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Silver nanoparticles alter zebrafish development and larval behavior: distinct roles for particle size, coating and composition.
Neurotoxicol Teratol. 2011 Nov-Dec; 33(6):708-14.NT

Abstract

Silver nanoparticles (AgNPs) act as antibacterials by releasing monovalent silver (Ag(+)) and are increasingly used in consumer products, thus elevating exposures in human and wildlife populations. In vitro models indicate that AgNPs are likely to be developmental neurotoxicants with actions distinct from those of Ag(+). We exposed developing zebrafish (Danio rerio) to Ag(+) or AgNPs on days 0-5 post-fertilization and evaluated hatching, morphology, survival and swim bladder inflation. Larval swimming behavior and responses to different lighting conditions were assessed 24h after the termination of exposure. Comparisons were made with AgNPs of different sizes and coatings: 10nm citrate-coated AgNP (AgNP-C), and 10 or 50nm polyvinylpyrrolidone-coated AgNPs (AgNP-PVP). Ag(+) and AgNP-C delayed hatching to a similar extent but Ag(+) was more effective in slowing swim bladder inflation, and elicited greater dysmorphology and mortality. In behavioral assessments, Ag(+) exposed fish were hyperresponsive to light changes, whereas AgNP-C exposed fish showed normal responses. Neither of the AgNP-PVPs affected survival or morphology but both evoked significant changes in swimming responses to light in ways that were distinct from Ag(+) and each other. The smaller AgNP-PVP caused overall hypoactivity whereas the larger caused hyperactivity. AgNPs are less potent than Ag(+) with respect to dysmorphology and loss of viability, but nevertheless produce neurobehavioral effects that highly depend on particle coating and size, rather than just reflecting the release of Ag(+). Different AgNP formulations are thus likely to produce distinct patterns of developmental neurotoxicity.

Authors+Show Affiliations

Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21315816

Citation

Powers, Christina M., et al. "Silver Nanoparticles Alter Zebrafish Development and Larval Behavior: Distinct Roles for Particle Size, Coating and Composition." Neurotoxicology and Teratology, vol. 33, no. 6, 2011, pp. 708-14.
Powers CM, Slotkin TA, Seidler FJ, et al. Silver nanoparticles alter zebrafish development and larval behavior: distinct roles for particle size, coating and composition. Neurotoxicol Teratol. 2011;33(6):708-14.
Powers, C. M., Slotkin, T. A., Seidler, F. J., Badireddy, A. R., & Padilla, S. (2011). Silver nanoparticles alter zebrafish development and larval behavior: distinct roles for particle size, coating and composition. Neurotoxicology and Teratology, 33(6), 708-14. https://doi.org/10.1016/j.ntt.2011.02.002
Powers CM, et al. Silver Nanoparticles Alter Zebrafish Development and Larval Behavior: Distinct Roles for Particle Size, Coating and Composition. Neurotoxicol Teratol. 2011 Nov-Dec;33(6):708-14. PubMed PMID: 21315816.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanoparticles alter zebrafish development and larval behavior: distinct roles for particle size, coating and composition. AU - Powers,Christina M, AU - Slotkin,Theodore A, AU - Seidler,Frederic J, AU - Badireddy,Appala R, AU - Padilla,Stephanie, Y1 - 2011/02/22/ PY - 2010/12/14/received PY - 2011/02/04/revised PY - 2011/02/04/accepted PY - 2011/2/15/entrez PY - 2011/2/15/pubmed PY - 2012/5/15/medline SP - 708 EP - 14 JF - Neurotoxicology and teratology JO - Neurotoxicol Teratol VL - 33 IS - 6 N2 - Silver nanoparticles (AgNPs) act as antibacterials by releasing monovalent silver (Ag(+)) and are increasingly used in consumer products, thus elevating exposures in human and wildlife populations. In vitro models indicate that AgNPs are likely to be developmental neurotoxicants with actions distinct from those of Ag(+). We exposed developing zebrafish (Danio rerio) to Ag(+) or AgNPs on days 0-5 post-fertilization and evaluated hatching, morphology, survival and swim bladder inflation. Larval swimming behavior and responses to different lighting conditions were assessed 24h after the termination of exposure. Comparisons were made with AgNPs of different sizes and coatings: 10nm citrate-coated AgNP (AgNP-C), and 10 or 50nm polyvinylpyrrolidone-coated AgNPs (AgNP-PVP). Ag(+) and AgNP-C delayed hatching to a similar extent but Ag(+) was more effective in slowing swim bladder inflation, and elicited greater dysmorphology and mortality. In behavioral assessments, Ag(+) exposed fish were hyperresponsive to light changes, whereas AgNP-C exposed fish showed normal responses. Neither of the AgNP-PVPs affected survival or morphology but both evoked significant changes in swimming responses to light in ways that were distinct from Ag(+) and each other. The smaller AgNP-PVP caused overall hypoactivity whereas the larger caused hyperactivity. AgNPs are less potent than Ag(+) with respect to dysmorphology and loss of viability, but nevertheless produce neurobehavioral effects that highly depend on particle coating and size, rather than just reflecting the release of Ag(+). Different AgNP formulations are thus likely to produce distinct patterns of developmental neurotoxicity. SN - 1872-9738 UR - https://www.unboundmedicine.com/medline/citation/21315816/Silver_nanoparticles_alter_zebrafish_development_and_larval_behavior:_distinct_roles_for_particle_size_coating_and_composition_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0892-0362(11)00009-2 DB - PRIME DP - Unbound Medicine ER -