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Coregulation of gene expression by sigma factors RpoE and RpoS in Salmonella enterica serovar Typhi during hyperosmotic stress.
Curr Microbiol. 2011 May; 62(5):1483-9.CM

Abstract

Salmonella enterica serovar Typhi (S. Typhi) is the cause of typhoid fever, a food-borne disease that is prevalent worldwide, most particularly in developing countries. RNA polymerase sigma factors RpoE (σ(E)) and RpoS (σ(S)) govern transcription initiation of two sets of genes in Escherichia and Salmonella. It was previously suggested that some genes might be coregulated by RpoE and RpoS in Salmonella under conditions of environmental stress, but experimental evidence has been lacking. We therefore constructed rpoS deletion (ΔrpoS) and double rpoE/rpoS deletion (ΔrpoE/ΔrpoS) mutants of S. Typhi and compared their growth properties with an rpoE mutant (ΔrpoE) and wild-type strains under conditions of hyperosmotic stress. We report that the ΔrpoE, ΔrpoS, and ΔrpoE/ΔrpoS strains grew more slowly under hyperosmotic stress conditions than the wild-type strain, and the ΔrpoE/ΔrpoS strain grew most slowly. The global transcriptional profiles of ΔrpoE, ΔrpoS, ΔrpoE/ΔrpoS after 30 min of hyperosmotic stress were investigated using a Salmonella genomic DNA microarray. The results of microarray indicated that the expression levels of 38 genes were markedly reduced during hyperosmotic stress in the double mutant ΔrpoE/ΔrpoS strain, but expression levels were not significantly affected by single ΔrpoE or ΔrpoS mutations. This was confirmed for several key genes by qRT-PCR. This study therefore indicated crosstalk between sigma factors RpoE and RpoS in S. Typhi under hyperosmotic conditions and provides new insights into the regulatory networks of S. Typhi.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Zhenjiang, China. hong_du@126.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21311887

Citation

Du, Hong, et al. "Coregulation of Gene Expression By Sigma Factors RpoE and RpoS in Salmonella Enterica Serovar Typhi During Hyperosmotic Stress." Current Microbiology, vol. 62, no. 5, 2011, pp. 1483-9.
Du H, Wang M, Luo Z, et al. Coregulation of gene expression by sigma factors RpoE and RpoS in Salmonella enterica serovar Typhi during hyperosmotic stress. Curr Microbiol. 2011;62(5):1483-9.
Du, H., Wang, M., Luo, Z., Ni, B., Wang, F., Meng, Y., Xu, S., & Huang, X. (2011). Coregulation of gene expression by sigma factors RpoE and RpoS in Salmonella enterica serovar Typhi during hyperosmotic stress. Current Microbiology, 62(5), 1483-9. https://doi.org/10.1007/s00284-011-9890-8
Du H, et al. Coregulation of Gene Expression By Sigma Factors RpoE and RpoS in Salmonella Enterica Serovar Typhi During Hyperosmotic Stress. Curr Microbiol. 2011;62(5):1483-9. PubMed PMID: 21311887.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coregulation of gene expression by sigma factors RpoE and RpoS in Salmonella enterica serovar Typhi during hyperosmotic stress. AU - Du,Hong, AU - Wang,Min, AU - Luo,Zhe, AU - Ni,Bin, AU - Wang,Fei, AU - Meng,Yanchen, AU - Xu,Shungao, AU - Huang,Xinxiang, Y1 - 2011/02/11/ PY - 2010/12/14/received PY - 2011/01/21/accepted PY - 2011/2/12/entrez PY - 2011/2/12/pubmed PY - 2011/7/13/medline SP - 1483 EP - 9 JF - Current microbiology JO - Curr Microbiol VL - 62 IS - 5 N2 - Salmonella enterica serovar Typhi (S. Typhi) is the cause of typhoid fever, a food-borne disease that is prevalent worldwide, most particularly in developing countries. RNA polymerase sigma factors RpoE (σ(E)) and RpoS (σ(S)) govern transcription initiation of two sets of genes in Escherichia and Salmonella. It was previously suggested that some genes might be coregulated by RpoE and RpoS in Salmonella under conditions of environmental stress, but experimental evidence has been lacking. We therefore constructed rpoS deletion (ΔrpoS) and double rpoE/rpoS deletion (ΔrpoE/ΔrpoS) mutants of S. Typhi and compared their growth properties with an rpoE mutant (ΔrpoE) and wild-type strains under conditions of hyperosmotic stress. We report that the ΔrpoE, ΔrpoS, and ΔrpoE/ΔrpoS strains grew more slowly under hyperosmotic stress conditions than the wild-type strain, and the ΔrpoE/ΔrpoS strain grew most slowly. The global transcriptional profiles of ΔrpoE, ΔrpoS, ΔrpoE/ΔrpoS after 30 min of hyperosmotic stress were investigated using a Salmonella genomic DNA microarray. The results of microarray indicated that the expression levels of 38 genes were markedly reduced during hyperosmotic stress in the double mutant ΔrpoE/ΔrpoS strain, but expression levels were not significantly affected by single ΔrpoE or ΔrpoS mutations. This was confirmed for several key genes by qRT-PCR. This study therefore indicated crosstalk between sigma factors RpoE and RpoS in S. Typhi under hyperosmotic conditions and provides new insights into the regulatory networks of S. Typhi. SN - 1432-0991 UR - https://www.unboundmedicine.com/medline/citation/21311887/Coregulation_of_gene_expression_by_sigma_factors_RpoE_and_RpoS_in_Salmonella_enterica_serovar_Typhi_during_hyperosmotic_stress_ L2 - https://dx.doi.org/10.1007/s00284-011-9890-8 DB - PRIME DP - Unbound Medicine ER -