Tags

Type your tag names separated by a space and hit enter

Pax7-FKHR transcriptional activity is enhanced by transcriptionally repressed MyoD.
J Cell Biochem. 2011 May; 112(5):1410-7.JC

Abstract

Alveolar rhabdomyosarcoma (ARMS) are characterized by the expression of chimeric transcription factors Pax3-FKHR and Pax7-FKHR, due to chromosomal translocations fusing PAX3 or PAX7 with the FKHR gene. Although ARMS exhibits a muscle lineage phenotype, the cells evade terminal differentiation despite expressing the potent myogenic transcriptional regulator MyoD. Here we show that while Pax7-FKHR inhibits MyoD-dependent transcription, MyoD enhances Pax7-FKHR activity in myogenic cell cultures. Importantly, this effect is not recapitulated by close related transcription factor myogenin and involves specific MyoD functional domains, distinct from those required for Pax7 to regulate MyoD during muscle formation. Together, these results suggest that although repressed as a myogenic regulatory factor, MyoD can play an active role in ARMS by augmenting Pax7-FKHR function.

Authors+Show Affiliations

Facultad de Ciencias Biológicas, Departamento Biología Celular y Molecular, Pontificia Universidad Católica de Chile, Alameda 340, Santiago, Chile. holguin@bio.puc.clNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21321994

Citation

Olguín, Hugo C., et al. "Pax7-FKHR Transcriptional Activity Is Enhanced By Transcriptionally Repressed MyoD." Journal of Cellular Biochemistry, vol. 112, no. 5, 2011, pp. 1410-7.
Olguín HC, Patzlaff NE, Olwin BB. Pax7-FKHR transcriptional activity is enhanced by transcriptionally repressed MyoD. J Cell Biochem. 2011;112(5):1410-7.
Olguín, H. C., Patzlaff, N. E., & Olwin, B. B. (2011). Pax7-FKHR transcriptional activity is enhanced by transcriptionally repressed MyoD. Journal of Cellular Biochemistry, 112(5), 1410-7. https://doi.org/10.1002/jcb.23057
Olguín HC, Patzlaff NE, Olwin BB. Pax7-FKHR Transcriptional Activity Is Enhanced By Transcriptionally Repressed MyoD. J Cell Biochem. 2011;112(5):1410-7. PubMed PMID: 21321994.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pax7-FKHR transcriptional activity is enhanced by transcriptionally repressed MyoD. AU - Olguín,Hugo C, AU - Patzlaff,Natalie E, AU - Olwin,Bradley B, PY - 2011/2/16/entrez PY - 2011/2/16/pubmed PY - 2011/7/21/medline SP - 1410 EP - 7 JF - Journal of cellular biochemistry JO - J Cell Biochem VL - 112 IS - 5 N2 - Alveolar rhabdomyosarcoma (ARMS) are characterized by the expression of chimeric transcription factors Pax3-FKHR and Pax7-FKHR, due to chromosomal translocations fusing PAX3 or PAX7 with the FKHR gene. Although ARMS exhibits a muscle lineage phenotype, the cells evade terminal differentiation despite expressing the potent myogenic transcriptional regulator MyoD. Here we show that while Pax7-FKHR inhibits MyoD-dependent transcription, MyoD enhances Pax7-FKHR activity in myogenic cell cultures. Importantly, this effect is not recapitulated by close related transcription factor myogenin and involves specific MyoD functional domains, distinct from those required for Pax7 to regulate MyoD during muscle formation. Together, these results suggest that although repressed as a myogenic regulatory factor, MyoD can play an active role in ARMS by augmenting Pax7-FKHR function. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/21321994/Pax7_FKHR_transcriptional_activity_is_enhanced_by_transcriptionally_repressed_MyoD_ L2 - https://doi.org/10.1002/jcb.23057 DB - PRIME DP - Unbound Medicine ER -