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A sensitive, label-free, aptamer-based biosensor using a gold nanoparticle-initiated chemiluminescence system.
Chemistry. 2011 Feb 01; 17(5):1642-8.C

Abstract

We report a label-free, aptamer-based chemiluminescent biosensor. The biosensor relies upon the catalytic activity of unmodified gold nanoparticles (AuNPs) on the luminol-H(2)O(2) chemiluminescence (CL) reaction, and the interaction of unmodified AuNPs with the aptamer. The unmodified AuNPs can effectively differentiate unstructured and folded aptamer. The binding of the aptamer with the target can induce the AuNP aggregation in the presence of 0.5 M NaCl, and after aggregation the catalytic activity of the AuNPs on the luminol-H(2)O(2) CL reaction is greatly enhanced. During the assay, no covalent functionalization of the AuNPs or aptamer is required. The detection limit of thrombin was estimated to be as low as 26 fM, and the sensitivity was more than 4 orders of magnitude better than that of known AuNP-based colorimetric methods for the detection of thrombin. This aptamer-based biosensor offers the advantages of being simple, cheap, rapid, and sensitive.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, P.R. China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21268167

Citation

Qi, Yingying, and Baoxin Li. "A Sensitive, Label-free, Aptamer-based Biosensor Using a Gold Nanoparticle-initiated Chemiluminescence System." Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 17, no. 5, 2011, pp. 1642-8.
Qi Y, Li B. A sensitive, label-free, aptamer-based biosensor using a gold nanoparticle-initiated chemiluminescence system. Chemistry. 2011;17(5):1642-8.
Qi, Y., & Li, B. (2011). A sensitive, label-free, aptamer-based biosensor using a gold nanoparticle-initiated chemiluminescence system. Chemistry (Weinheim an Der Bergstrasse, Germany), 17(5), 1642-8. https://doi.org/10.1002/chem.201001856
Qi Y, Li B. A Sensitive, Label-free, Aptamer-based Biosensor Using a Gold Nanoparticle-initiated Chemiluminescence System. Chemistry. 2011 Feb 1;17(5):1642-8. PubMed PMID: 21268167.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sensitive, label-free, aptamer-based biosensor using a gold nanoparticle-initiated chemiluminescence system. AU - Qi,Yingying, AU - Li,Baoxin, Y1 - 2010/12/07/ PY - 2010/07/01/received PY - 2011/1/27/entrez PY - 2011/1/27/pubmed PY - 2011/3/10/medline SP - 1642 EP - 8 JF - Chemistry (Weinheim an der Bergstrasse, Germany) JO - Chemistry VL - 17 IS - 5 N2 - We report a label-free, aptamer-based chemiluminescent biosensor. The biosensor relies upon the catalytic activity of unmodified gold nanoparticles (AuNPs) on the luminol-H(2)O(2) chemiluminescence (CL) reaction, and the interaction of unmodified AuNPs with the aptamer. The unmodified AuNPs can effectively differentiate unstructured and folded aptamer. The binding of the aptamer with the target can induce the AuNP aggregation in the presence of 0.5 M NaCl, and after aggregation the catalytic activity of the AuNPs on the luminol-H(2)O(2) CL reaction is greatly enhanced. During the assay, no covalent functionalization of the AuNPs or aptamer is required. The detection limit of thrombin was estimated to be as low as 26 fM, and the sensitivity was more than 4 orders of magnitude better than that of known AuNP-based colorimetric methods for the detection of thrombin. This aptamer-based biosensor offers the advantages of being simple, cheap, rapid, and sensitive. SN - 1521-3765 UR - https://www.unboundmedicine.com/medline/citation/21268167/A_sensitive_label_free_aptamer_based_biosensor_using_a_gold_nanoparticle_initiated_chemiluminescence_system_ L2 - https://doi.org/10.1002/chem.201001856 DB - PRIME DP - Unbound Medicine ER -