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Tissue-type plasminogen activator depletion affects the nasal mucosa matrix reconstruction in allergic rhinitis mice.
Allergol Immunopathol (Madr). 2011 Jul-Aug; 39(4):206-11.AI

Abstract

BACKGROUND

The present study was designed to assess the function of tissue plasminogen activator (t-PA) expression in allergic rhinitis.

METHODS

Age-matched t-PA gene knock out (t-PA(-/-)) and wild type (WT) mice were sensitised four times, and then challenged for six weeks with ovalbumin. The controls were treated with saline instead of ovalbumin. The structural change in the nasal mucosa was investigated with haematoxylin and eosin stain and van Gieson staining. u-PA (urokinase-type plasminogen activator) and PAI-1 (plasminogen activator inhibitor) gene expression were measured by real time PCR. Matrix metalloproteinase-9 (MMP-9) expression was tested with Western blotting and with real time PCR.

RESULTS

After ovalbumin challenge for six weeks, compared with the WT group, t-PA depletion increased collagen deposition and gland hyperplasia. u-PA and PAI-1 gene expression increased both in t-PA(-/-) and in WT mice after ovalbumin treatment. MMP-9 expression decreased greatly after ovalbumin challenge in t-PA(-/-) mice.

CONCLUSION

t-PA affects the nasal mucosa matrix reconstruction process in allergic rhinitis, with which MMP-9 is involved.

Links

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elsevier.es
linkinghub.elsevier.com

Authors+Show Affiliations

Hua H
Department of Otorhinolaryngology, Huadong hospital, Shanghai medical college, Fudan university, Shanghai, China.
Zhang R
No affiliation info available
Yu S
No affiliation info available
Wang H
No affiliation info available
Zhao Y
No affiliation info available
Yu S
No affiliation info available
Li W
No affiliation info available

MeSH

Airway RemodelingAllergensAnimalsDisease Models, AnimalExtracellular MatrixGene Expression RegulationHumansHyperplasiaMatrix Metalloproteinase 9MiceMice, Inbred C57BLMice, KnockoutNasal MucosaOvalbuminPlasminogen Activator Inhibitor 1Rhinitis, Allergic, PerennialTissue Plasminogen ActivatorUrokinase-Type Plasminogen Activator

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21339035

Citation

Hua, H, et al. "Tissue-type Plasminogen Activator Depletion Affects the Nasal Mucosa Matrix Reconstruction in Allergic Rhinitis Mice." Allergologia Et Immunopathologia, vol. 39, no. 4, 2011, pp. 206-11.
Hua H, Zhang R, Yu S, et al. Tissue-type plasminogen activator depletion affects the nasal mucosa matrix reconstruction in allergic rhinitis mice. Allergol Immunopathol (Madr). 2011;39(4):206-11.
Hua, H., Zhang, R., Yu, S., Wang, H., Zhao, Y., Yu, S., & Li, W. (2011). Tissue-type plasminogen activator depletion affects the nasal mucosa matrix reconstruction in allergic rhinitis mice. Allergologia Et Immunopathologia, 39(4), 206-11. https://doi.org/10.1016/j.aller.2010.09.010
Hua H, et al. Tissue-type Plasminogen Activator Depletion Affects the Nasal Mucosa Matrix Reconstruction in Allergic Rhinitis Mice. Allergol Immunopathol (Madr). 2011 Jul-Aug;39(4):206-11. PubMed PMID: 21339035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue-type plasminogen activator depletion affects the nasal mucosa matrix reconstruction in allergic rhinitis mice. AU - Hua,H, AU - Zhang,R, AU - Yu,S, AU - Wang,H, AU - Zhao,Y, AU - Yu,S, AU - Li,W, Y1 - 2011/02/19/ PY - 2010/06/22/received PY - 2010/09/20/revised PY - 2010/09/28/accepted PY - 2011/2/23/entrez PY - 2011/2/23/pubmed PY - 2011/12/13/medline SP - 206 EP - 11 JF - Allergologia et immunopathologia JO - Allergol Immunopathol (Madr) VL - 39 IS - 4 N2 - BACKGROUND: The present study was designed to assess the function of tissue plasminogen activator (t-PA) expression in allergic rhinitis. METHODS: Age-matched t-PA gene knock out (t-PA(-/-)) and wild type (WT) mice were sensitised four times, and then challenged for six weeks with ovalbumin. The controls were treated with saline instead of ovalbumin. The structural change in the nasal mucosa was investigated with haematoxylin and eosin stain and van Gieson staining. u-PA (urokinase-type plasminogen activator) and PAI-1 (plasminogen activator inhibitor) gene expression were measured by real time PCR. Matrix metalloproteinase-9 (MMP-9) expression was tested with Western blotting and with real time PCR. RESULTS: After ovalbumin challenge for six weeks, compared with the WT group, t-PA depletion increased collagen deposition and gland hyperplasia. u-PA and PAI-1 gene expression increased both in t-PA(-/-) and in WT mice after ovalbumin treatment. MMP-9 expression decreased greatly after ovalbumin challenge in t-PA(-/-) mice. CONCLUSION: t-PA affects the nasal mucosa matrix reconstruction process in allergic rhinitis, with which MMP-9 is involved. SN - 1578-1267 UR - https://www.unboundmedicine.com/medline/citation/21339035/Tissue_type_plasminogen_activator_depletion_affects_the_nasal_mucosa_matrix_reconstruction_in_allergic_rhinitis_mice_ L2 - http://www.elsevier.es/en/linksolver/ft/pii/S0301-0546(11)00002-4 DB - PRIME DP - Unbound Medicine ER -