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[Structural alterations of the smooth muscle and of the EGFR expression and C-kit in congenital pyeloureteral stenosis. Relationship with its pathogenesis].
Cir Pediatr. 2010 Apr; 23(2):82-7.CP

Abstract

PURPOSE

To assess the role that the muscular dysplasia, epidermal growth factor (EGFR) and interstitial cells of Cajal (ICC) C-kit +, may have in the pathophysiology of pyeloureteral stenosis (EPU).

MATERIALS AND METHODS

30 samples were studied UPJ (ureteropelvic junction), with 25 UPJ obstruction and 5 controls. Performed with Masson's trichrome staining and immunohistochemical techniques for smooth muscle actin, EGFR (epidermal growth factor) and CD117 (C-kit) (Dako). We compared the results statistically.

RESULTS

The control group was diffuse positivity of EGFR in the muscle layer in samples of EPU was positive in 4%, weak in 16% and negative in 80%. The controls are C-kit + ICC between the muscle cells showed a decreased density in the samples of obstruction. Masson's trichrome highlighted the increase of inter-and intramuscular collagen and attenuated muscle fibers, thinner and individualized, in the inner muscular layer of the cases of EPU also evident with the actin. Differences between groups were statistically significant (p < 0.001).

CONCLUSIONS

Our results suggest that dysplastic changes in the muscle layer, the low expression of EGFR and the decrease or absence of C-kit + ICC, may participate in the pathophysiology of UPJ obstruction.

Authors+Show Affiliations

Servicio de Cirugía Pediátrica, Hospital Materno-Infantil, Hospital Universitario Reina Sofía, Córdoba.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

spa

PubMed ID

21298915

Citation

Vargas Cruz, V, et al. "[Structural Alterations of the Smooth Muscle and of the EGFR Expression and C-kit in Congenital Pyeloureteral Stenosis. Relationship With Its Pathogenesis]." Cirugia Pediatrica : Organo Oficial De La Sociedad Espanola De Cirugia Pediatrica, vol. 23, no. 2, 2010, pp. 82-7.
Vargas Cruz V, García Ceballos A, Ruiz Hierro C, et al. [Structural alterations of the smooth muscle and of the EGFR expression and C-kit in congenital pyeloureteral stenosis. Relationship with its pathogenesis]. Cir Pediatr. 2010;23(2):82-7.
Vargas Cruz, V., García Ceballos, A., Ruiz Hierro, C., Moreno Rodríguez, M. M., Escassi Gil, A., Garrido Pérez, J. I., & Paredes Esteban, R. M. (2010). [Structural alterations of the smooth muscle and of the EGFR expression and C-kit in congenital pyeloureteral stenosis. Relationship with its pathogenesis]. Cirugia Pediatrica : Organo Oficial De La Sociedad Espanola De Cirugia Pediatrica, 23(2), 82-7.
Vargas Cruz V, et al. [Structural Alterations of the Smooth Muscle and of the EGFR Expression and C-kit in Congenital Pyeloureteral Stenosis. Relationship With Its Pathogenesis]. Cir Pediatr. 2010;23(2):82-7. PubMed PMID: 21298915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Structural alterations of the smooth muscle and of the EGFR expression and C-kit in congenital pyeloureteral stenosis. Relationship with its pathogenesis]. AU - Vargas Cruz,V, AU - García Ceballos,A, AU - Ruiz Hierro,C, AU - Moreno Rodríguez,M M, AU - Escassi Gil,A, AU - Garrido Pérez,J I, AU - Paredes Esteban,R M, PY - 2011/2/9/entrez PY - 2011/2/9/pubmed PY - 2011/2/25/medline SP - 82 EP - 7 JF - Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica JO - Cir Pediatr VL - 23 IS - 2 N2 - PURPOSE: To assess the role that the muscular dysplasia, epidermal growth factor (EGFR) and interstitial cells of Cajal (ICC) C-kit +, may have in the pathophysiology of pyeloureteral stenosis (EPU). MATERIALS AND METHODS: 30 samples were studied UPJ (ureteropelvic junction), with 25 UPJ obstruction and 5 controls. Performed with Masson's trichrome staining and immunohistochemical techniques for smooth muscle actin, EGFR (epidermal growth factor) and CD117 (C-kit) (Dako). We compared the results statistically. RESULTS: The control group was diffuse positivity of EGFR in the muscle layer in samples of EPU was positive in 4%, weak in 16% and negative in 80%. The controls are C-kit + ICC between the muscle cells showed a decreased density in the samples of obstruction. Masson's trichrome highlighted the increase of inter-and intramuscular collagen and attenuated muscle fibers, thinner and individualized, in the inner muscular layer of the cases of EPU also evident with the actin. Differences between groups were statistically significant (p < 0.001). CONCLUSIONS: Our results suggest that dysplastic changes in the muscle layer, the low expression of EGFR and the decrease or absence of C-kit + ICC, may participate in the pathophysiology of UPJ obstruction. SN - 0214-1221 UR - https://www.unboundmedicine.com/medline/citation/21298915/[Structural_alterations_of_the_smooth_muscle_and_of_the_EGFR_expression_and_C_kit_in_congenital_pyeloureteral_stenosis__Relationship_with_its_pathogenesis]_ L2 - https://antibodies.cancer.gov/detail/CPTC-EGFR-1 DB - PRIME DP - Unbound Medicine ER -