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Antioxidant response and oxidative stress levels in Macrobrachium borellii (Crustacea: Palaemonidae) exposed to the water-soluble fraction of petroleum.
Comp Biochem Physiol C Toxicol Pharmacol. 2011 May; 153(4):415-21.CB

Abstract

The aim of the present work was to evaluate the effect of the water soluble fraction of hydrocarbons (WSF) on the antioxidant status of the freshwater prawn Macrobrachium borellii. First, seasonal variations were studied in a non-polluted area. Hepatopancreas and gills showed season-related fluctuations in catalase (CAT), glutathione-S-transferase (GST) activities and in lipid peroxidation levels (LPO), but not in superoxide dismutase (SOD). Then, adults were exposed semi-statically to sublethal doses for 7days. CAT, SOD, GST, and glutathione peroxidase (GPx) activities and LPO, reduced glutathione (GSH) and protein oxidation (PO) levels were determined. Exposed individuals showed significant increases in CAT, SOD, and GST activities in hepatopancreas and CAT activity in gills. GPx activity did not vary in either tissues. While LPO levels increased, GSH levels decreased significantly in hepatopancreas of exposed animals, but PO levels showed no variation. Induction of SOD was also assessed by Real-time PCR mRNA expression in hepatopancreas. The non-enzymatic antioxidant activity was also tested; ABTS 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) was higher in hemolymph of treated-prawns compared to controls, but ferric reducing activity of plasma assay (FRAP) values did not change. Taken together, the present results indicated that the antioxidant defenses of M. borellii, mainly in hepatopancreas, were significantly affected by aquatic hydrocarbon contamination, regardless of the season.

Authors+Show Affiliations

Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP) CONICET CCT La Plata-Universidad Nacional de La Plata (UNLP), calles 60 y 120, (1900) La Plata, Argentina. sabrinalavarias@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21320634

Citation

Lavarías, S, et al. "Antioxidant Response and Oxidative Stress Levels in Macrobrachium Borellii (Crustacea: Palaemonidae) Exposed to the Water-soluble Fraction of Petroleum." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 153, no. 4, 2011, pp. 415-21.
Lavarías S, Heras H, Pedrini N, et al. Antioxidant response and oxidative stress levels in Macrobrachium borellii (Crustacea: Palaemonidae) exposed to the water-soluble fraction of petroleum. Comp Biochem Physiol C Toxicol Pharmacol. 2011;153(4):415-21.
Lavarías, S., Heras, H., Pedrini, N., Tournier, H., & Ansaldo, M. (2011). Antioxidant response and oxidative stress levels in Macrobrachium borellii (Crustacea: Palaemonidae) exposed to the water-soluble fraction of petroleum. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 153(4), 415-21. https://doi.org/10.1016/j.cbpc.2011.02.002
Lavarías S, et al. Antioxidant Response and Oxidative Stress Levels in Macrobrachium Borellii (Crustacea: Palaemonidae) Exposed to the Water-soluble Fraction of Petroleum. Comp Biochem Physiol C Toxicol Pharmacol. 2011;153(4):415-21. PubMed PMID: 21320634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant response and oxidative stress levels in Macrobrachium borellii (Crustacea: Palaemonidae) exposed to the water-soluble fraction of petroleum. AU - Lavarías,S, AU - Heras,H, AU - Pedrini,N, AU - Tournier,H, AU - Ansaldo,M, Y1 - 2011/02/12/ PY - 2010/12/21/received PY - 2011/02/07/revised PY - 2011/02/07/accepted PY - 2011/2/16/entrez PY - 2011/2/16/pubmed PY - 2011/7/16/medline SP - 415 EP - 21 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp. Biochem. Physiol. C Toxicol. Pharmacol. VL - 153 IS - 4 N2 - The aim of the present work was to evaluate the effect of the water soluble fraction of hydrocarbons (WSF) on the antioxidant status of the freshwater prawn Macrobrachium borellii. First, seasonal variations were studied in a non-polluted area. Hepatopancreas and gills showed season-related fluctuations in catalase (CAT), glutathione-S-transferase (GST) activities and in lipid peroxidation levels (LPO), but not in superoxide dismutase (SOD). Then, adults were exposed semi-statically to sublethal doses for 7days. CAT, SOD, GST, and glutathione peroxidase (GPx) activities and LPO, reduced glutathione (GSH) and protein oxidation (PO) levels were determined. Exposed individuals showed significant increases in CAT, SOD, and GST activities in hepatopancreas and CAT activity in gills. GPx activity did not vary in either tissues. While LPO levels increased, GSH levels decreased significantly in hepatopancreas of exposed animals, but PO levels showed no variation. Induction of SOD was also assessed by Real-time PCR mRNA expression in hepatopancreas. The non-enzymatic antioxidant activity was also tested; ABTS 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) was higher in hemolymph of treated-prawns compared to controls, but ferric reducing activity of plasma assay (FRAP) values did not change. Taken together, the present results indicated that the antioxidant defenses of M. borellii, mainly in hepatopancreas, were significantly affected by aquatic hydrocarbon contamination, regardless of the season. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/21320634/Antioxidant_response_and_oxidative_stress_levels_in_Macrobrachium_borellii__Crustacea:_Palaemonidae__exposed_to_the_water_soluble_fraction_of_petroleum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(11)00018-4 DB - PRIME DP - Unbound Medicine ER -