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Quantification of cyclizine and norcyclizine in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Mar 15; 879(9-10):605-9.JC

Abstract

A rapid and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay was developed and validated for quantification of cyclizine and its main metabolite norcyclizine in human plasma. Samples were prepared by protein precipitation with acetonitrile and cinnarizine was used as internal standard (recovery >87%). The analytes were eluted from a C8 50 mm×2.0 mm analytical column using a linear gradient of methanol and 0.05% formic acid with a total analysis time of 4 min. Analytes were detected by MS/MS using electrospray ionisation in the positive mode with multiple reactions monitoring (MRM) of the precursor ion/product ion transitions 267.2/167.2 for cyclizine and 253.2/167.2 for norcyclizine. Matrix effects were negligible. Standard curves for cyclizine and norcyclizine were linear (r(2)≥0.996) over the range 2-200 ng/mL, with 2 ng/mL representing the lower limit of quantification. Relative standard deviations were <14% for intra- and inter-day precision and the accuracy was within ±8%. The assay was successfully applied to a clinical study.

Authors+Show Affiliations

Clinical Pharmacology Research Group, Department of Medicine, University of Otago, Christchurch, PO Box 4345, Christchurch 8140, New Zealand. berit.jensen@cdhb.govt.nzNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21333615

Citation

Jensen, Berit Packert, et al. "Quantification of Cyclizine and Norcyclizine in Human Plasma By Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS)." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 879, no. 9-10, 2011, pp. 605-9.
Jensen BP, Vella-Brincat JW, Begg EJ. Quantification of cyclizine and norcyclizine in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS). J Chromatogr B Analyt Technol Biomed Life Sci. 2011;879(9-10):605-9.
Jensen, B. P., Vella-Brincat, J. W., & Begg, E. J. (2011). Quantification of cyclizine and norcyclizine in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 879(9-10), 605-9. https://doi.org/10.1016/j.jchromb.2011.01.021
Jensen BP, Vella-Brincat JW, Begg EJ. Quantification of Cyclizine and Norcyclizine in Human Plasma By Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS). J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Mar 15;879(9-10):605-9. PubMed PMID: 21333615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of cyclizine and norcyclizine in human plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS). AU - Jensen,Berit Packert, AU - Vella-Brincat,Jane Winifred Ann, AU - Begg,Evan James, Y1 - 2011/02/02/ PY - 2010/10/31/received PY - 2011/01/12/revised PY - 2011/01/20/accepted PY - 2011/2/22/entrez PY - 2011/2/22/pubmed PY - 2011/7/1/medline SP - 605 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 879 IS - 9-10 N2 - A rapid and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay was developed and validated for quantification of cyclizine and its main metabolite norcyclizine in human plasma. Samples were prepared by protein precipitation with acetonitrile and cinnarizine was used as internal standard (recovery >87%). The analytes were eluted from a C8 50 mm×2.0 mm analytical column using a linear gradient of methanol and 0.05% formic acid with a total analysis time of 4 min. Analytes were detected by MS/MS using electrospray ionisation in the positive mode with multiple reactions monitoring (MRM) of the precursor ion/product ion transitions 267.2/167.2 for cyclizine and 253.2/167.2 for norcyclizine. Matrix effects were negligible. Standard curves for cyclizine and norcyclizine were linear (r(2)≥0.996) over the range 2-200 ng/mL, with 2 ng/mL representing the lower limit of quantification. Relative standard deviations were <14% for intra- and inter-day precision and the accuracy was within ±8%. The assay was successfully applied to a clinical study. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/21333615/Quantification_of_cyclizine_and_norcyclizine_in_human_plasma_by_liquid_chromatography_tandem_mass_spectrometry__LC_MS/MS__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(11)00055-9 DB - PRIME DP - Unbound Medicine ER -