Tags

Type your tag names separated by a space and hit enter

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with magnetic beads for detecting serum protein biomarkers in parkinson's disease.
Eur Neurol. 2011; 65(2):105-11.EN

Abstract

BACKGROUND

Biomarkers for neurodegenerative diseases are essential to facilitate disease diagnosis. Application of proteomics has greatly hastened the search for novel biomarkers. In this study, new potential biomarkers were discovered, and a diagnostic pattern was established for idiopathic Parkinson's disease (PD) by using proteomic technology.

METHODS

Serum proteins from PD patients and controls were captured by magnetic bead-based weak cation exchange. The molecular weight of the proteins in bead-binding fraction was detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Biomarker Wizard 3.1 and Biomarker Patterns Software were used for data analysis and constructing a model of biomarkers. A blinded testing set was used to validate the model.

RESULTS

A total of 17 discriminating m/z peaks related to PD were identified. The model based on the 5 biomarkers generated an excellent separation between PD and healthy controls with 98.36% for the sensitivity and 83.05% for the specificity. Blind test data demonstrated the model could recognize patients with PD with a sensitivity of 85.0% and a specificity of 70.0%.

CONCLUSIONS

The preliminary data suggested a potential application of MALDI-TOF-MS combined with magnetic beads. The model comprising 5 promising biomarkers can differentiate individuals with PD and the healthy subjects precisely.

Links

Publisher Full Text

Authors+Show Affiliations

Li YH
Beijing Institute of Geriatrics, Xuanwu Hospital of Capital University of Medical Sciences, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, China. liyaohua1962@yahoo.com.cn
Wang J
No affiliation info available
Zheng XL
No affiliation info available
Zhang YL
No affiliation info available
Li X
No affiliation info available
Yu S
No affiliation info available
He X
No affiliation info available
Chan P
No affiliation info available

MeSH

AdultAgedBiomarkersBlood ProteinsFemaleHumansImmunomagnetic SeparationMaleMiddle AgedParkinson DiseaseProteomicsSensitivity and SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationYoung Adult

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21273779

Citation

Li, Yao-Hua, et al. "Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Combined With Magnetic Beads for Detecting Serum Protein Biomarkers in Parkinson's Disease." European Neurology, vol. 65, no. 2, 2011, pp. 105-11.
Li YH, Wang J, Zheng XL, et al. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with magnetic beads for detecting serum protein biomarkers in parkinson's disease. Eur Neurol. 2011;65(2):105-11.
Li, Y. H., Wang, J., Zheng, X. L., Zhang, Y. L., Li, X., Yu, S., He, X., & Chan, P. (2011). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with magnetic beads for detecting serum protein biomarkers in parkinson's disease. European Neurology, 65(2), 105-11. https://doi.org/10.1159/000323427
Li YH, et al. Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Combined With Magnetic Beads for Detecting Serum Protein Biomarkers in Parkinson's Disease. Eur Neurol. 2011;65(2):105-11. PubMed PMID: 21273779.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with magnetic beads for detecting serum protein biomarkers in parkinson's disease. AU - Li,Yao-Hua, AU - Wang,Jian, AU - Zheng,Xiao-Li, AU - Zhang,Yan-Li, AU - Li,Xin, AU - Yu,Shun, AU - He,Xin, AU - Chan,Piu, Y1 - 2011/01/27/ PY - 2010/11/03/received PY - 2010/12/05/accepted PY - 2011/1/29/entrez PY - 2011/1/29/pubmed PY - 2011/6/15/medline SP - 105 EP - 11 JF - European neurology JO - Eur Neurol VL - 65 IS - 2 N2 - BACKGROUND: Biomarkers for neurodegenerative diseases are essential to facilitate disease diagnosis. Application of proteomics has greatly hastened the search for novel biomarkers. In this study, new potential biomarkers were discovered, and a diagnostic pattern was established for idiopathic Parkinson's disease (PD) by using proteomic technology. METHODS: Serum proteins from PD patients and controls were captured by magnetic bead-based weak cation exchange. The molecular weight of the proteins in bead-binding fraction was detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Biomarker Wizard 3.1 and Biomarker Patterns Software were used for data analysis and constructing a model of biomarkers. A blinded testing set was used to validate the model. RESULTS: A total of 17 discriminating m/z peaks related to PD were identified. The model based on the 5 biomarkers generated an excellent separation between PD and healthy controls with 98.36% for the sensitivity and 83.05% for the specificity. Blind test data demonstrated the model could recognize patients with PD with a sensitivity of 85.0% and a specificity of 70.0%. CONCLUSIONS: The preliminary data suggested a potential application of MALDI-TOF-MS combined with magnetic beads. The model comprising 5 promising biomarkers can differentiate individuals with PD and the healthy subjects precisely. SN - 1421-9913 UR - https://www.unboundmedicine.com/medline/citation/21273779/Matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_combined_with_magnetic_beads_for_detecting_serum_protein_biomarkers_in_parkinson's_disease_ L2 - https://www.karger.com?DOI=10.1159/000323427 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓6]

Related Citations

More