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Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen.

Abstract

BACKGROUND

Kiwifruit is an important cause of food allergy. A high amount of a protein with a molecular mass compatible with that of Bet v 1 was observed in the kiwifruit extract.

OBJECTIVE

To identify and characterize kirola, the 17-kDa protein of green kiwifruit (Act d 11).

METHODS

Act d 11 was purified from green kiwifruit. Its primary structure was obtained by direct protein sequencing. The IgE binding was investigated by skin testing, immunoblotting, inhibition tests, and detection by the ISAC microarray in an Italian cohort and in selected Bet v 1-sensitized Austrian patients. A clinical evaluation of kiwi allergy was carried out.

RESULTS

Act d 11 was identified as a member of the major latex protein/ripening-related protein (MLP/RRP) family. IgE binding to Act d 11 was shown by all the applied testing. Patients tested positive for Act d 11 and reporting symptoms on kiwifruit exposure were found within the Bet v 1-positive subset rather than within the population selected for highly reliable history of allergic reactions to kiwifruit. Epidemiology of Act d 11 IgE reactivity was documented in the two cohorts. IgE co-recognition of Act d 11 within the Bet v 1-like molecules is documented using the microarray IgE inhibition assay.

CONCLUSIONS

Act d 11 is the first member of the MLP/RRP protein family to be described as an allergen. It displays IgE co-recognition with allergens belonging to the PR-10 family, including Bet v 1.

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  • Authors+Show Affiliations

    ,

    Institute of Protein Biochemistry, CNR, Naples, Italy.

    , , , , , , , , ,

    Source

    Allergy 66:7 2011 Jul pg 870-7

    MeSH

    Actinidia
    Adolescent
    Adult
    Aged
    Allergens
    Amino Acid Sequence
    Antigens, Plant
    Austria
    Child
    Child, Preschool
    Female
    Food Hypersensitivity
    Fruit
    Humans
    Immunoglobulin E
    Italy
    Male
    Middle Aged
    Molecular Sequence Data
    Plant Proteins
    Sequence Alignment
    Skin Tests
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    21309790

    Citation

    D'Avino, R, et al. "Kiwifruit Act D 11 Is the First Member of the Ripening-related Protein Family Identified as an Allergen." Allergy, vol. 66, no. 7, 2011, pp. 870-7.
    D'Avino R, Bernardi ML, Wallner M, et al. Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen. Allergy. 2011;66(7):870-7.
    D'Avino, R., Bernardi, M. L., Wallner, M., Palazzo, P., Camardella, L., Tuppo, L., ... Mari, A. (2011). Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen. Allergy, 66(7), pp. 870-7. doi:10.1111/j.1398-9995.2011.02555.x.
    D'Avino R, et al. Kiwifruit Act D 11 Is the First Member of the Ripening-related Protein Family Identified as an Allergen. Allergy. 2011;66(7):870-7. PubMed PMID: 21309790.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen. AU - D'Avino,R, AU - Bernardi,M L, AU - Wallner,M, AU - Palazzo,P, AU - Camardella,L, AU - Tuppo,L, AU - Alessandri,C, AU - Breiteneder,H, AU - Ferreira,F, AU - Ciardiello,Maria A, AU - Mari,A, Y1 - 2011/02/11/ PY - 2011/2/12/entrez PY - 2011/2/12/pubmed PY - 2011/10/1/medline SP - 870 EP - 7 JF - Allergy JO - Allergy VL - 66 IS - 7 N2 - BACKGROUND: Kiwifruit is an important cause of food allergy. A high amount of a protein with a molecular mass compatible with that of Bet v 1 was observed in the kiwifruit extract. OBJECTIVE: To identify and characterize kirola, the 17-kDa protein of green kiwifruit (Act d 11). METHODS: Act d 11 was purified from green kiwifruit. Its primary structure was obtained by direct protein sequencing. The IgE binding was investigated by skin testing, immunoblotting, inhibition tests, and detection by the ISAC microarray in an Italian cohort and in selected Bet v 1-sensitized Austrian patients. A clinical evaluation of kiwi allergy was carried out. RESULTS: Act d 11 was identified as a member of the major latex protein/ripening-related protein (MLP/RRP) family. IgE binding to Act d 11 was shown by all the applied testing. Patients tested positive for Act d 11 and reporting symptoms on kiwifruit exposure were found within the Bet v 1-positive subset rather than within the population selected for highly reliable history of allergic reactions to kiwifruit. Epidemiology of Act d 11 IgE reactivity was documented in the two cohorts. IgE co-recognition of Act d 11 within the Bet v 1-like molecules is documented using the microarray IgE inhibition assay. CONCLUSIONS: Act d 11 is the first member of the MLP/RRP protein family to be described as an allergen. It displays IgE co-recognition with allergens belonging to the PR-10 family, including Bet v 1. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/21309790/Kiwifruit_Act_d_11_is_the_first_member_of_the_ripening_related_protein_family_identified_as_an_allergen_ L2 - https://doi.org/10.1111/j.1398-9995.2011.02555.x DB - PRIME DP - Unbound Medicine ER -