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Investigating the effects of adult neural stem cell transplantation by lumbar puncture in transient cerebral ischemia.
Neurosci Lett 2011; 495(1):1-5NL

Abstract

Stem cells have the ability to self renew and are therefore a good source for cell therapy following ischemia. In this study, we transplanted adult rat neural stem cells (NSCs) by lumbar puncture (LP) to investigate whether these cells can migrate and differentiate into neurons or glial cells, thereby improving functional outcome in cerebral ischemia. Transient ischemia was induced in adult rats (n=16) for 1h. Three days after the induction of ischemia, NSCs obtained from the subventricular zone of adult rats were injected into ischemic animals (n=8) by LP at the level of L6-S1. Improved recovery of the coordination of movement on the 1st, 7th, 14th, 21st and 28th days after the injury was examined by the Rotarod test and compared with non-transplanted ischemic animals (n=8). The presence of NSCs in the brain tissue of the animals was examined by immunohistofluorscence and immunohistochemical techniques. The coordination of movement in ischemic animals that received neural stem cells was improved significantly (P<0.05) compared with untreated ischemic animals. Cells labeled with PKH26 were observed in the ischemic area of brain tissue sections. The alkaline phosphatase test and immunohistochemical techniques demonstrated a gathering of NSCs in the lateral ventricle. A number of cells which expressed neuronal and astrocytic cell markers had migrated from the lateral ventricle to the subjacent brain parenchyma. NSCs injected by LP were able to migrate to the ischemic tissue and differentiate into neural-like cells. These differentiated cells may have improved the coordination in movement in the ischemic animals injected with NSCs.

Authors+Show Affiliations

Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran. seyyedjaafari@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21333715

Citation

Seyed Jafari, S S., et al. "Investigating the Effects of Adult Neural Stem Cell Transplantation By Lumbar Puncture in Transient Cerebral Ischemia." Neuroscience Letters, vol. 495, no. 1, 2011, pp. 1-5.
Seyed Jafari SS, Ali Aghaei A, Asadi-Shekaari M, et al. Investigating the effects of adult neural stem cell transplantation by lumbar puncture in transient cerebral ischemia. Neurosci Lett. 2011;495(1):1-5.
Seyed Jafari, S. S., Ali Aghaei, A., Asadi-Shekaari, M., Nematollahi-Mahani, S. N., & Sheibani, V. (2011). Investigating the effects of adult neural stem cell transplantation by lumbar puncture in transient cerebral ischemia. Neuroscience Letters, 495(1), pp. 1-5. doi:10.1016/j.neulet.2011.02.025.
Seyed Jafari SS, et al. Investigating the Effects of Adult Neural Stem Cell Transplantation By Lumbar Puncture in Transient Cerebral Ischemia. Neurosci Lett. 2011 May 9;495(1):1-5. PubMed PMID: 21333715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigating the effects of adult neural stem cell transplantation by lumbar puncture in transient cerebral ischemia. AU - Seyed Jafari,S S, AU - Ali Aghaei,A, AU - Asadi-Shekaari,M, AU - Nematollahi-Mahani,S N, AU - Sheibani,V, Y1 - 2011/02/17/ PY - 2010/10/05/received PY - 2011/02/09/revised PY - 2011/02/10/accepted PY - 2011/2/22/entrez PY - 2011/2/22/pubmed PY - 2011/9/14/medline SP - 1 EP - 5 JF - Neuroscience letters JO - Neurosci. Lett. VL - 495 IS - 1 N2 - Stem cells have the ability to self renew and are therefore a good source for cell therapy following ischemia. In this study, we transplanted adult rat neural stem cells (NSCs) by lumbar puncture (LP) to investigate whether these cells can migrate and differentiate into neurons or glial cells, thereby improving functional outcome in cerebral ischemia. Transient ischemia was induced in adult rats (n=16) for 1h. Three days after the induction of ischemia, NSCs obtained from the subventricular zone of adult rats were injected into ischemic animals (n=8) by LP at the level of L6-S1. Improved recovery of the coordination of movement on the 1st, 7th, 14th, 21st and 28th days after the injury was examined by the Rotarod test and compared with non-transplanted ischemic animals (n=8). The presence of NSCs in the brain tissue of the animals was examined by immunohistofluorscence and immunohistochemical techniques. The coordination of movement in ischemic animals that received neural stem cells was improved significantly (P<0.05) compared with untreated ischemic animals. Cells labeled with PKH26 were observed in the ischemic area of brain tissue sections. The alkaline phosphatase test and immunohistochemical techniques demonstrated a gathering of NSCs in the lateral ventricle. A number of cells which expressed neuronal and astrocytic cell markers had migrated from the lateral ventricle to the subjacent brain parenchyma. NSCs injected by LP were able to migrate to the ischemic tissue and differentiate into neural-like cells. These differentiated cells may have improved the coordination in movement in the ischemic animals injected with NSCs. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/21333715/Investigating_the_effects_of_adult_neural_stem_cell_transplantation_by_lumbar_puncture_in_transient_cerebral_ischemia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(11)00182-0 DB - PRIME DP - Unbound Medicine ER -