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Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene.
Microb Pathog. 2011 Jun; 50(6):303-13.MP

Abstract

Salmonella enterica serovar Typhimurium is a frequent cause of enteric disease due to the consumption of contaminated food. Identification and characterization of bacterial factors involved in Salmonella pathogenesis would help develop effective strategies for controlling salmonellosis. To investigate the role of glucose-inhibited division gene (gidA) in Salmonella virulence, we constructed a Salmonella mutant strain in which gidA was deleted. Deletion of gidA rendered Salmonella deficient in the invasion of intestinal epithelial cells, bacterial motility, intracellular survival, and induction of cytotoxicity in host cells. Deletion of gidA rendered the organism to display a filamentous morphology compared to the normal rod-shaped nature of Salmonella. Furthermore, a significant attenuation in the induction of inflammatory cytokines and chemokines, histopathological lesions, and systemic infection was observed in mice infected with the gidA mutant. Most importantly, a significant increase in LD(50) was observed in mice infected with the gidA mutant, and mice immunized with the gidA mutant were able to survive a lethal dose of wild-type Salmonella. Additionally, deletion of gidA significantly altered the expression of several bacterial factors associated with pathogenesis as indicated by global transcriptional and proteomic profiling. Taken together, our data indicate GidA as a potential regulator of Salmonella virulence genes.

Authors+Show Affiliations

Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Dr., Madison, WI 53706, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21320585

Citation

Shippy, Daniel C., et al. "Biological and Virulence Characteristics of Salmonella Enterica Serovar Typhimurium Following Deletion of Glucose-inhibited Division (gidA) Gene." Microbial Pathogenesis, vol. 50, no. 6, 2011, pp. 303-13.
Shippy DC, Eakley NM, Bochsler PN, et al. Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene. Microb Pathog. 2011;50(6):303-13.
Shippy, D. C., Eakley, N. M., Bochsler, P. N., Chopra, A. K., & Fadl, A. A. (2011). Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene. Microbial Pathogenesis, 50(6), 303-13. https://doi.org/10.1016/j.micpath.2011.02.004
Shippy DC, et al. Biological and Virulence Characteristics of Salmonella Enterica Serovar Typhimurium Following Deletion of Glucose-inhibited Division (gidA) Gene. Microb Pathog. 2011;50(6):303-13. PubMed PMID: 21320585.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene. AU - Shippy,Daniel C, AU - Eakley,Nicholas M, AU - Bochsler,Philip N, AU - Chopra,Ashok K, AU - Fadl,Amin A, Y1 - 2011/02/12/ PY - 2010/10/23/received PY - 2011/01/29/revised PY - 2011/02/04/accepted PY - 2011/2/16/entrez PY - 2011/2/16/pubmed PY - 2011/8/19/medline SP - 303 EP - 13 JF - Microbial pathogenesis JO - Microb Pathog VL - 50 IS - 6 N2 - Salmonella enterica serovar Typhimurium is a frequent cause of enteric disease due to the consumption of contaminated food. Identification and characterization of bacterial factors involved in Salmonella pathogenesis would help develop effective strategies for controlling salmonellosis. To investigate the role of glucose-inhibited division gene (gidA) in Salmonella virulence, we constructed a Salmonella mutant strain in which gidA was deleted. Deletion of gidA rendered Salmonella deficient in the invasion of intestinal epithelial cells, bacterial motility, intracellular survival, and induction of cytotoxicity in host cells. Deletion of gidA rendered the organism to display a filamentous morphology compared to the normal rod-shaped nature of Salmonella. Furthermore, a significant attenuation in the induction of inflammatory cytokines and chemokines, histopathological lesions, and systemic infection was observed in mice infected with the gidA mutant. Most importantly, a significant increase in LD(50) was observed in mice infected with the gidA mutant, and mice immunized with the gidA mutant were able to survive a lethal dose of wild-type Salmonella. Additionally, deletion of gidA significantly altered the expression of several bacterial factors associated with pathogenesis as indicated by global transcriptional and proteomic profiling. Taken together, our data indicate GidA as a potential regulator of Salmonella virulence genes. SN - 1096-1208 UR - https://www.unboundmedicine.com/medline/citation/21320585/Biological_and_virulence_characteristics_of_Salmonella_enterica_serovar_Typhimurium_following_deletion_of_glucose_inhibited_division__gidA__gene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0882-4010(11)00028-3 DB - PRIME DP - Unbound Medicine ER -