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Iron absorption in hepcidin1 knockout mice.
Br J Nutr. 2011 Jun; 105(11):1583-91.BJ

Abstract

Hepcidin, the Fe-regulatory peptide, has been shown to inhibit Fe absorption and reticuloendothelial Fe recycling. The present study was conducted to explore the mechanism of in vivo Fe regulation through genetic disruption of hepcidin1 and acute effects of hepcidin treatment in hepcidin1 knockout (Hepc1-/-) and heterozygous mice. Hepcidin1 disruption resulted in significantly increased intestinal Fe uptake. Hepcidin injection inhibited Fe absorption in both genotypes, but the effects were more evident in the knockout mice. Hepcidin administration was also associated with decreased membrane localisation of ferroportin in the duodenum, liver and, most significantly, in the spleen of Hepc1-/- mice. Hypoferraemia was induced in heterozygous mice by hepcidin treatment, but not in Hepc1-/- mice, 4 h after injection. Interestingly, Fe absorption and serum Fe levels in Hepc1-/- and heterozygous mice fed a low-Fe diet were not affected by hepcidin injection. The present study demonstrates that hepcidin deficiency causes increased Fe absorption. The effects of hepcidin were abolished by dietary Fe deficiency, indicating that the response to hepcidin may be influenced by dietary Fe level or Fe status.

Authors+Show Affiliations

Nutritional Sciences Research Division, King's College London, 150 Stamford Street, London SE1 9NH, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21303570

Citation

Masaratana, Patarabutr, et al. "Iron Absorption in Hepcidin1 Knockout Mice." The British Journal of Nutrition, vol. 105, no. 11, 2011, pp. 1583-91.
Masaratana P, Laftah AH, Latunde-Dada GO, et al. Iron absorption in hepcidin1 knockout mice. Br J Nutr. 2011;105(11):1583-91.
Masaratana, P., Laftah, A. H., Latunde-Dada, G. O., Vaulont, S., Simpson, R. J., & McKie, A. T. (2011). Iron absorption in hepcidin1 knockout mice. The British Journal of Nutrition, 105(11), 1583-91. https://doi.org/10.1017/S0007114510005507
Masaratana P, et al. Iron Absorption in Hepcidin1 Knockout Mice. Br J Nutr. 2011;105(11):1583-91. PubMed PMID: 21303570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron absorption in hepcidin1 knockout mice. AU - Masaratana,Patarabutr, AU - Laftah,Abas H, AU - Latunde-Dada,Gladys O, AU - Vaulont,Sophie, AU - Simpson,Robert J, AU - McKie,Andrew T, Y1 - 2011/02/08/ PY - 2011/2/10/entrez PY - 2011/2/10/pubmed PY - 2011/8/30/medline SP - 1583 EP - 91 JF - The British journal of nutrition JO - Br J Nutr VL - 105 IS - 11 N2 - Hepcidin, the Fe-regulatory peptide, has been shown to inhibit Fe absorption and reticuloendothelial Fe recycling. The present study was conducted to explore the mechanism of in vivo Fe regulation through genetic disruption of hepcidin1 and acute effects of hepcidin treatment in hepcidin1 knockout (Hepc1-/-) and heterozygous mice. Hepcidin1 disruption resulted in significantly increased intestinal Fe uptake. Hepcidin injection inhibited Fe absorption in both genotypes, but the effects were more evident in the knockout mice. Hepcidin administration was also associated with decreased membrane localisation of ferroportin in the duodenum, liver and, most significantly, in the spleen of Hepc1-/- mice. Hypoferraemia was induced in heterozygous mice by hepcidin treatment, but not in Hepc1-/- mice, 4 h after injection. Interestingly, Fe absorption and serum Fe levels in Hepc1-/- and heterozygous mice fed a low-Fe diet were not affected by hepcidin injection. The present study demonstrates that hepcidin deficiency causes increased Fe absorption. The effects of hepcidin were abolished by dietary Fe deficiency, indicating that the response to hepcidin may be influenced by dietary Fe level or Fe status. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/21303570/Iron_absorption_in_hepcidin1_knockout_mice_ L2 - https://www.cambridge.org/core/product/identifier/S0007114510005507/type/journal_article DB - PRIME DP - Unbound Medicine ER -