (QCM) immunosensor based on beacon immunomagnetic nanoparticles (BIMPs), streptavidin-gold, and growth solution. E. coli O157-BIMPs were magnetic nanoparticles loaded with polyclonal anti-E. coli O157:H7 antibody (target antibody, T-Ab) and biotin-IgG (beacon antibody, B-Ab) at an optimized ratio of 1:60 (T-Ab:B-Ab). E. coli O157:H7 was captured and separated by E. coli O157-BIMPs in a sample, and the streptavidin-gold was subsequently conjugated to E. coli O157-BIMPs by using a biotin-avidin system. Finally, the gold particles on E. coli O157-BIMPs were enlarged in growth solution, and the compounds containing E. coli O157:H7, E. coli O157-BIMPs, and enlarged gold particles were collected using a magnetic plate. The QCM immunosensor was fabricated with protein A from Staphylococcus aureus and monoclonal anti-E. coli O157:H7 antibody. The compounds decreased the immunosensor's resonant frequency. E. coli O157-BIMPs and enlarged gold particles were used as "mass enhancers" to amplify the frequency change. The frequency shift was correlated to the bacterial concentration. The detection limit was 23 CFU/ml in phosphate-buffered saline and 53 CFU/ml in milk. This method could successfully detect E. coli O157:H7 with high specificity and stability. The entire procedure for the detection of E. coli O157:H7 took only 4 h.

Authors+Show Affiliations

Department of Environment and Health, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21256727

Citation

Shen, Zhi-Qiang, et al. "QCM Immunosensor Detection of Escherichia Coli O157:H7 Based On Beacon Immunomagnetic Nanoparticles and Catalytic Growth of Colloidal Gold." Biosensors & Bioelectronics, vol. 26, no. 7, 2011, pp. 3376-81.
Shen ZQ, Wang JF, Qiu ZG, et al. QCM immunosensor detection of Escherichia coli O157:H7 based on beacon immunomagnetic nanoparticles and catalytic growth of colloidal gold. Biosens Bioelectron. 2011;26(7):3376-81.
Shen, Z. Q., Wang, J. F., Qiu, Z. G., Jin, M., Wang, X. W., Chen, Z. L., Li, J. W., & Cao, F. H. (2011). QCM immunosensor detection of Escherichia coli O157:H7 based on beacon immunomagnetic nanoparticles and catalytic growth of colloidal gold. Biosensors & Bioelectronics, 26(7), 3376-81. https://doi.org/10.1016/j.bios.2010.12.035
Shen ZQ, et al. QCM Immunosensor Detection of Escherichia Coli O157:H7 Based On Beacon Immunomagnetic Nanoparticles and Catalytic Growth of Colloidal Gold. Biosens Bioelectron. 2011 Mar 15;26(7):3376-81. PubMed PMID: 21256727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - QCM immunosensor detection of Escherichia coli O157:H7 based on beacon immunomagnetic nanoparticles and catalytic growth of colloidal gold. AU - Shen,Zhi-Qiang, AU - Wang,Jing-Feng, AU - Qiu,Zhi-Gang, AU - Jin,Min, AU - Wang,Xin-Wei, AU - Chen,Zhao-Li, AU - Li,Jun-Wen, AU - Cao,Fu-Huan, Y1 - 2010/12/30/ PY - 2010/10/08/received PY - 2010/12/22/revised PY - 2010/12/23/accepted PY - 2011/1/25/entrez PY - 2011/1/25/pubmed PY - 2011/6/7/medline SP - 3376 EP - 81 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 7 N2 - In this paper, we describe a novel method for detecting Escherichia coli (E. coli) O157:H7 by using a quartz crystal microbalance (QCM) immunosensor based on beacon immunomagnetic nanoparticles (BIMPs), streptavidin-gold, and growth solution. E. coli O157-BIMPs were magnetic nanoparticles loaded with polyclonal anti-E. coli O157:H7 antibody (target antibody, T-Ab) and biotin-IgG (beacon antibody, B-Ab) at an optimized ratio of 1:60 (T-Ab:B-Ab). E. coli O157:H7 was captured and separated by E. coli O157-BIMPs in a sample, and the streptavidin-gold was subsequently conjugated to E. coli O157-BIMPs by using a biotin-avidin system. Finally, the gold particles on E. coli O157-BIMPs were enlarged in growth solution, and the compounds containing E. coli O157:H7, E. coli O157-BIMPs, and enlarged gold particles were collected using a magnetic plate. The QCM immunosensor was fabricated with protein A from Staphylococcus aureus and monoclonal anti-E. coli O157:H7 antibody. The compounds decreased the immunosensor's resonant frequency. E. coli O157-BIMPs and enlarged gold particles were used as "mass enhancers" to amplify the frequency change. The frequency shift was correlated to the bacterial concentration. The detection limit was 23 CFU/ml in phosphate-buffered saline and 53 CFU/ml in milk. This method could successfully detect E. coli O157:H7 with high specificity and stability. The entire procedure for the detection of E. coli O157:H7 took only 4 h. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21256727/QCM_immunosensor_detection_of_Escherichia_coli_O157:H7_based_on_beacon_immunomagnetic_nanoparticles_and_catalytic_growth_of_colloidal_gold_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00870-5 DB - PRIME DP - Unbound Medicine ER -