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Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management.
J Proteomics. 2011 Apr 01; 74(4):510-27.JP

Abstract

We describe two geographically differentiated venom phenotypes across the wide distribution range of Bothrops atrox, from the Colombian Magdalena Medio Valley through Puerto Ayacucho and El Paují, in the Venezuelan States of Amazonas and Orinoquia, respectively, and São Bento in the Brazilian State of Maranhão. Colombian and Venezuelan venoms show an ontogenetic toxin profile phenotype whereas Brazilian venoms exhibit paedomorphic phenotypes. Venoms from each of the 16 localities sampled contain both population-specific toxins and proteins shared by neighboring B. atrox populations. Mapping the molecular similarity between conspecific populations onto a physical map of B. atrox range provides clues for tracing dispersal routes that account for the current biogeographic distribution of the species. The proteomic pattern is consistent with a model of southeast and southwest dispersal and allopatric fragmentation northern of the Amazon Basin, and trans-Amazonian expansion through the Andean Corridor and across the Amazon river between Monte Alegre and Santarém. An antivenomic approach applied to assess the efficacy towards B. atrox venoms of two antivenoms raised in Costa Rica and Brazil using Bothrops venoms different than B. atrox in the immunization mixtures showed that both antivenoms immunodepleted very efficiently the major toxins (PIII-SVMPs, serine proteinases, CRISP, LAO) of paedomorphic venoms from Puerto Ayacucho (Venezuelan Amazonia) through São Bento, but had impaired reactivity towards PLA(2) and P-I SVMP molecules abundantly present in ontogenetic venoms. The degree of immunodepletion achieved suggests that each of these antivenoms may be effective against envenomations by paedomorphic, and some ontogenetic, B. atrox venoms.

Authors+Show Affiliations

Instituto de Biomedicina de Valencia, C.S.I.C., Jaume Roig 11, 46010 Valencia, Spain. jcalvete@ibv.csic.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21278006

Citation

Calvete, Juan J., et al. "Snake Population Venomics and Antivenomics of Bothrops Atrox: Paedomorphism Along Its Transamazonian Dispersal and Implications of Geographic Venom Variability On Snakebite Management." Journal of Proteomics, vol. 74, no. 4, 2011, pp. 510-27.
Calvete JJ, Sanz L, Pérez A, et al. Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management. J Proteomics. 2011;74(4):510-27.
Calvete, J. J., Sanz, L., Pérez, A., Borges, A., Vargas, A. M., Lomonte, B., Angulo, Y., Gutiérrez, J. M., Chalkidis, H. M., Mourão, R. H., Furtado, M. F., & Moura-Da-Silva, A. M. (2011). Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management. Journal of Proteomics, 74(4), 510-27. https://doi.org/10.1016/j.jprot.2011.01.003
Calvete JJ, et al. Snake Population Venomics and Antivenomics of Bothrops Atrox: Paedomorphism Along Its Transamazonian Dispersal and Implications of Geographic Venom Variability On Snakebite Management. J Proteomics. 2011 Apr 1;74(4):510-27. PubMed PMID: 21278006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management. AU - Calvete,Juan J, AU - Sanz,Libia, AU - Pérez,Alicia, AU - Borges,Adolfo, AU - Vargas,Alba M, AU - Lomonte,Bruno, AU - Angulo,Yamileth, AU - Gutiérrez,José María, AU - Chalkidis,Hipócrates M, AU - Mourão,Rosa H V, AU - Furtado,M Fatima D, AU - Moura-Da-Silva,Ana M, Y1 - 2011/01/26/ PY - 2010/12/10/received PY - 2010/12/28/revised PY - 2011/01/10/accepted PY - 2011/2/1/entrez PY - 2011/2/1/pubmed PY - 2011/6/22/medline SP - 510 EP - 27 JF - Journal of proteomics JO - J Proteomics VL - 74 IS - 4 N2 - We describe two geographically differentiated venom phenotypes across the wide distribution range of Bothrops atrox, from the Colombian Magdalena Medio Valley through Puerto Ayacucho and El Paují, in the Venezuelan States of Amazonas and Orinoquia, respectively, and São Bento in the Brazilian State of Maranhão. Colombian and Venezuelan venoms show an ontogenetic toxin profile phenotype whereas Brazilian venoms exhibit paedomorphic phenotypes. Venoms from each of the 16 localities sampled contain both population-specific toxins and proteins shared by neighboring B. atrox populations. Mapping the molecular similarity between conspecific populations onto a physical map of B. atrox range provides clues for tracing dispersal routes that account for the current biogeographic distribution of the species. The proteomic pattern is consistent with a model of southeast and southwest dispersal and allopatric fragmentation northern of the Amazon Basin, and trans-Amazonian expansion through the Andean Corridor and across the Amazon river between Monte Alegre and Santarém. An antivenomic approach applied to assess the efficacy towards B. atrox venoms of two antivenoms raised in Costa Rica and Brazil using Bothrops venoms different than B. atrox in the immunization mixtures showed that both antivenoms immunodepleted very efficiently the major toxins (PIII-SVMPs, serine proteinases, CRISP, LAO) of paedomorphic venoms from Puerto Ayacucho (Venezuelan Amazonia) through São Bento, but had impaired reactivity towards PLA(2) and P-I SVMP molecules abundantly present in ontogenetic venoms. The degree of immunodepletion achieved suggests that each of these antivenoms may be effective against envenomations by paedomorphic, and some ontogenetic, B. atrox venoms. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/21278006/Snake_population_venomics_and_antivenomics_of_Bothrops_atrox:_Paedomorphism_along_its_transamazonian_dispersal_and_implications_of_geographic_venom_variability_on_snakebite_management_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(11)00020-0 DB - PRIME DP - Unbound Medicine ER -