Tags

Type your tag names separated by a space and hit enter

Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film.
Biosens Bioelectron. 2011 Mar 15; 26(7):3339-45.BB

Abstract

A rapid, convenient and accurate method for the simultaneous detection of guanine (G), adenine (A), thymine (T) and cytosine (C) was developed at a multiwalled carbon nanotube (MWCNT)/choline (Ch) monolayer-modified glassy carbon electrode (GCE). X-ray photoelectron spectroscopy data demonstrated that Ch was covalently immobilised on the surface of GCE through oxygen atom. The Ch monolayer provides a positively charged surface with -N(+)(CH(3))(3) polar groups, so that it can attract negatively charged MWCNTs to the surface. Consequently, the MWCNT/Ch film exhibited remarkable electrocatalytic activities towards the oxidation of G, A, T and C due to the advantages of high electrode activity, large surface area, prominent antifouling property, and high electron transfer kinetics. All purine and pyrimidine bases showed well-defined catalytic oxidation peaks at MWCNT/Ch/GCE. The peak separations between G and A, A and T, and T and C are 270, 200, and 190 mV, respectively, which are sufficiently large for their potential recognition and simultaneous detection in mixture. Under the optimum conditions, the designed MWCNT/Ch/GCE exhibited low detection limit, high sensitivity and wide linear range for simultaneous detection of G, A, T and C. Moreover, the proposed method was successfully applied to the assessment of G, A, T and C contents in a herring sperm DNA sample with satisfactory results.

Links

Publisher Full Text

Authors+Show Affiliations

Wang P
School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, PR China. wangpo@mail.ahnu.edu.cn
Wu H
No affiliation info available
Dai Z
No affiliation info available
Zou X
No affiliation info available

MeSH

AdenineAnimalsCholineCytosineDNAElectrochemical TechniquesFishesGuanineNanotubes, CarbonOxidation-ReductionReproducibility of ResultsSensitivity and SpecificityThymine

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21296567

Citation

Wang, Po, et al. "Simultaneous Detection of Guanine, Adenine, Thymine and Cytosine at Choline Monolayer Supported Multiwalled Carbon Nanotubes Film." Biosensors & Bioelectronics, vol. 26, no. 7, 2011, pp. 3339-45.
Wang P, Wu H, Dai Z, et al. Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film. Biosens Bioelectron. 2011;26(7):3339-45.
Wang, P., Wu, H., Dai, Z., & Zou, X. (2011). Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film. Biosensors & Bioelectronics, 26(7), 3339-45. https://doi.org/10.1016/j.bios.2011.01.011
Wang P, et al. Simultaneous Detection of Guanine, Adenine, Thymine and Cytosine at Choline Monolayer Supported Multiwalled Carbon Nanotubes Film. Biosens Bioelectron. 2011 Mar 15;26(7):3339-45. PubMed PMID: 21296567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous detection of guanine, adenine, thymine and cytosine at choline monolayer supported multiwalled carbon nanotubes film. AU - Wang,Po, AU - Wu,Hai, AU - Dai,Zong, AU - Zou,Xiaoyong, Y1 - 2011/01/19/ PY - 2010/10/14/received PY - 2011/01/06/revised PY - 2011/01/07/accepted PY - 2011/2/8/entrez PY - 2011/2/8/pubmed PY - 2011/6/7/medline SP - 3339 EP - 45 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 7 N2 - A rapid, convenient and accurate method for the simultaneous detection of guanine (G), adenine (A), thymine (T) and cytosine (C) was developed at a multiwalled carbon nanotube (MWCNT)/choline (Ch) monolayer-modified glassy carbon electrode (GCE). X-ray photoelectron spectroscopy data demonstrated that Ch was covalently immobilised on the surface of GCE through oxygen atom. The Ch monolayer provides a positively charged surface with -N(+)(CH(3))(3) polar groups, so that it can attract negatively charged MWCNTs to the surface. Consequently, the MWCNT/Ch film exhibited remarkable electrocatalytic activities towards the oxidation of G, A, T and C due to the advantages of high electrode activity, large surface area, prominent antifouling property, and high electron transfer kinetics. All purine and pyrimidine bases showed well-defined catalytic oxidation peaks at MWCNT/Ch/GCE. The peak separations between G and A, A and T, and T and C are 270, 200, and 190 mV, respectively, which are sufficiently large for their potential recognition and simultaneous detection in mixture. Under the optimum conditions, the designed MWCNT/Ch/GCE exhibited low detection limit, high sensitivity and wide linear range for simultaneous detection of G, A, T and C. Moreover, the proposed method was successfully applied to the assessment of G, A, T and C contents in a herring sperm DNA sample with satisfactory results. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21296567/Simultaneous_detection_of_guanine_adenine_thymine_and_cytosine_at_choline_monolayer_supported_multiwalled_carbon_nanotubes_film_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00035-2 DB - PRIME DP - Unbound Medicine ER -