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Determination of amphetamine-type stimulants, cocaine and ketamine in human hair by liquid chromatography/linear ion trap-Orbitrap hybrid mass spectrometry.
Analyst. 2011 Sep 07; 136(17):3503-11.A

Abstract

An LTQ Orbitrap XL hybrid mass spectrometry method was developed for the determination of illicit drugs and their metabolites, including amphetamine (AP), methamphetamine (MA), dimethylamphetamine (DMA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), ketamine (KET), norketamine (NK), cocaine (COC) and benzoylecgonine (BE), in hair. Micropulverized extraction was employed for sample preparation using a small hair sample (2 cm piece or 0.2 mg). Recoveries of the analytes during sample preparation were estimated using fortified hair samples and ranged from 35.5% for COC to 71.7% for AP. High resolution full-scan mass spectra and unit resolution product-ion spectra were obtained with the Orbitrap analyzer and the linear ion-trap analyzer, respectively. High-resolution extracted ion chromatograms at a tolerance of 3 ppm were utilized for quantification. The analytes were identified using the product-ion spectra in combination with the accurate masses of the corresponding protonated molecules observed in the high-resolution mass spectra. Lower limits of quantification obtained from a 0.2 mg hair sample were 0.050 ng mg(-1) (MDMA, KET and BE), 0.10 ng mg(-1) (AP, MA, DMA, NK and COC) and 0.50 ng mg(-1) (MDA). Two reference materials were analyzed for verification, and segmental analysis of single strands of hair specimens from actual cases was performed.

Authors+Show Affiliations

National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. miyaguchi@nrips.go.jpNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

21331390

Citation

Miyaguchi, Hajime, and Hiroyuki Inoue. "Determination of Amphetamine-type Stimulants, Cocaine and Ketamine in Human Hair By Liquid Chromatography/linear Ion trap-Orbitrap Hybrid Mass Spectrometry." The Analyst, vol. 136, no. 17, 2011, pp. 3503-11.
Miyaguchi H, Inoue H. Determination of amphetamine-type stimulants, cocaine and ketamine in human hair by liquid chromatography/linear ion trap-Orbitrap hybrid mass spectrometry. Analyst. 2011;136(17):3503-11.
Miyaguchi, H., & Inoue, H. (2011). Determination of amphetamine-type stimulants, cocaine and ketamine in human hair by liquid chromatography/linear ion trap-Orbitrap hybrid mass spectrometry. The Analyst, 136(17), 3503-11. https://doi.org/10.1039/c0an00850h
Miyaguchi H, Inoue H. Determination of Amphetamine-type Stimulants, Cocaine and Ketamine in Human Hair By Liquid Chromatography/linear Ion trap-Orbitrap Hybrid Mass Spectrometry. Analyst. 2011 Sep 7;136(17):3503-11. PubMed PMID: 21331390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamine-type stimulants, cocaine and ketamine in human hair by liquid chromatography/linear ion trap-Orbitrap hybrid mass spectrometry. AU - Miyaguchi,Hajime, AU - Inoue,Hiroyuki, Y1 - 2011/02/17/ PY - 2011/2/19/entrez PY - 2011/2/19/pubmed PY - 2011/12/13/medline SP - 3503 EP - 11 JF - The Analyst JO - Analyst VL - 136 IS - 17 N2 - An LTQ Orbitrap XL hybrid mass spectrometry method was developed for the determination of illicit drugs and their metabolites, including amphetamine (AP), methamphetamine (MA), dimethylamphetamine (DMA), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), ketamine (KET), norketamine (NK), cocaine (COC) and benzoylecgonine (BE), in hair. Micropulverized extraction was employed for sample preparation using a small hair sample (2 cm piece or 0.2 mg). Recoveries of the analytes during sample preparation were estimated using fortified hair samples and ranged from 35.5% for COC to 71.7% for AP. High resolution full-scan mass spectra and unit resolution product-ion spectra were obtained with the Orbitrap analyzer and the linear ion-trap analyzer, respectively. High-resolution extracted ion chromatograms at a tolerance of 3 ppm were utilized for quantification. The analytes were identified using the product-ion spectra in combination with the accurate masses of the corresponding protonated molecules observed in the high-resolution mass spectra. Lower limits of quantification obtained from a 0.2 mg hair sample were 0.050 ng mg(-1) (MDMA, KET and BE), 0.10 ng mg(-1) (AP, MA, DMA, NK and COC) and 0.50 ng mg(-1) (MDA). Two reference materials were analyzed for verification, and segmental analysis of single strands of hair specimens from actual cases was performed. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/21331390/Determination_of_amphetamine_type_stimulants_cocaine_and_ketamine_in_human_hair_by_liquid_chromatography/linear_ion_trap_Orbitrap_hybrid_mass_spectrometry_ L2 - https://doi.org/10.1039/c0an00850h DB - PRIME DP - Unbound Medicine ER -