Tags

Type your tag names separated by a space and hit enter

Analysis of volatile organic compounds (VOCs) in the headspace of NCI-H1666 lung cancer cells.

Abstract

Analysis of volatile organic compounds (VOCs) provides an elegant approach for cancer screening and disease monitoring, whose use is currently limited by a lack of validated cancer-derived metabolites, which may serve as biomarkers. The aim of the experiments presented here was to investigate the release and consumption of VOCs from the non small cell lung cancer cell line NCI-H1666, which was originally derived from a bronchoalveolar carcinoma.Following detachment by trypsinization suspended cells were incubated in a sealed fermenter for 21 hours. 200 ml of headspace from the cell culture were sampled, diluted with dry, highly purified air and preconcentrated by adsorption on three different solid sorbents with increasing adsorption strength. VOC-analysis was performed by thermodesorption-gas chromatography mass spectrometry (TD-GC-MS). In contrast to our previous studies experiments with NCI-H1666 cells only confirmed the consumption of several aldehydes, n-butyl acetate and the ethers methyl tert-butyl ether and ethyl tert-butyl ether, but no unequivocal release of VOCs was observed. Together with our previously published work these data indicate that the consumption of certain VOCs is commonly observed while their release shows cell line-restricted patterns, whose underlying causes are unknown.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Univ.-Clinic for Anesthesia, Anichstrasse 35, 6020 Innsbruck, Austria.

    , , , , ,

    Source

    MeSH

    Acetates
    Adenocarcinoma
    Adenocarcinoma of Lung
    Carcinoma, Non-Small-Cell Lung
    Cell Line, Tumor
    Ethyl Ethers
    Gas Chromatography-Mass Spectrometry
    Humans
    Intracellular Space
    Lung Neoplasms
    Methyl Ethers
    Osmolar Concentration
    Volatile Organic Compounds

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Validation Studies

    Language

    eng

    PubMed ID

    21263191

    Citation

    Sponring, Andreas, et al. "Analysis of Volatile Organic Compounds (VOCs) in the Headspace of NCI-H1666 Lung Cancer Cells." Cancer Biomarkers : Section a of Disease Markers, vol. 7, no. 3, 2010, pp. 153-61.
    Sponring A, Filipiak W, Ager C, et al. Analysis of volatile organic compounds (VOCs) in the headspace of NCI-H1666 lung cancer cells. Cancer Biomark. 2010;7(3):153-61.
    Sponring, A., Filipiak, W., Ager, C., Schubert, J., Miekisch, W., Amann, A., & Troppmair, J. (2010). Analysis of volatile organic compounds (VOCs) in the headspace of NCI-H1666 lung cancer cells. Cancer Biomarkers : Section a of Disease Markers, 7(3), pp. 153-61. doi:10.3233/CBM-2010-0182.
    Sponring A, et al. Analysis of Volatile Organic Compounds (VOCs) in the Headspace of NCI-H1666 Lung Cancer Cells. Cancer Biomark. 2010;7(3):153-61. PubMed PMID: 21263191.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Analysis of volatile organic compounds (VOCs) in the headspace of NCI-H1666 lung cancer cells. AU - Sponring,Andreas, AU - Filipiak,Wojciech, AU - Ager,Clemens, AU - Schubert,Jochen, AU - Miekisch,Wolfram, AU - Amann,Anton, AU - Troppmair,Jakob, PY - 2011/1/26/entrez PY - 2011/1/26/pubmed PY - 2011/5/5/medline SP - 153 EP - 61 JF - Cancer biomarkers : section A of Disease markers JO - Cancer Biomark VL - 7 IS - 3 N2 - Analysis of volatile organic compounds (VOCs) provides an elegant approach for cancer screening and disease monitoring, whose use is currently limited by a lack of validated cancer-derived metabolites, which may serve as biomarkers. The aim of the experiments presented here was to investigate the release and consumption of VOCs from the non small cell lung cancer cell line NCI-H1666, which was originally derived from a bronchoalveolar carcinoma.Following detachment by trypsinization suspended cells were incubated in a sealed fermenter for 21 hours. 200 ml of headspace from the cell culture were sampled, diluted with dry, highly purified air and preconcentrated by adsorption on three different solid sorbents with increasing adsorption strength. VOC-analysis was performed by thermodesorption-gas chromatography mass spectrometry (TD-GC-MS). In contrast to our previous studies experiments with NCI-H1666 cells only confirmed the consumption of several aldehydes, n-butyl acetate and the ethers methyl tert-butyl ether and ethyl tert-butyl ether, but no unequivocal release of VOCs was observed. Together with our previously published work these data indicate that the consumption of certain VOCs is commonly observed while their release shows cell line-restricted patterns, whose underlying causes are unknown. SN - 1875-8592 UR - https://www.unboundmedicine.com/medline/citation/21263191/abstract/Analysis_of_volatile_organic_compounds__VOCs__in_the_headspace_of_NCI_H1666_lung_cancer_cells_ L2 - https://content.iospress.com/openurl?genre=article&id=doi:10.3233/CBM-2010-0182 DB - PRIME DP - Unbound Medicine ER -