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Antibody to granulocyte macrophage colony-stimulating factor reduces the number of activated tissue macrophages and improves left ventricular function after myocardial infarction in a rat coronary artery ligation model.
J Cardiovasc Pharmacol. 2011 May; 57(5):568-74.JC

Abstract

Granulocyte macrophage colony-stimulating factor (GM-CSF) promotes infarct expansion and inappropriate collagen synthesis in a myocardial infarction (MI). This study was designed to determine if treatment with anti-GM-CSF will inhibit macrophage migration, preserve function, and limit left ventricular (LV) remodeling in the rat coronary artery ligation model. Treatment with a monoclonal antibody to GM-CSF (5 mg/kg) was initiated 24 hours before coronary artery ligation and continued every 3 days for 3 weeks. Left coronary arteries of rats were ligated, animals were recovered, and cardiac function was evaluated 3 weeks postligation. Tissue samples were processed for histochemistry. Anti-GM-CSF treatment increased LV ejection fraction (37 ± 3% vs 47 ± 5%) and decreased LV end systolic diameter (0.75 ± 0.12 vs 0.59 ± 0.05 cm) with no changes in LV systolic pressure (109 ± 4 vs 104 ± 5 mm Hg), LV end diastolic pressure (22 ± 4 vs 21 ± 2 mm Hg), LV end diastolic diameter (0.96 ± 0.04 vs 0.92 ± 0.05 cm), or the time constant of LV relaxation tau (25.4 ± +2.4 vs 22.7 ± 1.4 milliseconds) (P < 0.05). Significantly lower numbers of tissue macrophages and significant reductions in infarct size were found in the myocardium of antibody-treated animals (81 ± 21.24 vs 195 ± 31.7 positive cells per 0.105 mm, compared with controls. These findings suggest that inhibition of macrophage migration may be beneficial in the treatment of heart failure after MI.

Authors+Show Affiliations

Development Engineering Sciences, LLC, Flagstaff, AZ, USA. rskellar@des-company.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21326109

Citation

Kellar, Robert S., et al. "Antibody to Granulocyte Macrophage Colony-stimulating Factor Reduces the Number of Activated Tissue Macrophages and Improves Left Ventricular Function After Myocardial Infarction in a Rat Coronary Artery Ligation Model." Journal of Cardiovascular Pharmacology, vol. 57, no. 5, 2011, pp. 568-74.
Kellar RS, Lancaster JJ, Thai HM, et al. Antibody to granulocyte macrophage colony-stimulating factor reduces the number of activated tissue macrophages and improves left ventricular function after myocardial infarction in a rat coronary artery ligation model. J Cardiovasc Pharmacol. 2011;57(5):568-74.
Kellar, R. S., Lancaster, J. J., Thai, H. M., Juneman, E., Johnson, N. M., Byrne, H. G., Stansifer, M., Arsanjani, R., Baer, M., Bebbington, C., Flashner, M., Yarranton, G., & Goldman, S. (2011). Antibody to granulocyte macrophage colony-stimulating factor reduces the number of activated tissue macrophages and improves left ventricular function after myocardial infarction in a rat coronary artery ligation model. Journal of Cardiovascular Pharmacology, 57(5), 568-74. https://doi.org/10.1097/FJC.0b013e318213258b
Kellar RS, et al. Antibody to Granulocyte Macrophage Colony-stimulating Factor Reduces the Number of Activated Tissue Macrophages and Improves Left Ventricular Function After Myocardial Infarction in a Rat Coronary Artery Ligation Model. J Cardiovasc Pharmacol. 2011;57(5):568-74. PubMed PMID: 21326109.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibody to granulocyte macrophage colony-stimulating factor reduces the number of activated tissue macrophages and improves left ventricular function after myocardial infarction in a rat coronary artery ligation model. AU - Kellar,Robert S, AU - Lancaster,Jordan J, AU - Thai,Hoang M, AU - Juneman,Elizabeth, AU - Johnson,Nicholle M, AU - Byrne,Howard G, AU - Stansifer,Maribeth, AU - Arsanjani,Reza, AU - Baer,Mark, AU - Bebbington,Christopher, AU - Flashner,Michael, AU - Yarranton,Geoffrey, AU - Goldman,Steven, PY - 2011/2/18/entrez PY - 2011/2/18/pubmed PY - 2011/9/17/medline SP - 568 EP - 74 JF - Journal of cardiovascular pharmacology JO - J Cardiovasc Pharmacol VL - 57 IS - 5 N2 - Granulocyte macrophage colony-stimulating factor (GM-CSF) promotes infarct expansion and inappropriate collagen synthesis in a myocardial infarction (MI). This study was designed to determine if treatment with anti-GM-CSF will inhibit macrophage migration, preserve function, and limit left ventricular (LV) remodeling in the rat coronary artery ligation model. Treatment with a monoclonal antibody to GM-CSF (5 mg/kg) was initiated 24 hours before coronary artery ligation and continued every 3 days for 3 weeks. Left coronary arteries of rats were ligated, animals were recovered, and cardiac function was evaluated 3 weeks postligation. Tissue samples were processed for histochemistry. Anti-GM-CSF treatment increased LV ejection fraction (37 ± 3% vs 47 ± 5%) and decreased LV end systolic diameter (0.75 ± 0.12 vs 0.59 ± 0.05 cm) with no changes in LV systolic pressure (109 ± 4 vs 104 ± 5 mm Hg), LV end diastolic pressure (22 ± 4 vs 21 ± 2 mm Hg), LV end diastolic diameter (0.96 ± 0.04 vs 0.92 ± 0.05 cm), or the time constant of LV relaxation tau (25.4 ± +2.4 vs 22.7 ± 1.4 milliseconds) (P < 0.05). Significantly lower numbers of tissue macrophages and significant reductions in infarct size were found in the myocardium of antibody-treated animals (81 ± 21.24 vs 195 ± 31.7 positive cells per 0.105 mm, compared with controls. These findings suggest that inhibition of macrophage migration may be beneficial in the treatment of heart failure after MI. SN - 1533-4023 UR - https://www.unboundmedicine.com/medline/citation/21326109/Antibody_to_granulocyte_macrophage_colony_stimulating_factor_reduces_the_number_of_activated_tissue_macrophages_and_improves_left_ventricular_function_after_myocardial_infarction_in_a_rat_coronary_artery_ligation_model_ L2 - https://doi.org/10.1097/FJC.0b013e318213258b DB - PRIME DP - Unbound Medicine ER -