Tags

Type your tag names separated by a space and hit enter

Phosphatidylinositol-4,5-bisphosphate influences Nt-Rac5-mediated cell expansion in pollen tubes of Nicotiana tabacum.
Plant J. 2011 Feb; 65(3):453-68.PJ

Abstract

The regulation of pollen tube growth by the phospholipid phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)) is not well understood. The Arabidopsis genome encodes two type A phosphatidylinositol-4-phosphate (PI4P) 5-kinases, PIP5K10 and PIP5K11, which are exclusively expressed in pollen and produce PtdIns(4,5)P(2) in vitro. Fluorescence-tagged PIP5K10 and PIP5K11 localized to lateral subapical plasma membrane microdomains in tobacco pollen tubes in a pattern closely resembling the distribution of PtdIns(4,5)P(2,) with the exception of notably weaker association at the extreme apex. Overexpression of PIP5K10 or PIP5K11 in tobacco pollen tubes resulted in severe tip swelling and altered actin fine structure similar to that reported for overexpression of tobacco Nt-Rac5, a monomeric GTPase known to regulate the actin cytoskeleton. Increased sensitivity of Arabidopsis pip5k10 pip5k11 double mutant pollen tubes to Latrunculin B (LatB) further supports a role for type A PI4P 5-kinases in controlling the actin cytoskeleton. Despite the disruption of both its type A PI4P 5-kinases, the pip5k10 pip5k11 double mutant was fertile, indicating that one of the remaining type B PI4P 5-kinase isoforms might be functionally redundant with PIP5K10 and PIP5K11. Antagonistic effects of PIP5K11 and the Nt-Rac5-specific guanine nucleotide dissociation inhibitor, Nt-RhoGDI2, on tip swelling observed in coexpression-titration experiments indicate a link between PtdIns(4,5)P(2) and Rac-signaling in pollen tubes. The data suggest that type A PI4P 5-kinases influence the actin cytoskeleton in pollen tubes in part by counteracting Nt-RhoGDI2, possibly contributing to the control of the pool of plasma membrane-associated Nt-Rac5.

Authors+Show Affiliations

Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University Göttingen, Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21265898

Citation

Ischebeck, Till, et al. "Phosphatidylinositol-4,5-bisphosphate Influences Nt-Rac5-mediated Cell Expansion in Pollen Tubes of Nicotiana Tabacum." The Plant Journal : for Cell and Molecular Biology, vol. 65, no. 3, 2011, pp. 453-68.
Ischebeck T, Stenzel I, Hempel F, et al. Phosphatidylinositol-4,5-bisphosphate influences Nt-Rac5-mediated cell expansion in pollen tubes of Nicotiana tabacum. Plant J. 2011;65(3):453-68.
Ischebeck, T., Stenzel, I., Hempel, F., Jin, X., Mosblech, A., & Heilmann, I. (2011). Phosphatidylinositol-4,5-bisphosphate influences Nt-Rac5-mediated cell expansion in pollen tubes of Nicotiana tabacum. The Plant Journal : for Cell and Molecular Biology, 65(3), 453-68. https://doi.org/10.1111/j.1365-313X.2010.04435.x
Ischebeck T, et al. Phosphatidylinositol-4,5-bisphosphate Influences Nt-Rac5-mediated Cell Expansion in Pollen Tubes of Nicotiana Tabacum. Plant J. 2011;65(3):453-68. PubMed PMID: 21265898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phosphatidylinositol-4,5-bisphosphate influences Nt-Rac5-mediated cell expansion in pollen tubes of Nicotiana tabacum. AU - Ischebeck,Till, AU - Stenzel,Irene, AU - Hempel,Franziska, AU - Jin,Xu, AU - Mosblech,Alina, AU - Heilmann,Ingo, Y1 - 2010/12/15/ PY - 2011/1/27/entrez PY - 2011/1/27/pubmed PY - 2012/4/4/medline SP - 453 EP - 68 JF - The Plant journal : for cell and molecular biology JO - Plant J. VL - 65 IS - 3 N2 - The regulation of pollen tube growth by the phospholipid phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)) is not well understood. The Arabidopsis genome encodes two type A phosphatidylinositol-4-phosphate (PI4P) 5-kinases, PIP5K10 and PIP5K11, which are exclusively expressed in pollen and produce PtdIns(4,5)P(2) in vitro. Fluorescence-tagged PIP5K10 and PIP5K11 localized to lateral subapical plasma membrane microdomains in tobacco pollen tubes in a pattern closely resembling the distribution of PtdIns(4,5)P(2,) with the exception of notably weaker association at the extreme apex. Overexpression of PIP5K10 or PIP5K11 in tobacco pollen tubes resulted in severe tip swelling and altered actin fine structure similar to that reported for overexpression of tobacco Nt-Rac5, a monomeric GTPase known to regulate the actin cytoskeleton. Increased sensitivity of Arabidopsis pip5k10 pip5k11 double mutant pollen tubes to Latrunculin B (LatB) further supports a role for type A PI4P 5-kinases in controlling the actin cytoskeleton. Despite the disruption of both its type A PI4P 5-kinases, the pip5k10 pip5k11 double mutant was fertile, indicating that one of the remaining type B PI4P 5-kinase isoforms might be functionally redundant with PIP5K10 and PIP5K11. Antagonistic effects of PIP5K11 and the Nt-Rac5-specific guanine nucleotide dissociation inhibitor, Nt-RhoGDI2, on tip swelling observed in coexpression-titration experiments indicate a link between PtdIns(4,5)P(2) and Rac-signaling in pollen tubes. The data suggest that type A PI4P 5-kinases influence the actin cytoskeleton in pollen tubes in part by counteracting Nt-RhoGDI2, possibly contributing to the control of the pool of plasma membrane-associated Nt-Rac5. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/21265898/Phosphatidylinositol_45_bisphosphate_influences_Nt_Rac5_mediated_cell_expansion_in_pollen_tubes_of_Nicotiana_tabacum_ L2 - https://doi.org/10.1111/j.1365-313X.2010.04435.x DB - PRIME DP - Unbound Medicine ER -