Tags

Type your tag names separated by a space and hit enter

Acute toxicity, biochemical and gene expression responses of the earthworm Eisenia fetida exposed to polycyclic musks.
Chemosphere. 2011 May; 83(8):1147-54.C

Abstract

AHTN (Tonalide) and HHCB (galaxolide) are recognized as ubiquitous contaminants in soil and have potential adverse impacts on soil organisms. The aim of this study is to investigate the effects of AHTN and HHCB on the earthworm (Eisenia fetida) as an important soil animal with attention to the acute toxicity, biochemical and transcriptional changes of representative antioxidant enzymatic (SOD, CAT) and stress-response gene (Hsp70). The 48 h-LC(50) value was 20.76 μg cm(-2) for AHTN and 11.87 μg cm(-2) for HHCB respectively in the acute lethal studies. The time-dependent elevation in the level of malondialdehyde (MDA) suggests that reactive oxygen species (ROS)-induced cellular oxidative injury of E. fetida might be one of the main toxic effects of AHTN and HHCB. SOD and CAT were both up-regulated at low exposure dose (0.6 μg cm(-2) AHTN and 0.3 μg cm(-2) HHCB) during 48 h testing period, which protected earthworms from oxidative stresses. However, the down-regulation of SOD and CAT after 48 h exposure to high dose contaminants might be caused by the extreme oxidative stress levels (maximum up-regulation 1.70-fold and 1.40-fold for MDA levels at 6.0 μg cm(-2) AHTN and 3.0 μg cm(-2) HHCB compared to the controls, respectively). The Hsp70 gene expression did not show variation during 48 h, except that it had a significant down-regulation (P<0.05) after 48 h of exposure to high doses of contaminants. These results showed that the dermal contact of AHTN and HHCB could result in pronounced biochemical and physiological responses to earthworms, and the transcriptional level changes in antioxidant genes could be potential molecular biomarkers for the stress of the pollutants.

Authors+Show Affiliations

Key Laboratory of Pollution Processes and Environmental Criteria at Ministry of Education, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21281957

Citation

Chen, Chun, et al. "Acute Toxicity, Biochemical and Gene Expression Responses of the Earthworm Eisenia Fetida Exposed to Polycyclic Musks." Chemosphere, vol. 83, no. 8, 2011, pp. 1147-54.
Chen C, Zhou Q, Liu S, et al. Acute toxicity, biochemical and gene expression responses of the earthworm Eisenia fetida exposed to polycyclic musks. Chemosphere. 2011;83(8):1147-54.
Chen, C., Zhou, Q., Liu, S., & Xiu, Z. (2011). Acute toxicity, biochemical and gene expression responses of the earthworm Eisenia fetida exposed to polycyclic musks. Chemosphere, 83(8), 1147-54. https://doi.org/10.1016/j.chemosphere.2011.01.006
Chen C, et al. Acute Toxicity, Biochemical and Gene Expression Responses of the Earthworm Eisenia Fetida Exposed to Polycyclic Musks. Chemosphere. 2011;83(8):1147-54. PubMed PMID: 21281957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute toxicity, biochemical and gene expression responses of the earthworm Eisenia fetida exposed to polycyclic musks. AU - Chen,Chun, AU - Zhou,Qixing, AU - Liu,Shuo, AU - Xiu,Zongming, Y1 - 2011/02/01/ PY - 2010/09/15/received PY - 2010/12/31/revised PY - 2011/01/02/accepted PY - 2011/2/2/entrez PY - 2011/2/2/pubmed PY - 2011/7/1/medline SP - 1147 EP - 54 JF - Chemosphere JO - Chemosphere VL - 83 IS - 8 N2 - AHTN (Tonalide) and HHCB (galaxolide) are recognized as ubiquitous contaminants in soil and have potential adverse impacts on soil organisms. The aim of this study is to investigate the effects of AHTN and HHCB on the earthworm (Eisenia fetida) as an important soil animal with attention to the acute toxicity, biochemical and transcriptional changes of representative antioxidant enzymatic (SOD, CAT) and stress-response gene (Hsp70). The 48 h-LC(50) value was 20.76 μg cm(-2) for AHTN and 11.87 μg cm(-2) for HHCB respectively in the acute lethal studies. The time-dependent elevation in the level of malondialdehyde (MDA) suggests that reactive oxygen species (ROS)-induced cellular oxidative injury of E. fetida might be one of the main toxic effects of AHTN and HHCB. SOD and CAT were both up-regulated at low exposure dose (0.6 μg cm(-2) AHTN and 0.3 μg cm(-2) HHCB) during 48 h testing period, which protected earthworms from oxidative stresses. However, the down-regulation of SOD and CAT after 48 h exposure to high dose contaminants might be caused by the extreme oxidative stress levels (maximum up-regulation 1.70-fold and 1.40-fold for MDA levels at 6.0 μg cm(-2) AHTN and 3.0 μg cm(-2) HHCB compared to the controls, respectively). The Hsp70 gene expression did not show variation during 48 h, except that it had a significant down-regulation (P<0.05) after 48 h of exposure to high doses of contaminants. These results showed that the dermal contact of AHTN and HHCB could result in pronounced biochemical and physiological responses to earthworms, and the transcriptional level changes in antioxidant genes could be potential molecular biomarkers for the stress of the pollutants. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/21281957/Acute_toxicity_biochemical_and_gene_expression_responses_of_the_earthworm_Eisenia_fetida_exposed_to_polycyclic_musks_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(11)00008-7 DB - PRIME DP - Unbound Medicine ER -