TY - JOUR T1 - A liquid chromatography-mass spectrometry method for nicotine and cotinine; utility in screening tobacco exposure in patients taking amiodarone. AU - Gabr,Raniah Q, AU - Elsherbiny,Marwa E, AU - Somayaji,Vishwa, AU - Pollak,P Timothy, AU - Brocks,Dion R, Y1 - 2011/02/10/ PY - 2010/09/29/received PY - 2010/10/27/revised PY - 2010/10/29/accepted PY - 2011/2/11/entrez PY - 2011/2/11/pubmed PY - 2012/1/19/medline SP - 1124 EP - 31 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 25 IS - 10 N2 - A liquid chromatographic mass spectrometric (LC-MS) assay for the quantification of nicotine and cotinine in human specimens was developed. Human serum and urine (100 μL) were subjected to liquid-liquid extraction. For glucuronidated cotinine, serum was alkalinized and hydrolyzed before extraction. The dried samples were reconstituted and run using gradient flow reverse-phase liquid chromatography with MS detection. The ions utilized for quantification of nicotine, cotinine and milrinone (internal standard) were 162.8, 176.9 and 211.9 m/z, respectively. The mean recoveries were over 80% for cotinine and nicotine with excellent linearity between nominal concentrations and peak area ratios, over a wide concentration range. The percentage coefficient of variation and mean error of the inter- and intra-day validations were <15% for nicotine and cotinine. Analysis of serum from cardiac patients receiving amiodarone suggested that a number of patients were either active smokers or exposed to second-hand smoke. Significant concentrations of nicotine and cotinine were measured in the urine of a known smoking volunteer. The method was highly specific, sensitive and applicable as a tool in detecting and monitoring the passive exposure to tobacco smoke using small specimen volumes (0.1 mL). SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/21308701/A_liquid_chromatography_mass_spectrometry_method_for_nicotine_and_cotinine DB - PRIME DP - Unbound Medicine ER -