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Changes of platelet antioxidative enzymes during oxidative stress: the protective effect of polyphenol-rich extract from berries of Aronia melanocarpa and grape seeds.
Platelets. 2011; 22(5):385-9.P

Abstract

Aronia melanocarpa fruits (Rosaceae) and grape seeds (seeds of Vitis vinifera, Vitaceae) are two of the richest plant sources of phenolic substances, and they have been shown to have various biological activities. The aim of the present study was to investigate and compare the action of phenolic extracts (at concentrations 5-100 µg/mL) of two different plants, berries of A. melanocarpa (chokebbery) and grape seeds, on the activities of various antioxidative enzymes, the amount of glutathione (as an important component of redox status) in control the platelets and platelets treated with H(2)O(2) (the strong physiological oxidant) in vitro. The properties of these two tested extracts were also compared with the action of a well characterized antioxidative and antiplatelet commercial monomeric polyphenol - resveratrol. The extract from berries of A. melanocarpa, like the extract from grape seeds, reduced the changes in activities of different antioxidative enzymes (glutathione peroxidase, superoxide dismutase, and catalase) in platelets treated with H(2)O(2). The action of the two tested plant extracts and H(2)O(2) evoked a significant increase of reduced glutathione in platelets compared with platelets treated with H(2)O(2) only. Comparative studies indicate that the two tested plant extracts had similar antioxidative properties, and were found to be more reactive in blood platelets than the solution of resveratrol.

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Authors+Show Affiliations

Kedzierska M
Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Poland.
Olas B
No affiliation info available
Wachowicz B
No affiliation info available
Stochmal A
No affiliation info available
Oleszek W
No affiliation info available
Erler J
No affiliation info available

MeSH

AdultAntioxidantsBlood PlateletsCatalaseCells, CulturedChromatography, High Pressure LiquidFlavonoidsFruitGlutathioneGlutathione PeroxidaseHumansHydrogen PeroxideMaleOxidative StressPhenolsPhotiniaPlant ExtractsResveratrolSeedsStilbenesSuperoxide DismutaseVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21299394

Citation

Kedzierska, Magdalena, et al. "Changes of Platelet Antioxidative Enzymes During Oxidative Stress: the Protective Effect of Polyphenol-rich Extract From Berries of Aronia Melanocarpa and Grape Seeds." Platelets, vol. 22, no. 5, 2011, pp. 385-9.
Kedzierska M, Olas B, Wachowicz B, et al. Changes of platelet antioxidative enzymes during oxidative stress: the protective effect of polyphenol-rich extract from berries of Aronia melanocarpa and grape seeds. Platelets. 2011;22(5):385-9.
Kedzierska, M., Olas, B., Wachowicz, B., Stochmal, A., Oleszek, W., & Erler, J. (2011). Changes of platelet antioxidative enzymes during oxidative stress: the protective effect of polyphenol-rich extract from berries of Aronia melanocarpa and grape seeds. Platelets, 22(5), 385-9. https://doi.org/10.3109/09537104.2010.545151
Kedzierska M, et al. Changes of Platelet Antioxidative Enzymes During Oxidative Stress: the Protective Effect of Polyphenol-rich Extract From Berries of Aronia Melanocarpa and Grape Seeds. Platelets. 2011;22(5):385-9. PubMed PMID: 21299394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes of platelet antioxidative enzymes during oxidative stress: the protective effect of polyphenol-rich extract from berries of Aronia melanocarpa and grape seeds. AU - Kedzierska,Magdalena, AU - Olas,Beata, AU - Wachowicz,Barbara, AU - Stochmal,Anna, AU - Oleszek,Wiesław, AU - Erler,Joachim, Y1 - 2011/02/07/ PY - 2011/2/9/entrez PY - 2011/2/9/pubmed PY - 2011/12/13/medline SP - 385 EP - 9 JF - Platelets JO - Platelets VL - 22 IS - 5 N2 - Aronia melanocarpa fruits (Rosaceae) and grape seeds (seeds of Vitis vinifera, Vitaceae) are two of the richest plant sources of phenolic substances, and they have been shown to have various biological activities. The aim of the present study was to investigate and compare the action of phenolic extracts (at concentrations 5-100 µg/mL) of two different plants, berries of A. melanocarpa (chokebbery) and grape seeds, on the activities of various antioxidative enzymes, the amount of glutathione (as an important component of redox status) in control the platelets and platelets treated with H(2)O(2) (the strong physiological oxidant) in vitro. The properties of these two tested extracts were also compared with the action of a well characterized antioxidative and antiplatelet commercial monomeric polyphenol - resveratrol. The extract from berries of A. melanocarpa, like the extract from grape seeds, reduced the changes in activities of different antioxidative enzymes (glutathione peroxidase, superoxide dismutase, and catalase) in platelets treated with H(2)O(2). The action of the two tested plant extracts and H(2)O(2) evoked a significant increase of reduced glutathione in platelets compared with platelets treated with H(2)O(2) only. Comparative studies indicate that the two tested plant extracts had similar antioxidative properties, and were found to be more reactive in blood platelets than the solution of resveratrol. SN - 1369-1635 UR - https://www.unboundmedicine.com/medline/citation/21299394/Changes_of_platelet_antioxidative_enzymes_during_oxidative_stress:_the_protective_effect_of_polyphenol_rich_extract_from_berries_of_Aronia_melanocarpa_and_grape_seeds_ L2 - https://www.tandfonline.com/doi/full/10.3109/09537104.2010.545151 DB - PRIME DP - Unbound Medicine ER -