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Low-magnitude whole-body vibration does not enhance the anabolic skeletal effects of intermittent PTH in adult mice.
J Orthop Res. 2011 Apr; 29(4):465-72.JO

Abstract

Whole-body vibration (WBV) is a low-magnitude mechanical stimulus that may be anabolic for bone, yet we recently found that WBV did not improve bone properties in adult mice. Because intermittent parathyroid hormone (PTH) enhances the anabolic effects of high-magnitude skeletal loading, we sought to determine the skeletal effects of WBV in combination with PTH. Seven-month-old male BALB/c mice were assigned to six groups (n = 13-14/group) based on magnitude of applied acceleration (0 or 0.3 G) and PTH dose (0, 10, or 40 µg/kg/day). Mice were exposed to WBV (0.3 G, 90 Hz, sine wave) or sham loading (0 G) for 15 min/day, 5 days/week for 8 weeks. Vehicle or hPTH (1-34) was administered prior to each WBV session. Whole-body bone mineral content increased by ~ 5% from 0 to 8 weeks in the 40 µg/kg PTH group only, independent of WBV loading. Similarly, PTH treatment increased tibial cortical bone volume by ~5% from 0 to 8 weeks, independent of WBV loading. Neither PTH nor WBV stimulated trabecular bone formation. Consistent with the cortical bone effect, tibias from the 40 µg/kg PTH group had significantly greater ultimate force and energy to failure than tibias in the 0 and 10 µg/kg PTH groups, independent of WBV treatment. In summary, 8 weeks of intermittent PTH treatment increased cortical bone volume and strength in adult male BALB/c mice. Daily exposure to low-magnitude WBV by itself did not improve skeletal properties and did not enhance the PTH effect. No WBV-PTH synergy was found in this preclinical study.

Authors+Show Affiliations

Department of Orthopaedic Surgery, School of Medicine, Washington University, Saint Louis, Missouri 63110, USA. silvam@wustl.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21337386

Citation

Lynch, Michelle A., et al. "Low-magnitude Whole-body Vibration Does Not Enhance the Anabolic Skeletal Effects of Intermittent PTH in Adult Mice." Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, vol. 29, no. 4, 2011, pp. 465-72.
Lynch MA, Brodt MD, Stephens AL, et al. Low-magnitude whole-body vibration does not enhance the anabolic skeletal effects of intermittent PTH in adult mice. J Orthop Res. 2011;29(4):465-72.
Lynch, M. A., Brodt, M. D., Stephens, A. L., Civitelli, R., & Silva, M. J. (2011). Low-magnitude whole-body vibration does not enhance the anabolic skeletal effects of intermittent PTH in adult mice. Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 29(4), 465-72. https://doi.org/10.1002/jor.21280
Lynch MA, et al. Low-magnitude Whole-body Vibration Does Not Enhance the Anabolic Skeletal Effects of Intermittent PTH in Adult Mice. J Orthop Res. 2011;29(4):465-72. PubMed PMID: 21337386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Low-magnitude whole-body vibration does not enhance the anabolic skeletal effects of intermittent PTH in adult mice. AU - Lynch,Michelle A, AU - Brodt,Michael D, AU - Stephens,Abby L, AU - Civitelli,Roberto, AU - Silva,Matthew J, Y1 - 2010/11/08/ PY - 2010/07/08/received PY - 2010/09/13/accepted PY - 2011/2/22/entrez PY - 2011/2/22/pubmed PY - 2011/4/13/medline SP - 465 EP - 72 JF - Journal of orthopaedic research : official publication of the Orthopaedic Research Society JO - J Orthop Res VL - 29 IS - 4 N2 - Whole-body vibration (WBV) is a low-magnitude mechanical stimulus that may be anabolic for bone, yet we recently found that WBV did not improve bone properties in adult mice. Because intermittent parathyroid hormone (PTH) enhances the anabolic effects of high-magnitude skeletal loading, we sought to determine the skeletal effects of WBV in combination with PTH. Seven-month-old male BALB/c mice were assigned to six groups (n = 13-14/group) based on magnitude of applied acceleration (0 or 0.3 G) and PTH dose (0, 10, or 40 µg/kg/day). Mice were exposed to WBV (0.3 G, 90 Hz, sine wave) or sham loading (0 G) for 15 min/day, 5 days/week for 8 weeks. Vehicle or hPTH (1-34) was administered prior to each WBV session. Whole-body bone mineral content increased by ~ 5% from 0 to 8 weeks in the 40 µg/kg PTH group only, independent of WBV loading. Similarly, PTH treatment increased tibial cortical bone volume by ~5% from 0 to 8 weeks, independent of WBV loading. Neither PTH nor WBV stimulated trabecular bone formation. Consistent with the cortical bone effect, tibias from the 40 µg/kg PTH group had significantly greater ultimate force and energy to failure than tibias in the 0 and 10 µg/kg PTH groups, independent of WBV treatment. In summary, 8 weeks of intermittent PTH treatment increased cortical bone volume and strength in adult male BALB/c mice. Daily exposure to low-magnitude WBV by itself did not improve skeletal properties and did not enhance the PTH effect. No WBV-PTH synergy was found in this preclinical study. SN - 1554-527X UR - https://www.unboundmedicine.com/medline/citation/21337386/Low_magnitude_whole_body_vibration_does_not_enhance_the_anabolic_skeletal_effects_of_intermittent_PTH_in_adult_mice_ L2 - https://doi.org/10.1002/jor.21280 DB - PRIME DP - Unbound Medicine ER -