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Polymeric IgA antibody to gliadin in the serum of patients with coeliac disease.
J Gastroenterol Hepatol. 1990 Nov-Dec; 5(6):675-81.JG

Abstract

Secretory component (SC) binding assays which detect polymeric IgA (pIgA) in serum were used to measure serum antigliadin pIgA and total pIgA in patients with coeliac disease. Total IgA antigliadin antibody in serum and intestinal fluid was measured by enzyme linked immunosorbent assay (ELISA). The relationship of pIgA antibody to dietary gluten and the antigliadin IgA antibody in intestinal fluid was examined. Twenty-nine serum samples were assayed, twelve from patients ingesting gluten and seventeen from patients who had excluded gluten from their diet for 6 months. Eight of these were paired samples from 4 adults on and off gluten. In addition, paired samples of both intestinal fluid and serum were obtained from 7 children on and off gluten. Polymeric IgA antibody to gliadin was detected in 11 of 12 subjects on gluten but in only 3 of 17 who had excluded gluten. Three of the four adults from whom paired serum samples were obtained had pIgA antigliadin, but only while on gluten. Three of the seven children in whom intestinal and serum antibody were assayed had pIgA to gliadin, which could not be detected after exclusion of gluten, although their intestinal antibody level remained elevated. There was no change in total pIgA levels with diet although the levels were higher than those seen in normal subjects. We conclude that pIgA antibody to gliadin is frequently found in the serum of coeliac patients ingesting gluten. It disappears with gluten elimination at a time when the IgA antigliadin antibody in intestinal fluid has not altered.

Authors+Show Affiliations

Department of Medicine, Royal Adelaide Hospital, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2129839

Citation

Bartholomeusz, R C., et al. "Polymeric IgA Antibody to Gliadin in the Serum of Patients With Coeliac Disease." Journal of Gastroenterology and Hepatology, vol. 5, no. 6, 1990, pp. 675-81.
Bartholomeusz RC, Labrooy JT, Davidson GP, et al. Polymeric IgA antibody to gliadin in the serum of patients with coeliac disease. J Gastroenterol Hepatol. 1990;5(6):675-81.
Bartholomeusz, R. C., Labrooy, J. T., Davidson, G. P., Hetzel, P., Johnson, R. B., & Shearman, D. J. (1990). Polymeric IgA antibody to gliadin in the serum of patients with coeliac disease. Journal of Gastroenterology and Hepatology, 5(6), 675-81.
Bartholomeusz RC, et al. Polymeric IgA Antibody to Gliadin in the Serum of Patients With Coeliac Disease. J Gastroenterol Hepatol. 1990 Nov-Dec;5(6):675-81. PubMed PMID: 2129839.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polymeric IgA antibody to gliadin in the serum of patients with coeliac disease. AU - Bartholomeusz,R C, AU - Labrooy,J T, AU - Davidson,G P, AU - Hetzel,P, AU - Johnson,R B, AU - Shearman,D J, PY - 1990/11/1/pubmed PY - 1990/11/1/medline PY - 1990/11/1/entrez SP - 675 EP - 81 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 5 IS - 6 N2 - Secretory component (SC) binding assays which detect polymeric IgA (pIgA) in serum were used to measure serum antigliadin pIgA and total pIgA in patients with coeliac disease. Total IgA antigliadin antibody in serum and intestinal fluid was measured by enzyme linked immunosorbent assay (ELISA). The relationship of pIgA antibody to dietary gluten and the antigliadin IgA antibody in intestinal fluid was examined. Twenty-nine serum samples were assayed, twelve from patients ingesting gluten and seventeen from patients who had excluded gluten from their diet for 6 months. Eight of these were paired samples from 4 adults on and off gluten. In addition, paired samples of both intestinal fluid and serum were obtained from 7 children on and off gluten. Polymeric IgA antibody to gliadin was detected in 11 of 12 subjects on gluten but in only 3 of 17 who had excluded gluten. Three of the four adults from whom paired serum samples were obtained had pIgA antigliadin, but only while on gluten. Three of the seven children in whom intestinal and serum antibody were assayed had pIgA to gliadin, which could not be detected after exclusion of gluten, although their intestinal antibody level remained elevated. There was no change in total pIgA levels with diet although the levels were higher than those seen in normal subjects. We conclude that pIgA antibody to gliadin is frequently found in the serum of coeliac patients ingesting gluten. It disappears with gluten elimination at a time when the IgA antigliadin antibody in intestinal fluid has not altered. SN - 0815-9319 UR - https://www.unboundmedicine.com/medline/citation/2129839/Polymeric_IgA_antibody_to_gliadin_in_the_serum_of_patients_with_coeliac_disease_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0815-9319&date=1990&volume=5&issue=6&spage=675 DB - PRIME DP - Unbound Medicine ER -