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Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: acute toxicity, oxidative stress and oxidative damage.
Sci Total Environ. 2011 Mar 15; 409(8):1444-52.ST

Abstract

The acute toxicity and oxidative effects of nano-scale titanium dioxide, zinc oxide and their bulk counterparts in zebrafish were studied. It was found that although the size distribution of nanoparticles (NPs) was similar to that of the bulk particles in suspension, the acute toxicity of the TiO(2) NPs (96-h LC(50) of 124.5mg/L) to zebrafish was greater than that of the bulk TiO(2), which was essentially non-toxic. The acute toxicities observed for ZnO NPs, a bulk ZnO suspension, and a Zn(2+) solution were quite similar to each other (96-h LC(50) of 4.92, 3.31 and 8.06 mg/L, respectively). In order to explore the underlying toxicity mechanisms of NPs, ·OH radicals generated by NPs in suspensions and five biomarkers of oxidative effects, i.e. superoxide dismutase, catalase activities, malondialdehyde, reduced glutathione and protein carbonyl were investigated. Results showed that after the illumination for 96 h, the quantities of ·OH in the NP suspensions were much higher than ones in the bulk particles suspensions. The malondialdehyde content of zebrafish gills exposed to either illumination or dark were 217.2% and 174.3% of controls, respectively. This discrepancy indicates the occurrence of lipid peroxidation which is partly due to the generation of ·OH. In contrast, exposure to 5mg/L ZnO NPs and bulk ZnO suspension induced oxidative stress in the gills without oxidative damage. Oxidative effects were more severe in the livers, where the protein carbonyl content, in the light and dark groups exposed to 50mg/L TiO(2) NPs, was 178.1% and 139.7% of controls, respectively. The malondialdehyde levels in the liver of fish exposed to 5mg/L ZnO NPs and bulk ZnO were elevated (204.2% and 286.9% of controls, respectively). Additionally, gut tissues exhibited oxidative effects after exposure to NP suspensions. These results highlight the importance of a systematic assessment of metal oxide NP toxicity mechanisms.

Authors+Show Affiliations

Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21296382

Citation

Xiong, Daowen, et al. "Effects of Nano-scale TiO2, ZnO and Their Bulk Counterparts On Zebrafish: Acute Toxicity, Oxidative Stress and Oxidative Damage." The Science of the Total Environment, vol. 409, no. 8, 2011, pp. 1444-52.
Xiong D, Fang T, Yu L, et al. Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: acute toxicity, oxidative stress and oxidative damage. Sci Total Environ. 2011;409(8):1444-52.
Xiong, D., Fang, T., Yu, L., Sima, X., & Zhu, W. (2011). Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: acute toxicity, oxidative stress and oxidative damage. The Science of the Total Environment, 409(8), 1444-52. https://doi.org/10.1016/j.scitotenv.2011.01.015
Xiong D, et al. Effects of Nano-scale TiO2, ZnO and Their Bulk Counterparts On Zebrafish: Acute Toxicity, Oxidative Stress and Oxidative Damage. Sci Total Environ. 2011 Mar 15;409(8):1444-52. PubMed PMID: 21296382.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of nano-scale TiO2, ZnO and their bulk counterparts on zebrafish: acute toxicity, oxidative stress and oxidative damage. AU - Xiong,Daowen, AU - Fang,Tao, AU - Yu,Linpeng, AU - Sima,Xiaofeng, AU - Zhu,Wentao, PY - 2010/04/16/received PY - 2010/11/10/revised PY - 2011/01/07/accepted PY - 2011/2/8/entrez PY - 2011/2/8/pubmed PY - 2011/5/12/medline SP - 1444 EP - 52 JF - The Science of the total environment JO - Sci Total Environ VL - 409 IS - 8 N2 - The acute toxicity and oxidative effects of nano-scale titanium dioxide, zinc oxide and their bulk counterparts in zebrafish were studied. It was found that although the size distribution of nanoparticles (NPs) was similar to that of the bulk particles in suspension, the acute toxicity of the TiO(2) NPs (96-h LC(50) of 124.5mg/L) to zebrafish was greater than that of the bulk TiO(2), which was essentially non-toxic. The acute toxicities observed for ZnO NPs, a bulk ZnO suspension, and a Zn(2+) solution were quite similar to each other (96-h LC(50) of 4.92, 3.31 and 8.06 mg/L, respectively). In order to explore the underlying toxicity mechanisms of NPs, ·OH radicals generated by NPs in suspensions and five biomarkers of oxidative effects, i.e. superoxide dismutase, catalase activities, malondialdehyde, reduced glutathione and protein carbonyl were investigated. Results showed that after the illumination for 96 h, the quantities of ·OH in the NP suspensions were much higher than ones in the bulk particles suspensions. The malondialdehyde content of zebrafish gills exposed to either illumination or dark were 217.2% and 174.3% of controls, respectively. This discrepancy indicates the occurrence of lipid peroxidation which is partly due to the generation of ·OH. In contrast, exposure to 5mg/L ZnO NPs and bulk ZnO suspension induced oxidative stress in the gills without oxidative damage. Oxidative effects were more severe in the livers, where the protein carbonyl content, in the light and dark groups exposed to 50mg/L TiO(2) NPs, was 178.1% and 139.7% of controls, respectively. The malondialdehyde levels in the liver of fish exposed to 5mg/L ZnO NPs and bulk ZnO were elevated (204.2% and 286.9% of controls, respectively). Additionally, gut tissues exhibited oxidative effects after exposure to NP suspensions. These results highlight the importance of a systematic assessment of metal oxide NP toxicity mechanisms. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/21296382/Effects_of_nano_scale_TiO2_ZnO_and_their_bulk_counterparts_on_zebrafish:_acute_toxicity_oxidative_stress_and_oxidative_damage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(11)00052-0 DB - PRIME DP - Unbound Medicine ER -