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Characterization of a glutenin-specific serine proteinase of Sunn bug Eurygaster integricepts Put.
J Agric Food Chem. 2011 Mar 23; 59(6):2462-70.JA

Abstract

Glutenin hydrolyzing proteinases (GHPs) have been purified, by affinity chromatography, from wheat seeds damaged by the Sunn bug Eurygaster integriceps (Hemiptera, Scutelleridae). A 28 kDa protein was partially sequenced by mass spectrometry and Edman degradation which showed homology to serine proteases from various insects. Three full length clones were obtained from cDNA isolated from Sunn bug salivary glands using degenerate PCR based on the sequences obtained. The cleavage site of the protease was determined using recombinant and synthetic peptides and shown to be between the consensus hexapeptide and nonapeptide repeat motifs present in the high molecular weight subunits of wheat glutenin (PGQGQQ∧GYYPTSLQQ). Homology models were generated for the three proteinases identified in this study using the high resolution X-ray structure of a crayfish (Pontastacus leptodactylus) trypsin complexed with a peptide inhibitor as template (PDB accession 2F91). The novel specificity of this protease may find applications in both fundamental and applied studies.

Authors+Show Affiliations

All-Russian Institute for Plant Protection (VIZR), 3 Podbelsky, Pushkin, St. Petersburg 196608, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21323348

Citation

Konarev, Alexander V., et al. "Characterization of a Glutenin-specific Serine Proteinase of Sunn Bug Eurygaster Integricepts Put." Journal of Agricultural and Food Chemistry, vol. 59, no. 6, 2011, pp. 2462-70.
Konarev AV, Beaudoin F, Marsh J, et al. Characterization of a glutenin-specific serine proteinase of Sunn bug Eurygaster integricepts Put. J Agric Food Chem. 2011;59(6):2462-70.
Konarev, A. V., Beaudoin, F., Marsh, J., Vilkova, N. A., Nefedova, L. I., Sivri, D., Köksel, H., Shewry, P. R., & Lovegrove, A. (2011). Characterization of a glutenin-specific serine proteinase of Sunn bug Eurygaster integricepts Put. Journal of Agricultural and Food Chemistry, 59(6), 2462-70. https://doi.org/10.1021/jf103867g
Konarev AV, et al. Characterization of a Glutenin-specific Serine Proteinase of Sunn Bug Eurygaster Integricepts Put. J Agric Food Chem. 2011 Mar 23;59(6):2462-70. PubMed PMID: 21323348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a glutenin-specific serine proteinase of Sunn bug Eurygaster integricepts Put. AU - Konarev,Alexander V, AU - Beaudoin,Frédéric, AU - Marsh,Justin, AU - Vilkova,Nina A, AU - Nefedova,Ludmila I, AU - Sivri,Dilek, AU - Köksel,Hamit, AU - Shewry,Peter R, AU - Lovegrove,Alison, Y1 - 2011/02/16/ PY - 2011/2/18/entrez PY - 2011/2/18/pubmed PY - 2011/7/13/medline SP - 2462 EP - 70 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 59 IS - 6 N2 - Glutenin hydrolyzing proteinases (GHPs) have been purified, by affinity chromatography, from wheat seeds damaged by the Sunn bug Eurygaster integriceps (Hemiptera, Scutelleridae). A 28 kDa protein was partially sequenced by mass spectrometry and Edman degradation which showed homology to serine proteases from various insects. Three full length clones were obtained from cDNA isolated from Sunn bug salivary glands using degenerate PCR based on the sequences obtained. The cleavage site of the protease was determined using recombinant and synthetic peptides and shown to be between the consensus hexapeptide and nonapeptide repeat motifs present in the high molecular weight subunits of wheat glutenin (PGQGQQ∧GYYPTSLQQ). Homology models were generated for the three proteinases identified in this study using the high resolution X-ray structure of a crayfish (Pontastacus leptodactylus) trypsin complexed with a peptide inhibitor as template (PDB accession 2F91). The novel specificity of this protease may find applications in both fundamental and applied studies. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/21323348/Characterization_of_a_glutenin_specific_serine_proteinase_of_Sunn_bug_Eurygaster_integricepts_Put_ L2 - https://doi.org/10.1021/jf103867g DB - PRIME DP - Unbound Medicine ER -