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Comparison of enzyme immunoassay and immunodiffusion for the detection of canine blastomycosis.
Mycoses. 1990 Jul-Aug; 33(7-8):375-81.M

Abstract

Two Blastomyces dermatitidis commercial immunodiffusion antigens, Meridian Diagnostics and Nolan-Scott Laboratories, and two B. dermatitidis yeast phase lysate antigens, T-58 and K-Le, prepared in our laboratory were utilized in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in 61 serum specimens from dogs with blastomycosis. All antigens used in the ELISA were diluted to 50 ng of protein ml-1. Immunodiffusion (ID) tests were also performed on the sera using a Nolan-Scott ID antigen. Greater reactivity, based on the ELISA index value, was evidenced with the two yeast phase lysate antigens over the two commercial reagents in the ELISA. In addition the sensitivity of the ELISA with the T-58, K-Le and Meridian antigens was considerably greater than that of the ID test. The Nolan-Scott antigen, however, was able to detect more positive specimens when used in the ID procedure than in the ELISA. Therefore encouraging results were obtained with respect to the continued development and optimization of the ELISA using B. dermatitidis cell lysate antigens for the diagnosis of blastomycosis.

Authors+Show Affiliations

Seawell BW
Department of Biological Sciences, Idaho State University, Pocatello.
Scalarone GM
No affiliation info available

MeSH

AnimalsAntibodies, FungalAntigens, FungalBlastomycesBlastomycosisDog DiseasesDogsEnzyme-Linked Immunosorbent AssayImmunodiffusion

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2128645

Citation

Seawell, B W., and G M. Scalarone. "Comparison of Enzyme Immunoassay and Immunodiffusion for the Detection of Canine Blastomycosis." Mycoses, vol. 33, no. 7-8, 1990, pp. 375-81.
Seawell BW, Scalarone GM. Comparison of enzyme immunoassay and immunodiffusion for the detection of canine blastomycosis. Mycoses. 1990;33(7-8):375-81.
Seawell, B. W., & Scalarone, G. M. (1990). Comparison of enzyme immunoassay and immunodiffusion for the detection of canine blastomycosis. Mycoses, 33(7-8), 375-81.
Seawell BW, Scalarone GM. Comparison of Enzyme Immunoassay and Immunodiffusion for the Detection of Canine Blastomycosis. Mycoses. 1990 Jul-Aug;33(7-8):375-81. PubMed PMID: 2128645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of enzyme immunoassay and immunodiffusion for the detection of canine blastomycosis. AU - Seawell,B W, AU - Scalarone,G M, PY - 1990/7/1/pubmed PY - 1990/7/1/medline PY - 1990/7/1/entrez SP - 375 EP - 81 JF - Mycoses JO - Mycoses VL - 33 IS - 7-8 N2 - Two Blastomyces dermatitidis commercial immunodiffusion antigens, Meridian Diagnostics and Nolan-Scott Laboratories, and two B. dermatitidis yeast phase lysate antigens, T-58 and K-Le, prepared in our laboratory were utilized in an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in 61 serum specimens from dogs with blastomycosis. All antigens used in the ELISA were diluted to 50 ng of protein ml-1. Immunodiffusion (ID) tests were also performed on the sera using a Nolan-Scott ID antigen. Greater reactivity, based on the ELISA index value, was evidenced with the two yeast phase lysate antigens over the two commercial reagents in the ELISA. In addition the sensitivity of the ELISA with the T-58, K-Le and Meridian antigens was considerably greater than that of the ID test. The Nolan-Scott antigen, however, was able to detect more positive specimens when used in the ID procedure than in the ELISA. Therefore encouraging results were obtained with respect to the continued development and optimization of the ELISA using B. dermatitidis cell lysate antigens for the diagnosis of blastomycosis. SN - 0933-7407 UR - https://www.unboundmedicine.com/medline/citation/2128645/Comparison_of_enzyme_immunoassay_and_immunodiffusion_for_the_detection_of_canine_blastomycosis_ DB - PRIME DP - Unbound Medicine ER -