Tags

Type your tag names separated by a space and hit enter

Validated high-performance thin-layer chromatographic method for the quantification of thymoquinone in Nigella Sativa extracts and formulations.
Phytochem Anal. 2011 Jul-Aug; 22(4):367-73.PA

Abstract

INTRODUCTION

The surge of interest in naturally occurring phytochemicals with anticancer potential has led to the discovery of many molecules, one of them being thymoquinone (TQ) the bioactive constituent of the volatile oil of black seed, Nigella sativa L. (NS).

OBJECTIVE

The aim of the present work was to develop and validate an HPTLC method for determination of TQ in NS extracts, commercially available marketed oils, polyherbal formulations and in lipid-based oral and parenteral formulations prepared in-house.

METHODOLOGY

Analysis of TQ was performed on TLC aluminium plates pre-coated with silica gel 60F-254. Linear ascending development was carried out in twin trough glass chamber, saturated with mobile phase consisting of toluene-cyclohexane (8 : 2, v/v) at ambient temperature. Camag TLC scanner III was used for the spectrodensitometric scanning and analysis in absorbance mode at 254 nm.

RESULTS

The method was found to give compact spots for TQ (R(f) value of 0.28 ± 0.05) and was linear over the range 100-1400 ng/spot (r(2) = 0.9921 ± 0.0020). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at three concentrations were higher than 95% with RSD ≤ 3%.

CONCLUSION

The HPTLC method developed was found to be relatively simple, rapid and accurate for the routine analysis of TQ in extracts, marketed oils, polyherbal and in-house formulations.

Authors+Show Affiliations

Department of Pharmacology, Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai-400 098, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

21337651

Citation

Velho-Pereira, Reelma M., et al. "Validated High-performance Thin-layer Chromatographic Method for the Quantification of Thymoquinone in Nigella Sativa Extracts and Formulations." Phytochemical Analysis : PCA, vol. 22, no. 4, 2011, pp. 367-73.
Velho-Pereira RM, Barhate CR, Kulkarni SR, et al. Validated high-performance thin-layer chromatographic method for the quantification of thymoquinone in Nigella Sativa extracts and formulations. Phytochem Anal. 2011;22(4):367-73.
Velho-Pereira, R. M., Barhate, C. R., Kulkarni, S. R., & Jagtap, A. G. (2011). Validated high-performance thin-layer chromatographic method for the quantification of thymoquinone in Nigella Sativa extracts and formulations. Phytochemical Analysis : PCA, 22(4), 367-73. https://doi.org/10.1002/pca.1289
Velho-Pereira RM, et al. Validated High-performance Thin-layer Chromatographic Method for the Quantification of Thymoquinone in Nigella Sativa Extracts and Formulations. Phytochem Anal. 2011 Jul-Aug;22(4):367-73. PubMed PMID: 21337651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated high-performance thin-layer chromatographic method for the quantification of thymoquinone in Nigella Sativa extracts and formulations. AU - Velho-Pereira,Reelma M, AU - Barhate,Chandrashekhar R, AU - Kulkarni,Savita R, AU - Jagtap,Aarti G, Y1 - 2011/02/19/ PY - 2009/08/07/received PY - 2010/06/07/revised PY - 2010/09/23/accepted PY - 2011/2/22/entrez PY - 2011/2/22/pubmed PY - 2011/10/4/medline SP - 367 EP - 73 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 22 IS - 4 N2 - INTRODUCTION: The surge of interest in naturally occurring phytochemicals with anticancer potential has led to the discovery of many molecules, one of them being thymoquinone (TQ) the bioactive constituent of the volatile oil of black seed, Nigella sativa L. (NS). OBJECTIVE: The aim of the present work was to develop and validate an HPTLC method for determination of TQ in NS extracts, commercially available marketed oils, polyherbal formulations and in lipid-based oral and parenteral formulations prepared in-house. METHODOLOGY: Analysis of TQ was performed on TLC aluminium plates pre-coated with silica gel 60F-254. Linear ascending development was carried out in twin trough glass chamber, saturated with mobile phase consisting of toluene-cyclohexane (8 : 2, v/v) at ambient temperature. Camag TLC scanner III was used for the spectrodensitometric scanning and analysis in absorbance mode at 254 nm. RESULTS: The method was found to give compact spots for TQ (R(f) value of 0.28 ± 0.05) and was linear over the range 100-1400 ng/spot (r(2) = 0.9921 ± 0.0020). Accuracy, precision and repeatability were all within the required limits. The mean recoveries measured at three concentrations were higher than 95% with RSD ≤ 3%. CONCLUSION: The HPTLC method developed was found to be relatively simple, rapid and accurate for the routine analysis of TQ in extracts, marketed oils, polyherbal and in-house formulations. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/21337651/Validated_high_performance_thin_layer_chromatographic_method_for_the_quantification_of_thymoquinone_in_Nigella_Sativa_extracts_and_formulations_ L2 - https://doi.org/10.1002/pca.1289 DB - PRIME DP - Unbound Medicine ER -