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Analysing complete genome sequence of swine hepatitis E virus (HEV), strain CHN-XJ-SW13 isolated from Xinjiang, China: putative host range, and disease severity determinants in HEV.
Infect Genet Evol. 2011 Apr; 11(3):618-23.IG

Abstract

Hepatitis E is a worldwide public health problem, particular in areas where hygiene conditions are poor. Hepatitis E virus (HEV) has at least four genotypes: genotypes 1 and 2 exclusively infect human; while genotypes 3 and 4, are considered to be a zoonotic agent, infecting both humans and animals. This study was aimed at determining why genotype 3 and 4 HEV strains isolated from swine are able to cross species borders, whereas genotype1 and 2 strains isolated from humans are not. The full length genome of the swine HEV isolate CHN-XJ-SW13 was amplified as overlapping fragments using reverse-transcription-nested polymerase chain reaction (RT-nPCR) and rapid amplification of cDNA ends (RACE). The sequence of CHN-XJ-SW13 was compared with those of 90 HEV strains covering genotype 1-4 retrieved from GenBank. Possible regions of the viral genome, specifying the host range of HEV or associated with the severity of hepatitis E disease, were then screened for with the aid of the ALIGNX sequences alignment software package. The CHN-XJ-SW13 swine HEV isolate was determined to be a novel subtype of genotype 4, whose sequence provided several valuable clues for tracing the sources of human HEV infection. 25 specific nucleotide positions were identified to possibly being involved specifying the host range of HEV or determining the severity of hepatitis E disease.

Authors+Show Affiliations

Department of Clinical Laboratory Medicine, Tianjin Medical University General Hospital, Tianjin, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21292034

Citation

Fu, Hongwei, et al. "Analysing Complete Genome Sequence of Swine Hepatitis E Virus (HEV), Strain CHN-XJ-SW13 Isolated From Xinjiang, China: Putative Host Range, and Disease Severity Determinants in HEV." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 11, no. 3, 2011, pp. 618-23.
Fu H, Wang L, Zhu Y, et al. Analysing complete genome sequence of swine hepatitis E virus (HEV), strain CHN-XJ-SW13 isolated from Xinjiang, China: putative host range, and disease severity determinants in HEV. Infect Genet Evol. 2011;11(3):618-23.
Fu, H., Wang, L., Zhu, Y., Geng, J., Li, L., Wang, X., Bu, Q., & Zhuang, H. (2011). Analysing complete genome sequence of swine hepatitis E virus (HEV), strain CHN-XJ-SW13 isolated from Xinjiang, China: putative host range, and disease severity determinants in HEV. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 11(3), 618-23. https://doi.org/10.1016/j.meegid.2011.01.018
Fu H, et al. Analysing Complete Genome Sequence of Swine Hepatitis E Virus (HEV), Strain CHN-XJ-SW13 Isolated From Xinjiang, China: Putative Host Range, and Disease Severity Determinants in HEV. Infect Genet Evol. 2011;11(3):618-23. PubMed PMID: 21292034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysing complete genome sequence of swine hepatitis E virus (HEV), strain CHN-XJ-SW13 isolated from Xinjiang, China: putative host range, and disease severity determinants in HEV. AU - Fu,Hongwei, AU - Wang,Ling, AU - Zhu,Yonghong, AU - Geng,Jiabao, AU - Li,Lingjun, AU - Wang,Xiaojuan, AU - Bu,Qiuning, AU - Zhuang,Hui, Y1 - 2011/02/01/ PY - 2010/11/24/received PY - 2011/01/12/revised PY - 2011/01/22/accepted PY - 2011/2/5/entrez PY - 2011/2/5/pubmed PY - 2011/10/1/medline SP - 618 EP - 23 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 11 IS - 3 N2 - Hepatitis E is a worldwide public health problem, particular in areas where hygiene conditions are poor. Hepatitis E virus (HEV) has at least four genotypes: genotypes 1 and 2 exclusively infect human; while genotypes 3 and 4, are considered to be a zoonotic agent, infecting both humans and animals. This study was aimed at determining why genotype 3 and 4 HEV strains isolated from swine are able to cross species borders, whereas genotype1 and 2 strains isolated from humans are not. The full length genome of the swine HEV isolate CHN-XJ-SW13 was amplified as overlapping fragments using reverse-transcription-nested polymerase chain reaction (RT-nPCR) and rapid amplification of cDNA ends (RACE). The sequence of CHN-XJ-SW13 was compared with those of 90 HEV strains covering genotype 1-4 retrieved from GenBank. Possible regions of the viral genome, specifying the host range of HEV or associated with the severity of hepatitis E disease, were then screened for with the aid of the ALIGNX sequences alignment software package. The CHN-XJ-SW13 swine HEV isolate was determined to be a novel subtype of genotype 4, whose sequence provided several valuable clues for tracing the sources of human HEV infection. 25 specific nucleotide positions were identified to possibly being involved specifying the host range of HEV or determining the severity of hepatitis E disease. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/21292034/Analysing_complete_genome_sequence_of_swine_hepatitis_E_virus__HEV__strain_CHN_XJ_SW13_isolated_from_Xinjiang_China:_putative_host_range_and_disease_severity_determinants_in_HEV_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(11)00021-9 DB - PRIME DP - Unbound Medicine ER -