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Effects on sleep and dopamine levels of microdialysis perfusion of cannabidiol into the lateral hypothalamus of rats.

Abstract

AIMS

The major non-psychoactive component of Cannabis sativa, cannabidiol (CBD), displays a plethora of actions including wakefulness. In the present study, we addressed whether perfusing CBD via microdialysis into lateral hypothalamus (LH) during the lights-on period would modify the sleep-wake cycle of rats as well as the contents of dopamine (DA) collected from nucleus accumbens (AcbC). Additionally, we tested whether perfusion of CBD into LH would block the sleep rebound after a sleep deprivation period.

MAIN METHODS

Electroencephalogram and electromyogram electrodes were implanted in rats as well as a guide-cannula aimed to LH or AcbC. CBD perfusion was carried out via cannulae placed into LH whereas contents of DA were collected from AcbC and analyzed using HPLC means.

KEY FINDINGS

We found that microdialysis perfusion of CBD (30, 60, or 90 nM) into LH of rat enhances alertness and suppresses sleep. This effect was accompanied with an increase in DA extracellular levels collected from the AcbC. Furthermore, perfusion of CBD into LH after total sleep deprivation prevented the sleep rebound.

SIGNIFICANCE

These findings enhance the investigation about the neurobiological properties of CBD on sleep modulation.

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  • Authors+Show Affiliations

    ,

    Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de Salud, Universidad Anáhuac Mayab, Mérida, Yucatán, México. eric.murillo@anahuac.mx

    , , ,

    Source

    Life sciences 88:11-12 2011 Mar 14 pg 504-11

    MeSH

    Animals
    Cannabidiol
    Dopamine
    Electrodes, Implanted
    Electroencephalography
    Electromyography
    Hypothalamus
    Male
    Microdialysis
    Nucleus Accumbens
    Perfusion
    Rats
    Rats, Wistar
    Sleep
    Sleep Deprivation

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    21262236

    Citation

    Murillo-Rodríguez, Eric, et al. "Effects On Sleep and Dopamine Levels of Microdialysis Perfusion of Cannabidiol Into the Lateral Hypothalamus of Rats." Life Sciences, vol. 88, no. 11-12, 2011, pp. 504-11.
    Murillo-Rodríguez E, Palomero-Rivero M, Millán-Aldaco D, et al. Effects on sleep and dopamine levels of microdialysis perfusion of cannabidiol into the lateral hypothalamus of rats. Life Sci. 2011;88(11-12):504-11.
    Murillo-Rodríguez, E., Palomero-Rivero, M., Millán-Aldaco, D., Mechoulam, R., & Drucker-Colín, R. (2011). Effects on sleep and dopamine levels of microdialysis perfusion of cannabidiol into the lateral hypothalamus of rats. Life Sciences, 88(11-12), pp. 504-11. doi:10.1016/j.lfs.2011.01.013.
    Murillo-Rodríguez E, et al. Effects On Sleep and Dopamine Levels of Microdialysis Perfusion of Cannabidiol Into the Lateral Hypothalamus of Rats. Life Sci. 2011 Mar 14;88(11-12):504-11. PubMed PMID: 21262236.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects on sleep and dopamine levels of microdialysis perfusion of cannabidiol into the lateral hypothalamus of rats. AU - Murillo-Rodríguez,Eric, AU - Palomero-Rivero,Marcela, AU - Millán-Aldaco,Diana, AU - Mechoulam,Raphael, AU - Drucker-Colín,René, Y1 - 2011/01/22/ PY - 2010/10/08/received PY - 2010/12/09/revised PY - 2010/12/27/accepted PY - 2011/1/26/entrez PY - 2011/1/26/pubmed PY - 2011/5/7/medline SP - 504 EP - 11 JF - Life sciences JO - Life Sci. VL - 88 IS - 11-12 N2 - AIMS: The major non-psychoactive component of Cannabis sativa, cannabidiol (CBD), displays a plethora of actions including wakefulness. In the present study, we addressed whether perfusing CBD via microdialysis into lateral hypothalamus (LH) during the lights-on period would modify the sleep-wake cycle of rats as well as the contents of dopamine (DA) collected from nucleus accumbens (AcbC). Additionally, we tested whether perfusion of CBD into LH would block the sleep rebound after a sleep deprivation period. MAIN METHODS: Electroencephalogram and electromyogram electrodes were implanted in rats as well as a guide-cannula aimed to LH or AcbC. CBD perfusion was carried out via cannulae placed into LH whereas contents of DA were collected from AcbC and analyzed using HPLC means. KEY FINDINGS: We found that microdialysis perfusion of CBD (30, 60, or 90 nM) into LH of rat enhances alertness and suppresses sleep. This effect was accompanied with an increase in DA extracellular levels collected from the AcbC. Furthermore, perfusion of CBD into LH after total sleep deprivation prevented the sleep rebound. SIGNIFICANCE: These findings enhance the investigation about the neurobiological properties of CBD on sleep modulation. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/21262236/Effects_on_sleep_and_dopamine_levels_of_microdialysis_perfusion_of_cannabidiol_into_the_lateral_hypothalamus_of_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(11)00033-6 DB - PRIME DP - Unbound Medicine ER -