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Genetic suppression analysis in novel vacuolar processing enzymes reveals their roles in controlling sugar accumulation in tomato fruits.
J Exp Bot. 2011 May; 62(8):2773-86.JE

Abstract

In plant cells, many vacuolar proteins are synthesized as precursors in the endoplasmic reticulum and are subsequently transported to the vacuole. These precursors are subject to post-translational modifications to allow the active mature forms to be produced. Vacuolar processing enzyme (VPE) has been identified as a family of cysteine proteases involved in protein maturation in the vacuole. In this study, novel VPE genes were isolated from tomato (Solanum lycopersicum), and they were designated SlVPE1-SlVPE5. Phylogenic analysis suggested that SlVPE1 and SlVPE2 were categorized as the seed coat type, SlVPE4 was categorized as the seed type, and both SlVPE3 and SlVPE5 were categorized as the vegetative type. Expression analysis demonstrated that these genes were expressed during fruit development, and that their expression profiles agreed with this classification. High VPE enzyme activity was observed during tomato fruit development; the enzyme activity was correlated with the SlVPE mRNA levels, indicating that the SlVPE encoded active VPE proteins. The total sugar content was higher in RNA interference (RNAi) lines compared with the control plants, suggesting negative roles for SlVPE in sugar accumulation. The quantitative expression analysis of each SlVPE gene in the RNAi lines suggested that the suppression of SlVPE5 probably had the strongest effect on the sugar accumulation observed. The suppression of SlVPE did not influence the total amino acid content, suggesting that the molecular targets of SlVPE were mainly involved in sugar accumulation.

Authors+Show Affiliations

Graduate School of Environmental Sciences, Gene Research Center, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8572. Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21282322

Citation

Ariizumi, Tohru, et al. "Genetic Suppression Analysis in Novel Vacuolar Processing Enzymes Reveals Their Roles in Controlling Sugar Accumulation in Tomato Fruits." Journal of Experimental Botany, vol. 62, no. 8, 2011, pp. 2773-86.
Ariizumi T, Higuchi K, Arakaki S, et al. Genetic suppression analysis in novel vacuolar processing enzymes reveals their roles in controlling sugar accumulation in tomato fruits. J Exp Bot. 2011;62(8):2773-86.
Ariizumi, T., Higuchi, K., Arakaki, S., Sano, T., Asamizu, E., & Ezura, H. (2011). Genetic suppression analysis in novel vacuolar processing enzymes reveals their roles in controlling sugar accumulation in tomato fruits. Journal of Experimental Botany, 62(8), 2773-86. https://doi.org/10.1093/jxb/erq451
Ariizumi T, et al. Genetic Suppression Analysis in Novel Vacuolar Processing Enzymes Reveals Their Roles in Controlling Sugar Accumulation in Tomato Fruits. J Exp Bot. 2011;62(8):2773-86. PubMed PMID: 21282322.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic suppression analysis in novel vacuolar processing enzymes reveals their roles in controlling sugar accumulation in tomato fruits. AU - Ariizumi,Tohru, AU - Higuchi,Kenji, AU - Arakaki,Shoko, AU - Sano,Tsunenori, AU - Asamizu,Erika, AU - Ezura,Hiroshi, Y1 - 2011/01/31/ PY - 2011/2/2/entrez PY - 2011/2/2/pubmed PY - 2011/9/7/medline SP - 2773 EP - 86 JF - Journal of experimental botany JO - J Exp Bot VL - 62 IS - 8 N2 - In plant cells, many vacuolar proteins are synthesized as precursors in the endoplasmic reticulum and are subsequently transported to the vacuole. These precursors are subject to post-translational modifications to allow the active mature forms to be produced. Vacuolar processing enzyme (VPE) has been identified as a family of cysteine proteases involved in protein maturation in the vacuole. In this study, novel VPE genes were isolated from tomato (Solanum lycopersicum), and they were designated SlVPE1-SlVPE5. Phylogenic analysis suggested that SlVPE1 and SlVPE2 were categorized as the seed coat type, SlVPE4 was categorized as the seed type, and both SlVPE3 and SlVPE5 were categorized as the vegetative type. Expression analysis demonstrated that these genes were expressed during fruit development, and that their expression profiles agreed with this classification. High VPE enzyme activity was observed during tomato fruit development; the enzyme activity was correlated with the SlVPE mRNA levels, indicating that the SlVPE encoded active VPE proteins. The total sugar content was higher in RNA interference (RNAi) lines compared with the control plants, suggesting negative roles for SlVPE in sugar accumulation. The quantitative expression analysis of each SlVPE gene in the RNAi lines suggested that the suppression of SlVPE5 probably had the strongest effect on the sugar accumulation observed. The suppression of SlVPE did not influence the total amino acid content, suggesting that the molecular targets of SlVPE were mainly involved in sugar accumulation. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/21282322/Genetic_suppression_analysis_in_novel_vacuolar_processing_enzymes_reveals_their_roles_in_controlling_sugar_accumulation_in_tomato_fruits_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erq451 DB - PRIME DP - Unbound Medicine ER -