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Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum.
Carbohydr Res 2011; 346(5):595-601CR

Abstract

Validamycin A was used to inhibit in vivo trehalase activity in tobacco enabling the study of subsequent changes in new C partitioning into cellulosic biomass and lignin precursors. After 12-h exposure to treatment, plants were pulse labeled using radioactive (11)CO(2), and the partitioning of isotope was traced into [(11)C]cellulose and [(11)C]hemicellulose, as well as into [(11)C]phenylalanine, the precursor for lignin. Over this time course of treatment, new carbon partitioning into hemicellulose and cellulose was increased, while new carbon partitioning into phenylalanine was decreased. This trend was accompanied by a decrease in phenylalanine ammonia-lyase activity. After 4d of exposure to validamycin A, we also measured leaf protein content and key C and N metabolite pools. Extended treatment increased foliar cellulose and starch content, decreased sucrose, and total amino acid and nitrate content, and had no effect on total protein.

Authors+Show Affiliations

Fachbereich Chemie, Johannes Gutenberg Universität, 55099 Mainz, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21333278

Citation

Best, Marcel, et al. "Inhibition of Trehalose Breakdown Increases New Carbon Partitioning Into Cellulosic Biomass in Nicotiana Tabacum." Carbohydrate Research, vol. 346, no. 5, 2011, pp. 595-601.
Best M, Koenig K, McDonald K, et al. Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum. Carbohydr Res. 2011;346(5):595-601.
Best, M., Koenig, K., McDonald, K., Schueller, M., Rogers, A., & Ferrieri, R. A. (2011). Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum. Carbohydrate Research, 346(5), pp. 595-601. doi:10.1016/j.carres.2011.01.018.
Best M, et al. Inhibition of Trehalose Breakdown Increases New Carbon Partitioning Into Cellulosic Biomass in Nicotiana Tabacum. Carbohydr Res. 2011 Apr 1;346(5):595-601. PubMed PMID: 21333278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum. AU - Best,Marcel, AU - Koenig,Kaitlyn, AU - McDonald,Kelly, AU - Schueller,Michael, AU - Rogers,Alistair, AU - Ferrieri,Richard A, Y1 - 2011/01/25/ PY - 2010/10/28/received PY - 2011/01/11/revised PY - 2011/01/19/accepted PY - 2011/2/22/entrez PY - 2011/2/22/pubmed PY - 2011/6/17/medline SP - 595 EP - 601 JF - Carbohydrate research JO - Carbohydr. Res. VL - 346 IS - 5 N2 - Validamycin A was used to inhibit in vivo trehalase activity in tobacco enabling the study of subsequent changes in new C partitioning into cellulosic biomass and lignin precursors. After 12-h exposure to treatment, plants were pulse labeled using radioactive (11)CO(2), and the partitioning of isotope was traced into [(11)C]cellulose and [(11)C]hemicellulose, as well as into [(11)C]phenylalanine, the precursor for lignin. Over this time course of treatment, new carbon partitioning into hemicellulose and cellulose was increased, while new carbon partitioning into phenylalanine was decreased. This trend was accompanied by a decrease in phenylalanine ammonia-lyase activity. After 4d of exposure to validamycin A, we also measured leaf protein content and key C and N metabolite pools. Extended treatment increased foliar cellulose and starch content, decreased sucrose, and total amino acid and nitrate content, and had no effect on total protein. SN - 1873-426X UR - https://www.unboundmedicine.com/medline/citation/21333278/Inhibition_of_trehalose_breakdown_increases_new_carbon_partitioning_into_cellulosic_biomass_in_Nicotiana_tabacum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-6215(11)00042-5 DB - PRIME DP - Unbound Medicine ER -