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Effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Jan; 36(1):27-33.ZN

Abstract

OBJECTIVE

To explore effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats (SHR) and the mechanisms underlying the protection against renal damage.

METHODS

Fifteen male SHRs (22 weeks old) were randomly divided into 3 groups (n=5 in each group): a SHR group, a fosinopril group [10 mg/(kg.d)], and a losartan group [50 mg/(kg.d)]. Age-matched Wistar-Kyoto (WKY) rats were chosen for a control group. Eight weeks later, tail arterial pressure, 24 hours urinary protein (Upro),urinary N-acetyl-β-D-glucosaminidase (NAGase) were measured. Renal pathological changes were examined under light microscopy by HE staining. The renal mRNA and protein expression of Klotho were determined by RT-PCR, immunohistochemical staining or Western blot. The levels of total antioxidant capacity (TAOC), malondialdehyde (MDA), Cu/Zn superoxide dismutase (Cu/Zn-SOD), Mn superoxide dismutase (Mn-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were determined.

RESULTS

The typical pathological characteristics of hypertensive renal damage were observed in the kidney of the SHR group.Compared with the SHR group, the systolic pressure, Upro, and urinary NAGase, the content of MDA and renal pathological damage was reduced while the renal Klotho expression and activities of TAOC, Cu/Zn-SOD, CAT, and GSH-Px were increased (P<0.05 or P<0.01) in the fosinopril or losartan group. There was no significant difference in renal Mn-SOD level among the 4 groups (P>0.05).

CONCLUSION

Fosinopril and losartan can exert protection against hypertensive renal damage through upregulating Klotho expression as well as reducing oxidative stress.

Authors+Show Affiliations

Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21311136

Citation

Tang, Rong, et al. "Effects of Fosinopril and Losartan On Renal Klotho Expression and Oxidative Stress in Spontaneously Hypertensive Rats." Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, vol. 36, no. 1, 2011, pp. 27-33.
Tang R, Zhou Q, Liu Z, et al. Effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011;36(1):27-33.
Tang, R., Zhou, Q., Liu, Z., Xiao, Z., & Pouranan, V. (2011). Effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats. Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, 36(1), 27-33. https://doi.org/10.3969/j.issn.1672-7347.2011.01.005
Tang R, et al. Effects of Fosinopril and Losartan On Renal Klotho Expression and Oxidative Stress in Spontaneously Hypertensive Rats. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011;36(1):27-33. PubMed PMID: 21311136.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats. AU - Tang,Rong, AU - Zhou,Qiaoling, AU - Liu,Zhichun, AU - Xiao,Zhou, AU - Pouranan,Veeraragoo, PY - 2011/2/12/entrez PY - 2011/2/12/pubmed PY - 2012/4/6/medline SP - 27 EP - 33 JF - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JO - Zhong Nan Da Xue Xue Bao Yi Xue Ban VL - 36 IS - 1 N2 - OBJECTIVE: To explore effects of fosinopril and losartan on renal Klotho expression and oxidative stress in spontaneously hypertensive rats (SHR) and the mechanisms underlying the protection against renal damage. METHODS: Fifteen male SHRs (22 weeks old) were randomly divided into 3 groups (n=5 in each group): a SHR group, a fosinopril group [10 mg/(kg.d)], and a losartan group [50 mg/(kg.d)]. Age-matched Wistar-Kyoto (WKY) rats were chosen for a control group. Eight weeks later, tail arterial pressure, 24 hours urinary protein (Upro),urinary N-acetyl-β-D-glucosaminidase (NAGase) were measured. Renal pathological changes were examined under light microscopy by HE staining. The renal mRNA and protein expression of Klotho were determined by RT-PCR, immunohistochemical staining or Western blot. The levels of total antioxidant capacity (TAOC), malondialdehyde (MDA), Cu/Zn superoxide dismutase (Cu/Zn-SOD), Mn superoxide dismutase (Mn-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were determined. RESULTS: The typical pathological characteristics of hypertensive renal damage were observed in the kidney of the SHR group.Compared with the SHR group, the systolic pressure, Upro, and urinary NAGase, the content of MDA and renal pathological damage was reduced while the renal Klotho expression and activities of TAOC, Cu/Zn-SOD, CAT, and GSH-Px were increased (P<0.05 or P<0.01) in the fosinopril or losartan group. There was no significant difference in renal Mn-SOD level among the 4 groups (P>0.05). CONCLUSION: Fosinopril and losartan can exert protection against hypertensive renal damage through upregulating Klotho expression as well as reducing oxidative stress. SN - 1672-7347 UR - https://www.unboundmedicine.com/medline/citation/21311136/Effects_of_fosinopril_and_losartan_on_renal_Klotho_expression_and_oxidative_stress_in_spontaneously_hypertensive_rats_ L2 - http://xbyxb.csu.edu.cn/xbwk/fileup/PDF/20110127.pdf DB - PRIME DP - Unbound Medicine ER -