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Proteinase-activated receptor 2 mediates thermal hyperalgesia and is upregulated in a rat model of chronic pancreatitis.
Pancreas. 2011 Mar; 40(2):300-7.P

Abstract

OBJECTIVES

The mechanism of pain in chronic pancreatitis (CP) has yet to be explored. Proteinase-activated receptor 2 (PAR2) plays a pronociceptive role in visceral pain. The study aimed to assess the expression of PAR2 in dorsal root ganglia (DRGs) and validate its role of thermal hyperalgesia in CP.

METHODS

Chronic pancreatitis model was induced by trinitrobenzene sulfonic acid infusion into rat pancreatic ducts. Abdominal hyperalgesia was measured by thermal withdrawal latencies. The expression of PAR2 and transient receptor potential vanilloid 1 (TRPV1) were analyzed by immunofluorescence and Western blot. The messenger RNA encoding PAR2 was quantitated by real-time polymerase chain reaction. The effects of short-term and long-term ulinastatin treatment on abdominal thermal hyperalgesia of rats with CP were measured.

RESULTS

Rats with CP showed a decreased thermal withdrawal latency. Proteinase-activated receptor 2 and TRPV1 were significantly upregulated in DRGs. The increased PAR2 protein expression was tightly correlated with thermal withdrawal latencies and TRPV1 expression. Short-term ulinastatin treatment inhibited the development of thermal hyperalgesia of rats with CP in a dose-dependent manner.

CONCLUSIONS

The thermal hyperalgesia in CP is associated with an up-regulation of the PAR2 in DRGs. Proteinase-activated receptor 2 was involved in the pain generation in rats with CP.

Authors+Show Affiliations

Division of Field Medicine, Department of Internal Medicine, Changhai Hospital, Second Military Medical University, Shanghai, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21311307

Citation

Zhang, Wei, et al. "Proteinase-activated Receptor 2 Mediates Thermal Hyperalgesia and Is Upregulated in a Rat Model of Chronic Pancreatitis." Pancreas, vol. 40, no. 2, 2011, pp. 300-7.
Zhang W, Gao J, Zhao T, et al. Proteinase-activated receptor 2 mediates thermal hyperalgesia and is upregulated in a rat model of chronic pancreatitis. Pancreas. 2011;40(2):300-7.
Zhang, W., Gao, J., Zhao, T., Wei, L., Wu, W., Bai, Y., Zou, D., & Li, Z. (2011). Proteinase-activated receptor 2 mediates thermal hyperalgesia and is upregulated in a rat model of chronic pancreatitis. Pancreas, 40(2), 300-7. https://doi.org/10.1097/MPA.0b013e318201cbc1
Zhang W, et al. Proteinase-activated Receptor 2 Mediates Thermal Hyperalgesia and Is Upregulated in a Rat Model of Chronic Pancreatitis. Pancreas. 2011;40(2):300-7. PubMed PMID: 21311307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteinase-activated receptor 2 mediates thermal hyperalgesia and is upregulated in a rat model of chronic pancreatitis. AU - Zhang,Wei, AU - Gao,Jun, AU - Zhao,Tao, AU - Wei,Lei, AU - Wu,Wenbin, AU - Bai,Yu, AU - Zou,Duowu, AU - Li,Zhaoshen, PY - 2011/2/12/entrez PY - 2011/2/12/pubmed PY - 2011/8/19/medline SP - 300 EP - 7 JF - Pancreas JO - Pancreas VL - 40 IS - 2 N2 - OBJECTIVES: The mechanism of pain in chronic pancreatitis (CP) has yet to be explored. Proteinase-activated receptor 2 (PAR2) plays a pronociceptive role in visceral pain. The study aimed to assess the expression of PAR2 in dorsal root ganglia (DRGs) and validate its role of thermal hyperalgesia in CP. METHODS: Chronic pancreatitis model was induced by trinitrobenzene sulfonic acid infusion into rat pancreatic ducts. Abdominal hyperalgesia was measured by thermal withdrawal latencies. The expression of PAR2 and transient receptor potential vanilloid 1 (TRPV1) were analyzed by immunofluorescence and Western blot. The messenger RNA encoding PAR2 was quantitated by real-time polymerase chain reaction. The effects of short-term and long-term ulinastatin treatment on abdominal thermal hyperalgesia of rats with CP were measured. RESULTS: Rats with CP showed a decreased thermal withdrawal latency. Proteinase-activated receptor 2 and TRPV1 were significantly upregulated in DRGs. The increased PAR2 protein expression was tightly correlated with thermal withdrawal latencies and TRPV1 expression. Short-term ulinastatin treatment inhibited the development of thermal hyperalgesia of rats with CP in a dose-dependent manner. CONCLUSIONS: The thermal hyperalgesia in CP is associated with an up-regulation of the PAR2 in DRGs. Proteinase-activated receptor 2 was involved in the pain generation in rats with CP. SN - 1536-4828 UR - https://www.unboundmedicine.com/medline/citation/21311307/Proteinase_activated_receptor_2_mediates_thermal_hyperalgesia_and_is_upregulated_in_a_rat_model_of_chronic_pancreatitis_ L2 - https://doi.org/10.1097/MPA.0b013e318201cbc1 DB - PRIME DP - Unbound Medicine ER -