Tags

Type your tag names separated by a space and hit enter

Quality control of Toxoplasma gondii in meat packages: standardization of an ELISA test and its use for detection in rabbit meat cuts.
Meat Sci 2011; 88(3):584-9MS

Abstract

Toxoplasma gondii causes severe disease both to man and livestock and its detection in meat after slaughtering requires PCR or biological tests. Meat packages contain retained exudate that could be used for serology due to its blood content. Similar studies reported false negative assays in those tests. We standardized an anti-T. gondii IgG ELISA in muscle juices from experimentally infected rabbits, with blood content determination by cyanhemoglobin spectrophotometry. IgG titers and immunoblotting profiles were similar in blood, serum or meat juice, after blood content correction. These assays were adequate regardless of the storage time up to 120 days or freeze-thaw cycles, without false negative results. We also found 1.35% (1/74) positive sample in commercial Brazilian rabbit meat cuts, by this assay. The blood content determination shows ELISA of meat juice may be useful for quality control for toxoplasmosis monitoring.

Authors+Show Affiliations

Laboratório de Protozoologia do Instituto de Medicina Tropical da Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470, 05403-000 São Paulo, SP, Brazil. juliana.mecca@usp.brNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21316867

Citation

Mecca, Juliana Nunes, et al. "Quality Control of Toxoplasma Gondii in Meat Packages: Standardization of an ELISA Test and Its Use for Detection in Rabbit Meat Cuts." Meat Science, vol. 88, no. 3, 2011, pp. 584-9.
Mecca JN, Meireles LR, de Andrade HF. Quality control of Toxoplasma gondii in meat packages: standardization of an ELISA test and its use for detection in rabbit meat cuts. Meat Sci. 2011;88(3):584-9.
Mecca, J. N., Meireles, L. R., & de Andrade, H. F. (2011). Quality control of Toxoplasma gondii in meat packages: standardization of an ELISA test and its use for detection in rabbit meat cuts. Meat Science, 88(3), pp. 584-9. doi:10.1016/j.meatsci.2011.01.016.
Mecca JN, Meireles LR, de Andrade HF. Quality Control of Toxoplasma Gondii in Meat Packages: Standardization of an ELISA Test and Its Use for Detection in Rabbit Meat Cuts. Meat Sci. 2011;88(3):584-9. PubMed PMID: 21316867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quality control of Toxoplasma gondii in meat packages: standardization of an ELISA test and its use for detection in rabbit meat cuts. AU - Mecca,Juliana Nunes, AU - Meireles,Luciana Regina, AU - de Andrade,Heitor Franco,Jr Y1 - 2011/01/27/ PY - 2010/06/25/received PY - 2011/01/18/revised PY - 2011/01/20/accepted PY - 2011/2/15/entrez PY - 2011/2/15/pubmed PY - 2011/8/2/medline SP - 584 EP - 9 JF - Meat science JO - Meat Sci. VL - 88 IS - 3 N2 - Toxoplasma gondii causes severe disease both to man and livestock and its detection in meat after slaughtering requires PCR or biological tests. Meat packages contain retained exudate that could be used for serology due to its blood content. Similar studies reported false negative assays in those tests. We standardized an anti-T. gondii IgG ELISA in muscle juices from experimentally infected rabbits, with blood content determination by cyanhemoglobin spectrophotometry. IgG titers and immunoblotting profiles were similar in blood, serum or meat juice, after blood content correction. These assays were adequate regardless of the storage time up to 120 days or freeze-thaw cycles, without false negative results. We also found 1.35% (1/74) positive sample in commercial Brazilian rabbit meat cuts, by this assay. The blood content determination shows ELISA of meat juice may be useful for quality control for toxoplasmosis monitoring. SN - 1873-4138 UR - https://www.unboundmedicine.com/medline/citation/21316867/Quality_control_of_Toxoplasma_gondii_in_meat_packages:_standardization_of_an_ELISA_test_and_its_use_for_detection_in_rabbit_meat_cuts L2 - https://linkinghub.elsevier.com/retrieve/pii/S0309-1740(11)00029-5 DB - PRIME DP - Unbound Medicine ER -