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Visfatin stimulates a cellular renin-angiotensin system in cultured rat mesangial cells.
Endocr Res. 2011; 36(3):93-100.ER

Abstract

OBJECTIVE

Visfatin is a newly identified proinflammatory adipocytokine whose plasma levels have been reported to be higher in subjects with type 2 diabetes mellitus. Recent studies have shown that visfatin increases the synthesis of profibrotic molecules in mesangial cells (MCs) and thus plays an important role in the pathogenesis of diabetic nephropathy. However, the mechanism by which visfatin induces kidney injury is unknown. The renin-angiotensin system (RAS) plays pivotal roles in renal diseases. Therefore, in this study the effect of visfatin on the regulation of RAS in MCs was examined.

METHODS

Cultured rat MCs were treated with different doses of visfatin. We used real-time polymerase chain reaction to detect mRNA expression of renin, angiotensinogen (AGT), angiotensin-converting enzyme (ACE), angiotensin II (Ang II) type 1 receptor (AT1), and Ang II type 2 receptor (AT2); western blot analysis for expression of ANG and AT1; and radioimmunoassay to measure Ang II production from MCs in the supernatants of culture media.

RESULTS

Visfatin treatments increased renin, angiotensinogen (AGT), AT1 mRNA, and AGT, AT1 protein expression, as well as Ang II levels in a dose-dependent manner but did not affect ACE and AT2 mRNA levels in cultured rat MCs.

CONCLUSIONS

Our findings suggest that visfatin imparts a detrimental effect on diabetic nephropathy at least partly through the activation of intrarenal RAS.

Authors+Show Affiliations

Department of Endocrinology, The Second Affiliated Hospital, Sun Yat-Sen University, Guangzhou, PR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21314328

Citation

Huang, Qiong, et al. "Visfatin Stimulates a Cellular Renin-angiotensin System in Cultured Rat Mesangial Cells." Endocrine Research, vol. 36, no. 3, 2011, pp. 93-100.
Huang Q, Guo Y, Zeng H, et al. Visfatin stimulates a cellular renin-angiotensin system in cultured rat mesangial cells. Endocr Res. 2011;36(3):93-100.
Huang, Q., Guo, Y., Zeng, H., Xie, W., Yan, H., & Ding, H. (2011). Visfatin stimulates a cellular renin-angiotensin system in cultured rat mesangial cells. Endocrine Research, 36(3), 93-100. https://doi.org/10.3109/07435800.2010.539992
Huang Q, et al. Visfatin Stimulates a Cellular Renin-angiotensin System in Cultured Rat Mesangial Cells. Endocr Res. 2011;36(3):93-100. PubMed PMID: 21314328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Visfatin stimulates a cellular renin-angiotensin system in cultured rat mesangial cells. AU - Huang,Qiong, AU - Guo,Ying, AU - Zeng,Hua, AU - Xie,Wenfeng, AU - Yan,Haiyan, AU - Ding,Helin, Y1 - 2011/02/11/ PY - 2011/2/15/entrez PY - 2011/2/15/pubmed PY - 2011/11/1/medline SP - 93 EP - 100 JF - Endocrine research JO - Endocr. Res. VL - 36 IS - 3 N2 - OBJECTIVE: Visfatin is a newly identified proinflammatory adipocytokine whose plasma levels have been reported to be higher in subjects with type 2 diabetes mellitus. Recent studies have shown that visfatin increases the synthesis of profibrotic molecules in mesangial cells (MCs) and thus plays an important role in the pathogenesis of diabetic nephropathy. However, the mechanism by which visfatin induces kidney injury is unknown. The renin-angiotensin system (RAS) plays pivotal roles in renal diseases. Therefore, in this study the effect of visfatin on the regulation of RAS in MCs was examined. METHODS: Cultured rat MCs were treated with different doses of visfatin. We used real-time polymerase chain reaction to detect mRNA expression of renin, angiotensinogen (AGT), angiotensin-converting enzyme (ACE), angiotensin II (Ang II) type 1 receptor (AT1), and Ang II type 2 receptor (AT2); western blot analysis for expression of ANG and AT1; and radioimmunoassay to measure Ang II production from MCs in the supernatants of culture media. RESULTS: Visfatin treatments increased renin, angiotensinogen (AGT), AT1 mRNA, and AGT, AT1 protein expression, as well as Ang II levels in a dose-dependent manner but did not affect ACE and AT2 mRNA levels in cultured rat MCs. CONCLUSIONS: Our findings suggest that visfatin imparts a detrimental effect on diabetic nephropathy at least partly through the activation of intrarenal RAS. SN - 1532-4206 UR - https://www.unboundmedicine.com/medline/citation/21314328/Visfatin_stimulates_a_cellular_renin_angiotensin_system_in_cultured_rat_mesangial_cells_ L2 - http://www.tandfonline.com/doi/full/10.3109/07435800.2010.539992 DB - PRIME DP - Unbound Medicine ER -