Tags

Type your tag names separated by a space and hit enter

Total oligomeric flavonoids of Cyperus rotundus ameliorates neurological deficits, excitotoxicity and behavioral alterations induced by cerebral ischemic-reperfusion injury in rats.
Brain Res Bull. 2011 Apr 05; 84(6):394-405.BR

Abstract

Interactions between neurons and astrocytes play a critical role in the central nervous system homeostasis. Cyperus rotundus (family: Cyperaceae), a traditional Indian medicinal herb, used as nervine tonic and nootropic in the Ayurvedic system of medicine. The present study was undertaken to investigate the neuroprotective effect of total oligomeric flavonoids (TOFs), prepared from C. rotundus, in rat model of cerebral ischemia and reperfusion. Male Sprague Dawley rats (290-340g) were subjected to middle cerebral artery occlusion (MCAO) for 2h and reperfusion for 70h. Experimental animals were divided into four groups: Group I - sham operated (n=7); Group II - vehicle treated ischemic-reperfusion (IR) (n=9), and Group III and IV - TOFs treated (100 and 200mg/kg body weight, p.o., respectively; n=7 in each group). Vehicle or TOFs were pretreated for four days before the induction of ischemia and continued for next three days after the ischemia i.e. treatment was scheduled totally for a period of 7 days. MCAO surgery was performed on day 4, 1h after TOFs administration. Neuroprotective effect of TOFs was substantiated in terms of neurological deficits, excitotoxicity (glutamate, glutamine synthetase and Na(+)K(+)ATPase levels), oxidative stress (malondialdehyde, super oxide dismutase, and glutathione) and neurobehavioral functions in the experimental animals. TOFs decreased glutamate, glutamine synthetase (GS) and increased Na(+)K(+)ATPase activity in a dose dependent manner when compared to the IR rats. Treatment with TOFs significantly reduced the neurological deficits and reversed the anxiogenic behavior in rats. Further, it also significantly decreased MDA and increased superoxide dismutase (SOD) and glutathione content in brains of experimental rats. Histopathological examination using cresyl violet staining revealed the attenuation of neuronal loss by TOFs in stroke rats. The present study demonstrates the unswerving involvement of TOFs on ischemia-reperfusion triggered biochemical alterations in MCAO/R rats. Hence, TOFs might be an attractive candidate for further studies in the development of new drugs for cerebral stroke treatment.

Authors+Show Affiliations

Centre for Toxicology and Developmental Research (CEFT), Sri Ramachandra University, Chennai, Tamilnadu, India. ceftpublications@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21272618

Citation

Sunil, A G., et al. "Total Oligomeric Flavonoids of Cyperus Rotundus Ameliorates Neurological Deficits, Excitotoxicity and Behavioral Alterations Induced By Cerebral Ischemic-reperfusion Injury in Rats." Brain Research Bulletin, vol. 84, no. 6, 2011, pp. 394-405.
Sunil AG, Kesavanarayanan KS, Kalaivani P, et al. Total oligomeric flavonoids of Cyperus rotundus ameliorates neurological deficits, excitotoxicity and behavioral alterations induced by cerebral ischemic-reperfusion injury in rats. Brain Res Bull. 2011;84(6):394-405.
Sunil, A. G., Kesavanarayanan, K. S., Kalaivani, P., Sathiya, S., Ranju, V., Priya, R. J., Pramila, B., Paul, F. D., Venkhatesh, J., & Babu, C. S. (2011). Total oligomeric flavonoids of Cyperus rotundus ameliorates neurological deficits, excitotoxicity and behavioral alterations induced by cerebral ischemic-reperfusion injury in rats. Brain Research Bulletin, 84(6), 394-405. https://doi.org/10.1016/j.brainresbull.2011.01.008
Sunil AG, et al. Total Oligomeric Flavonoids of Cyperus Rotundus Ameliorates Neurological Deficits, Excitotoxicity and Behavioral Alterations Induced By Cerebral Ischemic-reperfusion Injury in Rats. Brain Res Bull. 2011 Apr 5;84(6):394-405. PubMed PMID: 21272618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Total oligomeric flavonoids of Cyperus rotundus ameliorates neurological deficits, excitotoxicity and behavioral alterations induced by cerebral ischemic-reperfusion injury in rats. AU - Sunil,A G, AU - Kesavanarayanan,K S, AU - Kalaivani,P, AU - Sathiya,S, AU - Ranju,V, AU - Priya,R Jyothi, AU - Pramila,B, AU - Paul,F D Solomon, AU - Venkhatesh,J, AU - Babu,C Saravana, Y1 - 2011/01/25/ PY - 2010/09/29/received PY - 2011/01/10/revised PY - 2011/01/18/accepted PY - 2011/1/29/entrez PY - 2011/1/29/pubmed PY - 2011/7/19/medline SP - 394 EP - 405 JF - Brain research bulletin JO - Brain Res. Bull. VL - 84 IS - 6 N2 - Interactions between neurons and astrocytes play a critical role in the central nervous system homeostasis. Cyperus rotundus (family: Cyperaceae), a traditional Indian medicinal herb, used as nervine tonic and nootropic in the Ayurvedic system of medicine. The present study was undertaken to investigate the neuroprotective effect of total oligomeric flavonoids (TOFs), prepared from C. rotundus, in rat model of cerebral ischemia and reperfusion. Male Sprague Dawley rats (290-340g) were subjected to middle cerebral artery occlusion (MCAO) for 2h and reperfusion for 70h. Experimental animals were divided into four groups: Group I - sham operated (n=7); Group II - vehicle treated ischemic-reperfusion (IR) (n=9), and Group III and IV - TOFs treated (100 and 200mg/kg body weight, p.o., respectively; n=7 in each group). Vehicle or TOFs were pretreated for four days before the induction of ischemia and continued for next three days after the ischemia i.e. treatment was scheduled totally for a period of 7 days. MCAO surgery was performed on day 4, 1h after TOFs administration. Neuroprotective effect of TOFs was substantiated in terms of neurological deficits, excitotoxicity (glutamate, glutamine synthetase and Na(+)K(+)ATPase levels), oxidative stress (malondialdehyde, super oxide dismutase, and glutathione) and neurobehavioral functions in the experimental animals. TOFs decreased glutamate, glutamine synthetase (GS) and increased Na(+)K(+)ATPase activity in a dose dependent manner when compared to the IR rats. Treatment with TOFs significantly reduced the neurological deficits and reversed the anxiogenic behavior in rats. Further, it also significantly decreased MDA and increased superoxide dismutase (SOD) and glutathione content in brains of experimental rats. Histopathological examination using cresyl violet staining revealed the attenuation of neuronal loss by TOFs in stroke rats. The present study demonstrates the unswerving involvement of TOFs on ischemia-reperfusion triggered biochemical alterations in MCAO/R rats. Hence, TOFs might be an attractive candidate for further studies in the development of new drugs for cerebral stroke treatment. SN - 1873-2747 UR - https://www.unboundmedicine.com/medline/citation/21272618/Total_oligomeric_flavonoids_of_Cyperus_rotundus_ameliorates_neurological_deficits_excitotoxicity_and_behavioral_alterations_induced_by_cerebral_ischemic_reperfusion_injury_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0361-9230(11)00011-6 DB - PRIME DP - Unbound Medicine ER -