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[Evaluation of an IgG avidity commercial test: contribution to diagnosis of primary infection caused by Toxoplasma gondii].
Rev Chilena Infectol 2010; 27(6):499-504RC

Abstract

INTRODUCTION

Toxoplasmosis (T) is a major chronic parasitic infection in immunocompromised patients and pregnant women. It is important to discriminate between acute phase (AT) and chronic phase (CT). Diagnosis is serological in immunocompetent patients (concentration of IgG and IgM).

OBJECTIVE

To evaluate the utility of an IgG avidity test (A-IgG) to identify the acute and chronic stage. Avidity is the strength of affinity between a specific immunoglobulin and the protein antigenic epitope of the infecting agent, an affinity that increases over time.

PATIENTS AND METHODS

We used a qualitative kit that measures the avidity of IgG, discriminating the two phases. In 35 patients with clinical diagnosis of AT and/or CT, IgG, IgM and IgG A (VIDAS®) were performed.

RESULTS

Patients with AT were positive for IgM and IgG, but presented weak avidity. In the 21 cases with CT, 52% (n: 11) were IgM positive and 100% (n: 21) had positive IgG with strong avidity.

DISCUSSION

The results confirm that the test of A-IgG may be useful in the diagnosis of AT, and has 100% concordance with reference test (qualitative IgM + quantitative IgG). The result is available within 24 hrs, and may be useful in diagnosis of AT in pregnant women.

Authors+Show Affiliations

Laboratorio de Microbiología, Servicio de Laboratorios Clínicos, Pontificia Universidad Católica de Chile, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

21279286

Citation

Canales R, Marilena, et al. "[Evaluation of an IgG Avidity Commercial Test: Contribution to Diagnosis of Primary Infection Caused By Toxoplasma Gondii]." Revista Chilena De Infectologia : Organo Oficial De La Sociedad Chilena De Infectologia, vol. 27, no. 6, 2010, pp. 499-504.
Canales R M, Navia G F, Torres H M, et al. [Evaluation of an IgG avidity commercial test: contribution to diagnosis of primary infection caused by Toxoplasma gondii]. Rev Chilena Infectol. 2010;27(6):499-504.
Canales R, M., Navia G, F., Torres H, M., Concha C, M., Guzmán D, A. M., Pérez C, C., & García C, P. (2010). [Evaluation of an IgG avidity commercial test: contribution to diagnosis of primary infection caused by Toxoplasma gondii]. Revista Chilena De Infectologia : Organo Oficial De La Sociedad Chilena De Infectologia, 27(6), pp. 499-504. doi:/S0716-10182010000700002.
Canales R M, et al. [Evaluation of an IgG Avidity Commercial Test: Contribution to Diagnosis of Primary Infection Caused By Toxoplasma Gondii]. Rev Chilena Infectol. 2010;27(6):499-504. PubMed PMID: 21279286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Evaluation of an IgG avidity commercial test: contribution to diagnosis of primary infection caused by Toxoplasma gondii]. AU - Canales R,Marilena, AU - Navia G,Felipe, AU - Torres H,Marisa, AU - Concha C,Mónica, AU - Guzmán D,Ana M, AU - Pérez C,Carlos, AU - García C,Patricia, Y1 - 2011/01/07/ PY - 2011/2/1/entrez PY - 2011/2/1/pubmed PY - 2011/7/27/medline SP - 499 EP - 504 JF - Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia JO - Rev Chilena Infectol VL - 27 IS - 6 N2 - INTRODUCTION: Toxoplasmosis (T) is a major chronic parasitic infection in immunocompromised patients and pregnant women. It is important to discriminate between acute phase (AT) and chronic phase (CT). Diagnosis is serological in immunocompetent patients (concentration of IgG and IgM). OBJECTIVE: To evaluate the utility of an IgG avidity test (A-IgG) to identify the acute and chronic stage. Avidity is the strength of affinity between a specific immunoglobulin and the protein antigenic epitope of the infecting agent, an affinity that increases over time. PATIENTS AND METHODS: We used a qualitative kit that measures the avidity of IgG, discriminating the two phases. In 35 patients with clinical diagnosis of AT and/or CT, IgG, IgM and IgG A (VIDAS®) were performed. RESULTS: Patients with AT were positive for IgM and IgG, but presented weak avidity. In the 21 cases with CT, 52% (n: 11) were IgM positive and 100% (n: 21) had positive IgG with strong avidity. DISCUSSION: The results confirm that the test of A-IgG may be useful in the diagnosis of AT, and has 100% concordance with reference test (qualitative IgM + quantitative IgG). The result is available within 24 hrs, and may be useful in diagnosis of AT in pregnant women. SN - 0716-1018 UR - https://www.unboundmedicine.com/medline/citation/21279286/[Evaluation_of_an_IgG_avidity_commercial_test:_contribution_to_diagnosis_of_primary_infection_caused_by_Toxoplasma_gondii]_ L2 - http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182010000700002&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -