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Immunosuppressive activity of florfenicol on the immune responses in mice.
Immunol Invest. 2011; 40(4):356-66.II

Abstract

Florfenicol is a new type of broad-spectrum antibacterial that has been used in veterinary clinics. It shows immunosuppressive activity on the immune responses to ovalbumin (OVA) in mice. In the present study, florfenicol suppressed lipopolysaccharide (LPS)-stimulated splenocyte proliferation in a concentration-dependent manner in vitro and in vivo. BALB/c mice were immunized subcutaneously with OVA on days 1 and 4. Following the second immunization, mice were treated with a single daily oral dose of florfenicol (50, 100, and 200 mg/kg) for 10 consecutive days. On day 14, blood samples were collected to analyze OVA-specific IgG, IgG1, and IgG2b antibodies, and splenocytes were harvested to assess lymphocyte proliferation, CD3(+) T and CD19(+) B lymphocyte subsets. The results presented here demonstrate that florfenicol not only significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation but also decreased the percentage of CD19(+) B cells in a dose-dependent manner and suppressed CD3(+) T cell at high doses. Moreover, OVA-specific IgG, IgG1 and IgG2b titers in OVA-immunized mice were reduced by florfenicol. These results suggest that florfenicol could suppress humoral and cellular immune responses in mice.

Authors+Show Affiliations

Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 30062, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21314266

Citation

Shuang, Guan, et al. "Immunosuppressive Activity of Florfenicol On the Immune Responses in Mice." Immunological Investigations, vol. 40, no. 4, 2011, pp. 356-66.
Shuang G, Yu S, Weixiao G, et al. Immunosuppressive activity of florfenicol on the immune responses in mice. Immunol Invest. 2011;40(4):356-66.
Shuang, G., Yu, S., Weixiao, G., Dacheng, W., Zhichao, Z., Jing, L., & Xuming, D. (2011). Immunosuppressive activity of florfenicol on the immune responses in mice. Immunological Investigations, 40(4), 356-66. https://doi.org/10.3109/08820139.2010.551434
Shuang G, et al. Immunosuppressive Activity of Florfenicol On the Immune Responses in Mice. Immunol Invest. 2011;40(4):356-66. PubMed PMID: 21314266.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosuppressive activity of florfenicol on the immune responses in mice. AU - Shuang,Guan, AU - Yu,Song, AU - Weixiao,Guo, AU - Dacheng,Wang, AU - Zhichao,Zhang, AU - Jing,Lu, AU - Xuming,Deng, Y1 - 2011/02/11/ PY - 2011/2/15/entrez PY - 2011/2/15/pubmed PY - 2011/8/5/medline SP - 356 EP - 66 JF - Immunological investigations JO - Immunol Invest VL - 40 IS - 4 N2 - Florfenicol is a new type of broad-spectrum antibacterial that has been used in veterinary clinics. It shows immunosuppressive activity on the immune responses to ovalbumin (OVA) in mice. In the present study, florfenicol suppressed lipopolysaccharide (LPS)-stimulated splenocyte proliferation in a concentration-dependent manner in vitro and in vivo. BALB/c mice were immunized subcutaneously with OVA on days 1 and 4. Following the second immunization, mice were treated with a single daily oral dose of florfenicol (50, 100, and 200 mg/kg) for 10 consecutive days. On day 14, blood samples were collected to analyze OVA-specific IgG, IgG1, and IgG2b antibodies, and splenocytes were harvested to assess lymphocyte proliferation, CD3(+) T and CD19(+) B lymphocyte subsets. The results presented here demonstrate that florfenicol not only significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation but also decreased the percentage of CD19(+) B cells in a dose-dependent manner and suppressed CD3(+) T cell at high doses. Moreover, OVA-specific IgG, IgG1 and IgG2b titers in OVA-immunized mice were reduced by florfenicol. These results suggest that florfenicol could suppress humoral and cellular immune responses in mice. SN - 1532-4311 UR - https://www.unboundmedicine.com/medline/citation/21314266/Immunosuppressive_activity_of_florfenicol_on_the_immune_responses_in_mice_ L2 - https://www.tandfonline.com/doi/full/10.3109/08820139.2010.551434 DB - PRIME DP - Unbound Medicine ER -