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Verifying of participation of nitric oxide in morphine place conditioning in the rat medial septum using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d).
Iran Biomed J. 2010 Oct; 14(4):150-7.IB

Abstract

BACKGROUND

Role of nitric oxide (NO) in morphine-induced conditioned place preference (CPP) has already been proposed in the rat medial septum (MS), but no molecular evidence has been provided to clear this fact.

METHODS

Effects of intraseptal injections of L-arginine and/or NG-nitro-L-arginine methyl ester (L-NAME) on morphine place conditioning in Wistar rats were examined. Morphine (2.5-7.5 mg/kg) was injected s.c. using a three-day schedule of an unbiased place preference. All of the brain samples were examined histochemically by nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d), the main marker for NO activation.

RESULTS

Morphine induced a significant CPP in the rats. Single injections of L-arginine or L-NAME (0.3, 1.0 and 3.0 µg/rat) did not induce CPP. In addition, co-administration of morphine (5.0 mg/kg) with L-arginine or L-NAME (0.3, 1.0 and 3.0 µg/rat) did not affect morphine response. However, administration of L-arginine (0.3, 1.0 and 3.0 µg/rat) prior to morphine conditioning testing enhanced the expression of morphine response. Moreover, pre-injection of L-NAME (0.3, 1.0 and 3.0 µg/rat) to L-arginine (0.3 µg/rat) did not reverse the response to the agent. The expression of NADPH-d was observed in the rat brain samples treated by L-arginine. A decreased expression of NADPH-d was also observed in rats pre-injected by L-NAME.

CONCLUSION

This finding strongly suggests that NO system in the rat MS has an impact on the expression of morphine rewarding, and that the NO participates in place conditioning induced of morphine.

Authors+Show Affiliations

Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran. karami@shahed.ac.irNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21283257

Citation

Karami, Manizheh, et al. "Verifying of Participation of Nitric Oxide in Morphine Place Conditioning in the Rat Medial Septum Using Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d)." Iranian Biomedical Journal, vol. 14, no. 4, 2010, pp. 150-7.
Karami M, Karimian Azimi M, Zarrindast MR, et al. Verifying of participation of nitric oxide in morphine place conditioning in the rat medial septum using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d). Iran Biomed J. 2010;14(4):150-7.
Karami, M., Karimian Azimi, M., Zarrindast, M. R., & Khalaji, Z. (2010). Verifying of participation of nitric oxide in morphine place conditioning in the rat medial septum using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d). Iranian Biomedical Journal, 14(4), 150-7.
Karami M, et al. Verifying of Participation of Nitric Oxide in Morphine Place Conditioning in the Rat Medial Septum Using Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d). Iran Biomed J. 2010;14(4):150-7. PubMed PMID: 21283257.
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TY - JOUR T1 - Verifying of participation of nitric oxide in morphine place conditioning in the rat medial septum using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d). AU - Karami,Manizheh, AU - Karimian Azimi,Mohsen, AU - Zarrindast,Mohammad Reza, AU - Khalaji,Zeinab, PY - 2011/2/2/entrez PY - 2011/2/2/pubmed PY - 2011/5/27/medline SP - 150 EP - 7 JF - Iranian biomedical journal JO - Iran Biomed J VL - 14 IS - 4 N2 - BACKGROUND: Role of nitric oxide (NO) in morphine-induced conditioned place preference (CPP) has already been proposed in the rat medial septum (MS), but no molecular evidence has been provided to clear this fact. METHODS: Effects of intraseptal injections of L-arginine and/or NG-nitro-L-arginine methyl ester (L-NAME) on morphine place conditioning in Wistar rats were examined. Morphine (2.5-7.5 mg/kg) was injected s.c. using a three-day schedule of an unbiased place preference. All of the brain samples were examined histochemically by nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d), the main marker for NO activation. RESULTS: Morphine induced a significant CPP in the rats. Single injections of L-arginine or L-NAME (0.3, 1.0 and 3.0 µg/rat) did not induce CPP. In addition, co-administration of morphine (5.0 mg/kg) with L-arginine or L-NAME (0.3, 1.0 and 3.0 µg/rat) did not affect morphine response. However, administration of L-arginine (0.3, 1.0 and 3.0 µg/rat) prior to morphine conditioning testing enhanced the expression of morphine response. Moreover, pre-injection of L-NAME (0.3, 1.0 and 3.0 µg/rat) to L-arginine (0.3 µg/rat) did not reverse the response to the agent. The expression of NADPH-d was observed in the rat brain samples treated by L-arginine. A decreased expression of NADPH-d was also observed in rats pre-injected by L-NAME. CONCLUSION: This finding strongly suggests that NO system in the rat MS has an impact on the expression of morphine rewarding, and that the NO participates in place conditioning induced of morphine. SN - 2008-823X UR - https://www.unboundmedicine.com/medline/citation/21283257/Verifying_of_participation_of_nitric_oxide_in_morphine_place_conditioning_in_the_rat_medial_septum_using_nicotinamide_adenine_dinucleotide_phosphate_diaphorase__NADPH_d__ L2 - http://ibj.pasteur.ac.ir/browse.php?a_id=450&sid=1&slc_lang=en DB - PRIME DP - Unbound Medicine ER -