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Selenium-induced up-regulation of the antioxidant defense and methylglyoxal detoxification system reduces salinity-induced damage in rapeseed seedlings.
Biol Trace Elem Res. 2011 Dec; 143(3):1704-21.BT

Abstract

The present study investigates the regulatory role of exogenous selenium (Se) in the antioxidant defense and methylglyoxal (MG) detoxification systems in rapeseed seedlings exposed to salt stress. Twelve-day-old seedlings, grown in Petri dishes, were supplemented with selenium (25 μM Na(2)SeO(4)) and salt (100 and 200 mM NaCl) separately and in combination, and further grown for 48 h. The ascorbate (AsA) content of the seedlings decreased significantly with increased salt stress. The amount of reduced glutathione (GSH) and glutathione disulfide (GSSG) increased with an increase in the level of salt stress, while the GSH/GSSG ratio decreased. In addition, the ascorbate peroxidase (APX) and glutathione S-transferase (GST) activity increased significantly with increased salt concentration (both at 100 and 200 mM NaCl), while glutathione peroxidase (GPX) activity increased only at moderate salt stress (100 mM NaCl). Glutathione reductase (GR) activity remained unchanged at 100 mM NaCl, while it was decreased under severe (200 mM NaCl) salt stress. Monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), catalase (CAT), glyoxalase I (Gly I), and glyoxalase II (Gly II) activities decreased upon the imposition of salt stress, whereas a sharp decrease of these activities was observed under severe salt stress (200 mM NaCl). Concomitant increases in the levels of H(2)O(2) and lipid peroxidation (MDA) were also measured. Exogenous Se treatment alone had little effect on the non-enzymatic and enzymatic components. However, further investigation revealed that Se treatment had a synergistic effect: in salt-stressed seedlings, it increased the AsA and GSH contents; GSH/GSSG ratio; and the activities of APX, MDHAR, DHAR, GR, GST, GPX, CAT, Gly I, and Gly II. As a result, addition of Se in salt-stressed seedlings led to a reduction in the levels of H(2)O(2) and MDA as compared to salt stress alone. These results suggest that the exogenous application of Se rendered the plants more tolerant to salt stress-induced oxidative damage by enhancing their antioxidant defense and MG detoxification systems.

Authors+Show Affiliations

Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan. mhzsauag@gmail.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21264525

Citation

Hasanuzzaman, Mirza, et al. "Selenium-induced Up-regulation of the Antioxidant Defense and Methylglyoxal Detoxification System Reduces Salinity-induced Damage in Rapeseed Seedlings." Biological Trace Element Research, vol. 143, no. 3, 2011, pp. 1704-21.
Hasanuzzaman M, Hossain MA, Fujita M. Selenium-induced up-regulation of the antioxidant defense and methylglyoxal detoxification system reduces salinity-induced damage in rapeseed seedlings. Biol Trace Elem Res. 2011;143(3):1704-21.
Hasanuzzaman, M., Hossain, M. A., & Fujita, M. (2011). Selenium-induced up-regulation of the antioxidant defense and methylglyoxal detoxification system reduces salinity-induced damage in rapeseed seedlings. Biological Trace Element Research, 143(3), 1704-21. https://doi.org/10.1007/s12011-011-8958-4
Hasanuzzaman M, Hossain MA, Fujita M. Selenium-induced Up-regulation of the Antioxidant Defense and Methylglyoxal Detoxification System Reduces Salinity-induced Damage in Rapeseed Seedlings. Biol Trace Elem Res. 2011;143(3):1704-21. PubMed PMID: 21264525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium-induced up-regulation of the antioxidant defense and methylglyoxal detoxification system reduces salinity-induced damage in rapeseed seedlings. AU - Hasanuzzaman,Mirza, AU - Hossain,Mohammad Anwar, AU - Fujita,Masayuki, Y1 - 2011/01/25/ PY - 2010/12/12/received PY - 2011/01/05/accepted PY - 2011/1/26/entrez PY - 2011/1/26/pubmed PY - 2012/3/17/medline SP - 1704 EP - 21 JF - Biological trace element research JO - Biol Trace Elem Res VL - 143 IS - 3 N2 - The present study investigates the regulatory role of exogenous selenium (Se) in the antioxidant defense and methylglyoxal (MG) detoxification systems in rapeseed seedlings exposed to salt stress. Twelve-day-old seedlings, grown in Petri dishes, were supplemented with selenium (25 μM Na(2)SeO(4)) and salt (100 and 200 mM NaCl) separately and in combination, and further grown for 48 h. The ascorbate (AsA) content of the seedlings decreased significantly with increased salt stress. The amount of reduced glutathione (GSH) and glutathione disulfide (GSSG) increased with an increase in the level of salt stress, while the GSH/GSSG ratio decreased. In addition, the ascorbate peroxidase (APX) and glutathione S-transferase (GST) activity increased significantly with increased salt concentration (both at 100 and 200 mM NaCl), while glutathione peroxidase (GPX) activity increased only at moderate salt stress (100 mM NaCl). Glutathione reductase (GR) activity remained unchanged at 100 mM NaCl, while it was decreased under severe (200 mM NaCl) salt stress. Monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), catalase (CAT), glyoxalase I (Gly I), and glyoxalase II (Gly II) activities decreased upon the imposition of salt stress, whereas a sharp decrease of these activities was observed under severe salt stress (200 mM NaCl). Concomitant increases in the levels of H(2)O(2) and lipid peroxidation (MDA) were also measured. Exogenous Se treatment alone had little effect on the non-enzymatic and enzymatic components. However, further investigation revealed that Se treatment had a synergistic effect: in salt-stressed seedlings, it increased the AsA and GSH contents; GSH/GSSG ratio; and the activities of APX, MDHAR, DHAR, GR, GST, GPX, CAT, Gly I, and Gly II. As a result, addition of Se in salt-stressed seedlings led to a reduction in the levels of H(2)O(2) and MDA as compared to salt stress alone. These results suggest that the exogenous application of Se rendered the plants more tolerant to salt stress-induced oxidative damage by enhancing their antioxidant defense and MG detoxification systems. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/21264525/Selenium_induced_up_regulation_of_the_antioxidant_defense_and_methylglyoxal_detoxification_system_reduces_salinity_induced_damage_in_rapeseed_seedlings_ L2 - https://dx.doi.org/10.1007/s12011-011-8958-4 DB - PRIME DP - Unbound Medicine ER -