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Relationship between thyroid autoantibody spectrotype and IgG subclass.
Autoimmunity. 1990; 6(1-2):37-45.A

Abstract

The relationship between spectrotype and IgG subclass of autoantibodies to thyroglobulin (Tg) and thyroid peroxidase (TPO) has been investigated using sera from Hashimoto and Graves' patients. Isoelectric focussing (IEF) was carried out in gels over the range pI 3.5-9.5 followed by transblotting to nitrocellulose and probing the filters using 125I-labelled TPO and Tg. As has been shown previously for Tg antibodies, TPO antibody focussed over different pI values in different patients but the spectrotypes for individuals were constant over 2-5 years. Further, the pI values for TPO and Tg autoantibodies appeared to be related to IgG subclass, for example IgG1 Tg antibodies tended to focus nearer the cathode (pI 8.0-9.5) than IgG4 antibodies (pI 6.5-8.5) while antibodies of subclasses IgG1 + 2 + 4 produced spectrotypes covering a broad pI range (5.7-9.5). Consequently, it seems likely that the characteristic spectrotypes described by others for Tg autoantibodies, and those we now report for TPO antibodies, reflect the IgG subclass "fingerprints" which we suggest may be a measure of the ability of an individual to respond to different epitopes on these two thyroid antigens.

Authors+Show Affiliations

Department of Medicine, University of Wales College of Medicine, Cardiff, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2129769

Citation

Fukuma, N, et al. "Relationship Between Thyroid Autoantibody Spectrotype and IgG Subclass." Autoimmunity, vol. 6, no. 1-2, 1990, pp. 37-45.
Fukuma N, McLachlan SM, Petersen VB, et al. Relationship between thyroid autoantibody spectrotype and IgG subclass. Autoimmunity. 1990;6(1-2):37-45.
Fukuma, N., McLachlan, S. M., Petersen, V. B., Beever, K., & Smith, B. R. (1990). Relationship between thyroid autoantibody spectrotype and IgG subclass. Autoimmunity, 6(1-2), 37-45.
Fukuma N, et al. Relationship Between Thyroid Autoantibody Spectrotype and IgG Subclass. Autoimmunity. 1990;6(1-2):37-45. PubMed PMID: 2129769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between thyroid autoantibody spectrotype and IgG subclass. AU - Fukuma,N, AU - McLachlan,S M, AU - Petersen,V B, AU - Beever,K, AU - Smith,B R, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 37 EP - 45 JF - Autoimmunity JO - Autoimmunity VL - 6 IS - 1-2 N2 - The relationship between spectrotype and IgG subclass of autoantibodies to thyroglobulin (Tg) and thyroid peroxidase (TPO) has been investigated using sera from Hashimoto and Graves' patients. Isoelectric focussing (IEF) was carried out in gels over the range pI 3.5-9.5 followed by transblotting to nitrocellulose and probing the filters using 125I-labelled TPO and Tg. As has been shown previously for Tg antibodies, TPO antibody focussed over different pI values in different patients but the spectrotypes for individuals were constant over 2-5 years. Further, the pI values for TPO and Tg autoantibodies appeared to be related to IgG subclass, for example IgG1 Tg antibodies tended to focus nearer the cathode (pI 8.0-9.5) than IgG4 antibodies (pI 6.5-8.5) while antibodies of subclasses IgG1 + 2 + 4 produced spectrotypes covering a broad pI range (5.7-9.5). Consequently, it seems likely that the characteristic spectrotypes described by others for Tg autoantibodies, and those we now report for TPO antibodies, reflect the IgG subclass "fingerprints" which we suggest may be a measure of the ability of an individual to respond to different epitopes on these two thyroid antigens. SN - 0891-6934 UR - https://www.unboundmedicine.com/medline/citation/2129769/Relationship_between_thyroid_autoantibody_spectrotype_and_IgG_subclass_ L2 - http://www.tandfonline.com/doi/full/10.3109/08916939008993368 DB - PRIME DP - Unbound Medicine ER -