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Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus.
J Allergy Clin Immunol 2011; 127(5):1148-54.e9JA

Abstract

BACKGROUND

Rhinoviruses are the major cause of asthma exacerbations. Previous studies suggest that primary bronchial epithelial cells (PBECs) from asthmatic subjects are more susceptible to rhinovirus infection because of deficient IFN-β production. Although augmenting the innate immune response might provide a novel approach for treatment of virus-induced asthma exacerbations, the potential of IFN-β to modulate antiviral and proinflammatory responses in asthmatic epithelium is poorly characterized.

OBJECTIVES

We sought to compare responses of PBECs from nonasthmatic and asthmatic subjects to exogenous IFN-β and test the inflammatory effects of IFN-β in response to rhinovirus infection.

METHODS

PBECs were treated with IFN-β and infected with a low inoculum of human rhinovirus serotype 1B to simulate a natural viral infection. Expression of interferon-responsive genes and inflammatory responses were analyzed by using reverse transcription-quantitative real-time PCR, cytometric bead arrays, or both; viral titers were assessed by using the 50% tissue culture infection dose.

RESULTS

Expression of IFN-β-stimulated antiviral genes was comparable in PBECs from nonasthmatic or asthmatic donors. Exogenous IFN-β significantly protected PBECs from asthmatic donors against rhinovirus infection by suppressing viral replication. Interferon-inducible protein 10 (IP-10), RANTES, and IL-6 release in response to rhinovirus infection was triggered only in PBECs from asthmatic donors. Although exogenous IFN-β alone stimulated some release of IP-10 (but not IL-6 or RANTES), it significantly reduced rhinovirus-induced IP-10, RANTES, and IL-6 expression when tested in combination with rhinovirus.

CONCLUSIONS

PBECs from asthmatic donors have a normal antiviral response to exogenous IFN-β. The ability of IFN-β to suppress viral replication suggests that it might limit virus-induced exacerbations by shortening the duration of the inflammatory response.

Authors+Show Affiliations

Division of Infection, Inflammation and Immunity, University of Southampton School of Medicine and National Institute for Health Research Respiratory Biomedical Research Unit, Southampton General Hospital, Southampton, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21329968

Citation

Cakebread, Julie A., et al. "Exogenous IFN-β Has Antiviral and Anti-inflammatory Properties in Primary Bronchial Epithelial Cells From Asthmatic Subjects Exposed to Rhinovirus." The Journal of Allergy and Clinical Immunology, vol. 127, no. 5, 2011, pp. 1148-54.e9.
Cakebread JA, Xu Y, Grainge C, et al. Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus. J Allergy Clin Immunol. 2011;127(5):1148-54.e9.
Cakebread, J. A., Xu, Y., Grainge, C., Kehagia, V., Howarth, P. H., Holgate, S. T., & Davies, D. E. (2011). Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus. The Journal of Allergy and Clinical Immunology, 127(5), pp. 1148-54.e9. doi:10.1016/j.jaci.2011.01.023.
Cakebread JA, et al. Exogenous IFN-β Has Antiviral and Anti-inflammatory Properties in Primary Bronchial Epithelial Cells From Asthmatic Subjects Exposed to Rhinovirus. J Allergy Clin Immunol. 2011;127(5):1148-54.e9. PubMed PMID: 21329968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus. AU - Cakebread,Julie A, AU - Xu,Yunhe, AU - Grainge,Chris, AU - Kehagia,Valia, AU - Howarth,Peter H, AU - Holgate,Stephen T, AU - Davies,Donna E, Y1 - 2011/02/16/ PY - 2010/07/05/received PY - 2010/12/06/revised PY - 2011/01/11/accepted PY - 2011/2/19/entrez PY - 2011/2/19/pubmed PY - 2011/7/21/medline SP - 1148 EP - 54.e9 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 127 IS - 5 N2 - BACKGROUND: Rhinoviruses are the major cause of asthma exacerbations. Previous studies suggest that primary bronchial epithelial cells (PBECs) from asthmatic subjects are more susceptible to rhinovirus infection because of deficient IFN-β production. Although augmenting the innate immune response might provide a novel approach for treatment of virus-induced asthma exacerbations, the potential of IFN-β to modulate antiviral and proinflammatory responses in asthmatic epithelium is poorly characterized. OBJECTIVES: We sought to compare responses of PBECs from nonasthmatic and asthmatic subjects to exogenous IFN-β and test the inflammatory effects of IFN-β in response to rhinovirus infection. METHODS: PBECs were treated with IFN-β and infected with a low inoculum of human rhinovirus serotype 1B to simulate a natural viral infection. Expression of interferon-responsive genes and inflammatory responses were analyzed by using reverse transcription-quantitative real-time PCR, cytometric bead arrays, or both; viral titers were assessed by using the 50% tissue culture infection dose. RESULTS: Expression of IFN-β-stimulated antiviral genes was comparable in PBECs from nonasthmatic or asthmatic donors. Exogenous IFN-β significantly protected PBECs from asthmatic donors against rhinovirus infection by suppressing viral replication. Interferon-inducible protein 10 (IP-10), RANTES, and IL-6 release in response to rhinovirus infection was triggered only in PBECs from asthmatic donors. Although exogenous IFN-β alone stimulated some release of IP-10 (but not IL-6 or RANTES), it significantly reduced rhinovirus-induced IP-10, RANTES, and IL-6 expression when tested in combination with rhinovirus. CONCLUSIONS: PBECs from asthmatic donors have a normal antiviral response to exogenous IFN-β. The ability of IFN-β to suppress viral replication suggests that it might limit virus-induced exacerbations by shortening the duration of the inflammatory response. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/21329968/Exogenous_IFN_β_has_antiviral_and_anti_inflammatory_properties_in_primary_bronchial_epithelial_cells_from_asthmatic_subjects_exposed_to_rhinovirus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(11)00101-1 DB - PRIME DP - Unbound Medicine ER -