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Inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (Mangifera indica L.).
Appl Environ Microbiol. 2011 Apr; 77(7):2215-23.AE

Abstract

This study investigated the antimicrobial activities and modes of action of penta-, hexa-, hepta-, octa-, nona-, and deca-O-galloylglucose (gallotannins) isolated from mango kernels. The MICs and minimum bactericidal concentrations (MBCs) against food-borne bacteria and fungi were determined using a critical dilution assay. Gram-positive bacteria were generally more susceptible to gallotannins than were Gram-negative bacteria. The MICs of gallotannins against Bacillus subtilis, Bacillus cereus, Clostridium botulinum, Campylobacter jejuni, Listeria monocytogenes, and Staphylococcus aureus were 0.2 g liter(-1) or less; enterotoxigenic Escherichia coli and Salmonella enterica were inhibited by 0.5 to 1 g liter(-1), and lactic acid bacteria were resistant. The use of lipopolysaccharide mutants of S. enterica indicated that the outer membrane confers resistance toward gallotannins. Supplementation of LB medium with iron eliminated the inhibitory activity of gallotannins against Staphylococcus aureus, and siderophore-deficient mutants of S. enterica were less resistant toward gallotannins than was the wild-type strain. Hepta-O-galloylglucose sensitized Lactobacillus plantarum TMW1.460 to hop extract, indicating inactivation of hop resistance mechanisms, e.g., the multidrug resistance (MDR) transporter HorA. Carbohydrate metabolism of Lactococcus lactis MG1363, a conditionally respiring organism, was influenced by hepta-O-galloylglucose when grown under aerobic conditions and in the presence of heme but not under anaerobic conditions, indicating that gallotannins influence the respiratory chain. In conclusion, the inhibitory activities of gallotannins are attributable to their strong affinity for iron and likely additionally relate to the inactivation of membrane-bound proteins.

Authors+Show Affiliations

University of Alberta, Department of Agricultural, Food, and Nutritional Science, 4-10 Ag/For, Edmonton, AB T6G 2P5, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21317249

Citation

Engels, Christina, et al. "Inhibitory Spectra and Modes of Antimicrobial Action of Gallotannins From Mango Kernels (Mangifera Indica L.)." Applied and Environmental Microbiology, vol. 77, no. 7, 2011, pp. 2215-23.
Engels C, Schieber A, Gänzle MG. Inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (Mangifera indica L.). Appl Environ Microbiol. 2011;77(7):2215-23.
Engels, C., Schieber, A., & Gänzle, M. G. (2011). Inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (Mangifera indica L.). Applied and Environmental Microbiology, 77(7), 2215-23. https://doi.org/10.1128/AEM.02521-10
Engels C, Schieber A, Gänzle MG. Inhibitory Spectra and Modes of Antimicrobial Action of Gallotannins From Mango Kernels (Mangifera Indica L.). Appl Environ Microbiol. 2011;77(7):2215-23. PubMed PMID: 21317249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (Mangifera indica L.). AU - Engels,Christina, AU - Schieber,Andreas, AU - Gänzle,Michael G, Y1 - 2011/02/11/ PY - 2011/2/15/entrez PY - 2011/2/15/pubmed PY - 2011/7/19/medline SP - 2215 EP - 23 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 77 IS - 7 N2 - This study investigated the antimicrobial activities and modes of action of penta-, hexa-, hepta-, octa-, nona-, and deca-O-galloylglucose (gallotannins) isolated from mango kernels. The MICs and minimum bactericidal concentrations (MBCs) against food-borne bacteria and fungi were determined using a critical dilution assay. Gram-positive bacteria were generally more susceptible to gallotannins than were Gram-negative bacteria. The MICs of gallotannins against Bacillus subtilis, Bacillus cereus, Clostridium botulinum, Campylobacter jejuni, Listeria monocytogenes, and Staphylococcus aureus were 0.2 g liter(-1) or less; enterotoxigenic Escherichia coli and Salmonella enterica were inhibited by 0.5 to 1 g liter(-1), and lactic acid bacteria were resistant. The use of lipopolysaccharide mutants of S. enterica indicated that the outer membrane confers resistance toward gallotannins. Supplementation of LB medium with iron eliminated the inhibitory activity of gallotannins against Staphylococcus aureus, and siderophore-deficient mutants of S. enterica were less resistant toward gallotannins than was the wild-type strain. Hepta-O-galloylglucose sensitized Lactobacillus plantarum TMW1.460 to hop extract, indicating inactivation of hop resistance mechanisms, e.g., the multidrug resistance (MDR) transporter HorA. Carbohydrate metabolism of Lactococcus lactis MG1363, a conditionally respiring organism, was influenced by hepta-O-galloylglucose when grown under aerobic conditions and in the presence of heme but not under anaerobic conditions, indicating that gallotannins influence the respiratory chain. In conclusion, the inhibitory activities of gallotannins are attributable to their strong affinity for iron and likely additionally relate to the inactivation of membrane-bound proteins. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/21317249/Inhibitory_spectra_and_modes_of_antimicrobial_action_of_gallotannins_from_mango_kernels__Mangifera_indica_L___ L2 - https://journals.asm.org/doi/10.1128/AEM.02521-10?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -