Tags

Type your tag names separated by a space and hit enter

Prevalence of norovirus GII-4 antibodies in Finnish children.
J Med Virol. 2011 Mar; 83(3):525-31.JM

Abstract

Noroviruses (NoVs) are the second most common cause of viral gastroenteritis after rotavirus in children. NoV genotype GII-4 has emerged as the major type not only in outbreaks of NoV gastroenteritis but also endemic gastroenteritis among infants and young children worldwide. Using baculovirus-insect cell system virus-like particles (VLPs) of NoV genotype GII-4 and an uncommon genotype GII-12 were produced. These VLPs were used in enzyme-linked immunosorbent assays (ELISA) for detection of NoV-specific immunoglobulin G (IgG) and IgA antibodies in 492 serum specimens from Finnish children 0-14 years of age collected between 2006 and 2008. NoV IgG antibody prevalence was 47.3% in the age group 7-23 months and increased up to 91.2% after the age of 5 years. Avidity of NoV IgG antibodies was low in the primary infections while high avidity antibodies were detected in the recurrent infections of the older children. In GII-4 infections, the homologous antibody response to GII-4 VLPs was stronger than to GII-12 VLPs but cross-reactivity between GII-4 and GII-12 was observed. Binding of GII-4 VLPs to a putative carbohydrate antigen receptor H-type 3 could be blocked by sera from children not infected with NoV during a waterborne outbreak of acute gastroenteritis. Therefore, protection against NoV infection correlated with strong blocking activity.

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Nurminen K
Vaccine Research Center, University of Tampere Medical School, Tampere, Finland. kirsi.nurminen@uta.fi
Blazevic V
No affiliation info available
Huhti L
No affiliation info available
Räsänen S
No affiliation info available
Koho T
No affiliation info available
Hytönen VP
No affiliation info available
Vesikari T
No affiliation info available

MeSH

Acute DiseaseAdolescentAge FactorsAntibodies, ViralAntibody AffinityCaliciviridae InfectionsChildChild, PreschoolCross ReactionsFinlandGastroenteritisGenotypeHumansInfantInfant, NewbornNorovirusPrevalenceSeroepidemiologic Studies

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21264875

Citation

Nurminen, K, et al. "Prevalence of Norovirus GII-4 Antibodies in Finnish Children." Journal of Medical Virology, vol. 83, no. 3, 2011, pp. 525-31.
Nurminen K, Blazevic V, Huhti L, et al. Prevalence of norovirus GII-4 antibodies in Finnish children. J Med Virol. 2011;83(3):525-31.
Nurminen, K., Blazevic, V., Huhti, L., Räsänen, S., Koho, T., Hytönen, V. P., & Vesikari, T. (2011). Prevalence of norovirus GII-4 antibodies in Finnish children. Journal of Medical Virology, 83(3), 525-31. https://doi.org/10.1002/jmv.21990
Nurminen K, et al. Prevalence of Norovirus GII-4 Antibodies in Finnish Children. J Med Virol. 2011;83(3):525-31. PubMed PMID: 21264875.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of norovirus GII-4 antibodies in Finnish children. AU - Nurminen,K, AU - Blazevic,V, AU - Huhti,L, AU - Räsänen,S, AU - Koho,T, AU - Hytönen,V P, AU - Vesikari,T, PY - 2011/1/26/entrez PY - 2011/1/26/pubmed PY - 2011/12/17/medline SP - 525 EP - 31 JF - Journal of medical virology JO - J Med Virol VL - 83 IS - 3 N2 - Noroviruses (NoVs) are the second most common cause of viral gastroenteritis after rotavirus in children. NoV genotype GII-4 has emerged as the major type not only in outbreaks of NoV gastroenteritis but also endemic gastroenteritis among infants and young children worldwide. Using baculovirus-insect cell system virus-like particles (VLPs) of NoV genotype GII-4 and an uncommon genotype GII-12 were produced. These VLPs were used in enzyme-linked immunosorbent assays (ELISA) for detection of NoV-specific immunoglobulin G (IgG) and IgA antibodies in 492 serum specimens from Finnish children 0-14 years of age collected between 2006 and 2008. NoV IgG antibody prevalence was 47.3% in the age group 7-23 months and increased up to 91.2% after the age of 5 years. Avidity of NoV IgG antibodies was low in the primary infections while high avidity antibodies were detected in the recurrent infections of the older children. In GII-4 infections, the homologous antibody response to GII-4 VLPs was stronger than to GII-12 VLPs but cross-reactivity between GII-4 and GII-12 was observed. Binding of GII-4 VLPs to a putative carbohydrate antigen receptor H-type 3 could be blocked by sera from children not infected with NoV during a waterborne outbreak of acute gastroenteritis. Therefore, protection against NoV infection correlated with strong blocking activity. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/21264875/Prevalence_of_norovirus_GII_4_antibodies_in_Finnish_children_ L2 - https://doi.org/10.1002/jmv.21990 DB - PRIME DP - Unbound Medicine ER -