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HCV subtype characterization among injection drug users: implication for a crucial role of Zhenjiang in HCV transmission in China.
PLoS One. 2011 Feb 03; 6(2):e16817.Plos

Abstract

BACKGROUND

HCV transmission is closely associated with drug-trafficking routes in China. However, the transmission route of HCV in Eastern China remains unclear. Here, we investigate the role of Zhenjiang city of Jiangsu province, an important transportation hub linking Shanghai with other regions of China, in HCV transmission.

METHODOLOGY/PRINCIPAL FINDINGS

A total of 141 whole blood samples were collected from injection drug users (IDUs) in Zhenjiang and then tested for HCV infection. Of them, 115 HCV positive plasmas were subjected to RNA extraction, RT-PCR amplification, and sequencing. The subtype characterization and the evolutionary origin of HCV strains circulating in Zhenjiang were determined using polygenetic or phylogeographic analyses. Seven HCV subtypes 1b, 2a, 3a, 3b, 6a, 6e and 6n were detected among Zhenjiang IDUs, showing a complex HCV epidemic. The most predominant subtypes were 3a (38%) and 1b (26.8%). Among these subtypes, subtypes 3b, 6n and 6e originated from Southwestern China (i.e., Yunnan and/or Guangxi), subtypes 2a and 6a from Southern China (i.e., Guangdong), subtype 1b from Central (i.e., Henan) and Northwestern (i.e., Xinjiang) China, and subtype 3a from Southwestern (i.e., Yunnan) and Northwestern (i.e., Xinjiang) China. From Zhenjiang, subtypes 1b and 2a were further spread to Eastern (i.e., Shanghai) and Northern (i.e., Beijing) China, respectively.

CONCLUSIONS/SIGNIFICANCE

The mixing of seven HCV subtypes in Zhenjiang from all quarters of China indicates that as an important middle station, Zhenjiang plays a crucial role in HCV transmission, just as it is important in population migration between other regions of China and Eastern China.

Authors+Show Affiliations

Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China. zhangcy1999@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21304823

Citation

Zhang, Chiyu, et al. "HCV Subtype Characterization Among Injection Drug Users: Implication for a Crucial Role of Zhenjiang in HCV Transmission in China." PloS One, vol. 6, no. 2, 2011, pp. e16817.
Zhang C, Wu N, Liu J, et al. HCV subtype characterization among injection drug users: implication for a crucial role of Zhenjiang in HCV transmission in China. PLoS One. 2011;6(2):e16817.
Zhang, C., Wu, N., Liu, J., Ge, Q., Huang, Y., Ren, Q., Feng, Q., & He, G. (2011). HCV subtype characterization among injection drug users: implication for a crucial role of Zhenjiang in HCV transmission in China. PloS One, 6(2), e16817. https://doi.org/10.1371/journal.pone.0016817
Zhang C, et al. HCV Subtype Characterization Among Injection Drug Users: Implication for a Crucial Role of Zhenjiang in HCV Transmission in China. PLoS One. 2011 Feb 3;6(2):e16817. PubMed PMID: 21304823.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HCV subtype characterization among injection drug users: implication for a crucial role of Zhenjiang in HCV transmission in China. AU - Zhang,Chiyu, AU - Wu,Nana, AU - Liu,Jun, AU - Ge,Qinjuan, AU - Huang,Yan, AU - Ren,Qian, AU - Feng,Qingchuan, AU - He,Guangli, Y1 - 2011/02/03/ PY - 2010/09/30/received PY - 2011/01/11/accepted PY - 2011/2/10/entrez PY - 2011/2/10/pubmed PY - 2011/9/2/medline SP - e16817 EP - e16817 JF - PloS one JO - PLoS One VL - 6 IS - 2 N2 - BACKGROUND: HCV transmission is closely associated with drug-trafficking routes in China. However, the transmission route of HCV in Eastern China remains unclear. Here, we investigate the role of Zhenjiang city of Jiangsu province, an important transportation hub linking Shanghai with other regions of China, in HCV transmission. METHODOLOGY/PRINCIPAL FINDINGS: A total of 141 whole blood samples were collected from injection drug users (IDUs) in Zhenjiang and then tested for HCV infection. Of them, 115 HCV positive plasmas were subjected to RNA extraction, RT-PCR amplification, and sequencing. The subtype characterization and the evolutionary origin of HCV strains circulating in Zhenjiang were determined using polygenetic or phylogeographic analyses. Seven HCV subtypes 1b, 2a, 3a, 3b, 6a, 6e and 6n were detected among Zhenjiang IDUs, showing a complex HCV epidemic. The most predominant subtypes were 3a (38%) and 1b (26.8%). Among these subtypes, subtypes 3b, 6n and 6e originated from Southwestern China (i.e., Yunnan and/or Guangxi), subtypes 2a and 6a from Southern China (i.e., Guangdong), subtype 1b from Central (i.e., Henan) and Northwestern (i.e., Xinjiang) China, and subtype 3a from Southwestern (i.e., Yunnan) and Northwestern (i.e., Xinjiang) China. From Zhenjiang, subtypes 1b and 2a were further spread to Eastern (i.e., Shanghai) and Northern (i.e., Beijing) China, respectively. CONCLUSIONS/SIGNIFICANCE: The mixing of seven HCV subtypes in Zhenjiang from all quarters of China indicates that as an important middle station, Zhenjiang plays a crucial role in HCV transmission, just as it is important in population migration between other regions of China and Eastern China. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/21304823/HCV_subtype_characterization_among_injection_drug_users:_implication_for_a_crucial_role_of_Zhenjiang_in_HCV_transmission_in_China_ L2 - https://dx.plos.org/10.1371/journal.pone.0016817 DB - PRIME DP - Unbound Medicine ER -