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Prevalence of norovirus GII-4 antibodies in Finnish children.
J Med Virol. 2011 Mar; 83(3):525-31.JM

Abstract

Noroviruses (NoVs) are the second most common cause of viral gastroenteritis after rotavirus in children. NoV genotype GII-4 has emerged as the major type not only in outbreaks of NoV gastroenteritis but also endemic gastroenteritis among infants and young children worldwide. Using baculovirus-insect cell system virus-like particles (VLPs) of NoV genotype GII-4 and an uncommon genotype GII-12 were produced. These VLPs were used in enzyme-linked immunosorbent assays (ELISA) for detection of NoV-specific immunoglobulin G (IgG) and IgA antibodies in 492 serum specimens from Finnish children 0-14 years of age collected between 2006 and 2008. NoV IgG antibody prevalence was 47.3% in the age group 7-23 months and increased up to 91.2% after the age of 5 years. Avidity of NoV IgG antibodies was low in the primary infections while high avidity antibodies were detected in the recurrent infections of the older children. In GII-4 infections, the homologous antibody response to GII-4 VLPs was stronger than to GII-12 VLPs but cross-reactivity between GII-4 and GII-12 was observed. Binding of GII-4 VLPs to a putative carbohydrate antigen receptor H-type 3 could be blocked by sera from children not infected with NoV during a waterborne outbreak of acute gastroenteritis. Therefore, protection against NoV infection correlated with strong blocking activity.

Authors+Show Affiliations

Vaccine Research Center, University of Tampere Medical School, Tampere, Finland. kirsi.nurminen@uta.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21264875

Citation

Nurminen, K, et al. "Prevalence of Norovirus GII-4 Antibodies in Finnish Children." Journal of Medical Virology, vol. 83, no. 3, 2011, pp. 525-31.
Nurminen K, Blazevic V, Huhti L, et al. Prevalence of norovirus GII-4 antibodies in Finnish children. J Med Virol. 2011;83(3):525-31.
Nurminen, K., Blazevic, V., Huhti, L., Räsänen, S., Koho, T., Hytönen, V. P., & Vesikari, T. (2011). Prevalence of norovirus GII-4 antibodies in Finnish children. Journal of Medical Virology, 83(3), 525-31. https://doi.org/10.1002/jmv.21990
Nurminen K, et al. Prevalence of Norovirus GII-4 Antibodies in Finnish Children. J Med Virol. 2011;83(3):525-31. PubMed PMID: 21264875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of norovirus GII-4 antibodies in Finnish children. AU - Nurminen,K, AU - Blazevic,V, AU - Huhti,L, AU - Räsänen,S, AU - Koho,T, AU - Hytönen,V P, AU - Vesikari,T, PY - 2011/1/26/entrez PY - 2011/1/26/pubmed PY - 2011/12/17/medline SP - 525 EP - 31 JF - Journal of medical virology JO - J Med Virol VL - 83 IS - 3 N2 - Noroviruses (NoVs) are the second most common cause of viral gastroenteritis after rotavirus in children. NoV genotype GII-4 has emerged as the major type not only in outbreaks of NoV gastroenteritis but also endemic gastroenteritis among infants and young children worldwide. Using baculovirus-insect cell system virus-like particles (VLPs) of NoV genotype GII-4 and an uncommon genotype GII-12 were produced. These VLPs were used in enzyme-linked immunosorbent assays (ELISA) for detection of NoV-specific immunoglobulin G (IgG) and IgA antibodies in 492 serum specimens from Finnish children 0-14 years of age collected between 2006 and 2008. NoV IgG antibody prevalence was 47.3% in the age group 7-23 months and increased up to 91.2% after the age of 5 years. Avidity of NoV IgG antibodies was low in the primary infections while high avidity antibodies were detected in the recurrent infections of the older children. In GII-4 infections, the homologous antibody response to GII-4 VLPs was stronger than to GII-12 VLPs but cross-reactivity between GII-4 and GII-12 was observed. Binding of GII-4 VLPs to a putative carbohydrate antigen receptor H-type 3 could be blocked by sera from children not infected with NoV during a waterborne outbreak of acute gastroenteritis. Therefore, protection against NoV infection correlated with strong blocking activity. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/21264875/Prevalence_of_norovirus_GII_4_antibodies_in_Finnish_children_ L2 - https://doi.org/10.1002/jmv.21990 DB - PRIME DP - Unbound Medicine ER -