Tags

Type your tag names separated by a space and hit enter

Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect.
J Sep Sci. 2011 Mar; 34(6):640-50.JS

Abstract

In the experiment, a high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry with selected reaction monitoring was used to simultaneously determine various classes of phytohormones, including indole-3-acetic acid, α-naphthaleneacetic acid, 2-chlorobenzoic acid, 4-chlorobenzoic acid, indole-3-butyric acid, gibberellic acid, 2,4-dichlorophenoxyacetic acid, 2-naphthoxyacetic acid, abscisic acid, 2,3,5-triiodobenzoic acid, uniconazole, paclobutrazol and 2,4-epibassinolide in rape tissues. The analyses were separated by an HPLC equipped with a reversed-phase column using a binary solvent system composed of methanol and water, both containing 0.1% of formic acid. The matrix effect was also considered and determined. The technology was applied to analyze rape tissues, including roots, stems, leaves, flowers, immature pods and rape seeds. The rape tissues were subjected to ultrasound-assisted extraction and purified by dispersive solid-phase extraction, and then transferred into the liquid chromatography system. The detection limit for each plant hormone was defined by the ratio of signal/background noise (S/N) of 3. The results showed perfect linearity (R(2) values of 0.9987-1.0000) and reproducibility of elution times (relative standard deviations, RSDs,<1%) and peak areas (RSDs,<7%) for all target compounds.

Authors+Show Affiliations

Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21312330

Citation

Fan, Sufang, et al. "Simultaneous Determination of 13 Phytohormones in Oilseed Rape Tissues By Liquid Chromatography-electrospray Tandem Mass Spectrometry and the Evaluation of the Matrix Effect." Journal of Separation Science, vol. 34, no. 6, 2011, pp. 640-50.
Fan S, Wang X, Li P, et al. Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect. J Sep Sci. 2011;34(6):640-50.
Fan, S., Wang, X., Li, P., Zhang, Q., & Zhang, W. (2011). Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect. Journal of Separation Science, 34(6), 640-50. https://doi.org/10.1002/jssc.201000541
Fan S, et al. Simultaneous Determination of 13 Phytohormones in Oilseed Rape Tissues By Liquid Chromatography-electrospray Tandem Mass Spectrometry and the Evaluation of the Matrix Effect. J Sep Sci. 2011;34(6):640-50. PubMed PMID: 21312330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of 13 phytohormones in oilseed rape tissues by liquid chromatography-electrospray tandem mass spectrometry and the evaluation of the matrix effect. AU - Fan,Sufang, AU - Wang,Xiupin, AU - Li,Peiwu, AU - Zhang,Qi, AU - Zhang,Wen, Y1 - 2011/02/11/ PY - 2010/07/22/received PY - 2010/11/26/revised PY - 2010/12/22/accepted PY - 2011/2/12/entrez PY - 2011/2/12/pubmed PY - 2011/7/13/medline SP - 640 EP - 50 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 6 N2 - In the experiment, a high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry with selected reaction monitoring was used to simultaneously determine various classes of phytohormones, including indole-3-acetic acid, α-naphthaleneacetic acid, 2-chlorobenzoic acid, 4-chlorobenzoic acid, indole-3-butyric acid, gibberellic acid, 2,4-dichlorophenoxyacetic acid, 2-naphthoxyacetic acid, abscisic acid, 2,3,5-triiodobenzoic acid, uniconazole, paclobutrazol and 2,4-epibassinolide in rape tissues. The analyses were separated by an HPLC equipped with a reversed-phase column using a binary solvent system composed of methanol and water, both containing 0.1% of formic acid. The matrix effect was also considered and determined. The technology was applied to analyze rape tissues, including roots, stems, leaves, flowers, immature pods and rape seeds. The rape tissues were subjected to ultrasound-assisted extraction and purified by dispersive solid-phase extraction, and then transferred into the liquid chromatography system. The detection limit for each plant hormone was defined by the ratio of signal/background noise (S/N) of 3. The results showed perfect linearity (R(2) values of 0.9987-1.0000) and reproducibility of elution times (relative standard deviations, RSDs,<1%) and peak areas (RSDs,<7%) for all target compounds. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21312330/Simultaneous_determination_of_13_phytohormones_in_oilseed_rape_tissues_by_liquid_chromatography_electrospray_tandem_mass_spectrometry_and_the_evaluation_of_the_matrix_effect_ L2 - https://doi.org/10.1002/jssc.201000541 DB - PRIME DP - Unbound Medicine ER -