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Analysis of antioxidant metabolites by solvent extraction from sclerotia of Inonotus obliquus (Chaga).
Phytochem Anal. 2011 Mar-Apr; 22(2):95-102.PA

Abstract

INTRODUCTION

The sclerotia of Inonotus obliquus (Chaga) are effective therapeutic agents to treat several human malignant tumours and other diseases without unacceptable toxic side-effects.

OBJECTIVE

To investigate solvent effects on metabolic profiles and antioxidant activities of extracts of Chaga.

METHODOLOGY

Chaga was extracted by petroleum ether, chloroform, ethyl acetate, acetone, ethanol and water. Solvent effects on metabolites in the extracts were assayed by NMR-based metabolomic analysis. Antioxidant activities were indicated as capacities for scavenging superoxide anion, DPPH and hydroxyl radicals.

RESULTS

Petroleum ether and chloroform extracts contained primarily lanostane-type triterpenoids (LT), whereas the extracts of ethyl acetate, acetone and ethanol were characterised by the predominant presence of hispidin analogues and LT, and water extracts by polysaccharides and phenolic compounds. The ethyl acetate, acetone, ethanol and water extracts revealed remarkable potential for scavenging the tested radicals, while those of petroleum ether and chloroform did not. Polyphenols are the major contributors for quenching the tested free radicals, while in LT only compounds 16, 17 and 22 participated in scavenging hydroxyl radicals.

CONCLUSION

Polyphenols in Chaga are the principles for quenching free radicals while polysaccharides and a few LT compounds contribute partially in scavenging DPPH and hydroxyl radicals, respectively. NMR-based metabolomic analysis is a useful method by which to correlate ¹H-NMR spectra of Chaga extracts with their antioxidant activities, and this allows the prediction of potentials for scavenging free radicals by ¹H-NMR spectroscopy.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zheng W
Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou 221116, China. yyzw@xznu.edu.cn
Zhang M
No affiliation info available
Zhao Y
No affiliation info available
Miao K
No affiliation info available
Pan S
No affiliation info available
Cao F
No affiliation info available
Dai Y
No affiliation info available

MeSH

AntioxidantsBasidiomycotaFree Radical ScavengersFruiting Bodies, FungalHydroxyl RadicalMagnetic Resonance SpectroscopyMetabolomicsPolyphenolsSolventsSuperoxides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21259372

Citation

Zheng, Weifa, et al. "Analysis of Antioxidant Metabolites By Solvent Extraction From Sclerotia of Inonotus Obliquus (Chaga)." Phytochemical Analysis : PCA, vol. 22, no. 2, 2011, pp. 95-102.
Zheng W, Zhang M, Zhao Y, et al. Analysis of antioxidant metabolites by solvent extraction from sclerotia of Inonotus obliquus (Chaga). Phytochem Anal. 2011;22(2):95-102.
Zheng, W., Zhang, M., Zhao, Y., Miao, K., Pan, S., Cao, F., & Dai, Y. (2011). Analysis of antioxidant metabolites by solvent extraction from sclerotia of Inonotus obliquus (Chaga). Phytochemical Analysis : PCA, 22(2), 95-102. https://doi.org/10.1002/pca.1225
Zheng W, et al. Analysis of Antioxidant Metabolites By Solvent Extraction From Sclerotia of Inonotus Obliquus (Chaga). Phytochem Anal. 2011 Mar-Apr;22(2):95-102. PubMed PMID: 21259372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of antioxidant metabolites by solvent extraction from sclerotia of Inonotus obliquus (Chaga). AU - Zheng,Weifa, AU - Zhang,Meimei, AU - Zhao,Yanxia, AU - Miao,Kangjie, AU - Pan,Shenyuan, AU - Cao,Feng, AU - Dai,Yucheng, Y1 - 2011/01/24/ PY - 2009/10/12/received PY - 2010/02/24/revised PY - 2010/02/25/accepted PY - 2011/1/25/entrez PY - 2011/1/25/pubmed PY - 2012/3/27/medline SP - 95 EP - 102 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 22 IS - 2 N2 - INTRODUCTION: The sclerotia of Inonotus obliquus (Chaga) are effective therapeutic agents to treat several human malignant tumours and other diseases without unacceptable toxic side-effects. OBJECTIVE: To investigate solvent effects on metabolic profiles and antioxidant activities of extracts of Chaga. METHODOLOGY: Chaga was extracted by petroleum ether, chloroform, ethyl acetate, acetone, ethanol and water. Solvent effects on metabolites in the extracts were assayed by NMR-based metabolomic analysis. Antioxidant activities were indicated as capacities for scavenging superoxide anion, DPPH and hydroxyl radicals. RESULTS: Petroleum ether and chloroform extracts contained primarily lanostane-type triterpenoids (LT), whereas the extracts of ethyl acetate, acetone and ethanol were characterised by the predominant presence of hispidin analogues and LT, and water extracts by polysaccharides and phenolic compounds. The ethyl acetate, acetone, ethanol and water extracts revealed remarkable potential for scavenging the tested radicals, while those of petroleum ether and chloroform did not. Polyphenols are the major contributors for quenching the tested free radicals, while in LT only compounds 16, 17 and 22 participated in scavenging hydroxyl radicals. CONCLUSION: Polyphenols in Chaga are the principles for quenching free radicals while polysaccharides and a few LT compounds contribute partially in scavenging DPPH and hydroxyl radicals, respectively. NMR-based metabolomic analysis is a useful method by which to correlate ¹H-NMR spectra of Chaga extracts with their antioxidant activities, and this allows the prediction of potentials for scavenging free radicals by ¹H-NMR spectroscopy. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/21259372/Analysis_of_antioxidant_metabolites_by_solvent_extraction_from_sclerotia_of_Inonotus_obliquus__Chaga__ L2 - https://doi.org/10.1002/pca.1225 DB - PRIME DP - Unbound Medicine ER -